Abstract
Multiple buds and shoots were produced in vitro from leaf segments of Rubus idaeus L. (red raspberry) cultivars ‘Latham’, ‘Heritage’, ‘Festival’, and ‘Nova’. A liquid medium-containing bioreactor system combined with a gelled medium with 2.3–9.0 μM thidiazuron (TDZ) was used for bud and shoot regeneration. Shoot elongation was best in a medium containing 4.4 μM of 6-benzyladenine (BA). BA induced elongated shoots rooted in the bioreactor vessel containing the same medium but without any plant growth regulators. The growth and development of these tissue culture (TC) plants obtained from ‘Latham’ and ‘Festival’ raspberry cultivars were compared with those obtained by conventional root cuttings (RC), under field conditions. In the fourth and fifth years of growth, the TC plants produced more and longer canes and more berries with higher yield than those of RC plants in cultivar ‘Festival’ but not in ‘Latham’. However, simple sequence repeat (SSR) marker assay produced a homogenous amplification profile in the TC and donor control plants confirming the clonal fidelity of bioreactor-derived micropropagated plants in all four cultivars. Adventitious shoot regeneration in a bioreactor system containing liquid medium apparently induces the genotype-dependent juvenile branching characteristics. These juvenile branching characteristics favored enhanced vegetative growth and berry production but maintained clonal fidelity in micropropagated raspberries.
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References
Anderson WC (1980) Tissue culture propagation of red and black raspberry, Rubus idaeus and R. occidentalis. Acta Horticult 112:13–20
Cloutier S, Landry BS (1994) Molecular markers applied to plant tissue culture. In vitro cell. Dev Biol Plant 30:32–39
Compton EC (1994) Statistical methods suitable for the analysis of plant tissue culture data. Plant Cell Tissue Organ Cult 37:217–242
Crandall PC (1980) Twenty years of red raspberry research in southwestern Washington State. Acta Horticult 112:53–58
Dale A, Hughes BR, Donnelly D (2008) The role of micropropagation in producing specific pathogen-tested plants. HortSci 43:74–77
De Ancos B, Gonzales EM, Cano MP (2000) Ellagic acid, vitamin C, and total phenolic contents and radical scavenging capacity affected by freezing and frozen storage in raspberry fruit. J Agric Food Chem 48:4565–4570
Debnath SC (2010) A scaled-up system for in vitro multiplication of thidiazuron-induced red raspberry shoots using a bioreactor. J Hortic Sci Biotechnol 85:94–100
Debnath SC (2011) Bioreactors and molecular analysis in berry crop micropropagation—a review. Can J Plant Sci 91:147–157
Debnath SC, McRae KB (2001) An efficient in vitro shoot propagation of cranberry (Vaccinium macrocarpon Ait.) by axillary bud proliferation. In Vitro Cell Dev Biol Plant 37:243–249
Deng R, Donnelly DJ, Buszard DJ (1993) Field performance of micropropagated and conventionally propagated red raspberry. Acta Horticult 336:185–191
Donnelly DJ, Daubeny HA (1986) Tissue culture of Rubus species. Acta Horticult 183:305–314
George EF (1996) Plant propagation by tissue culture, part 2: in practice. Exegetics Ltd., Edington
Gomez KA, Gomez AA (1984) Statistical procedures for agricultural research, 2nd edn. Wiley, NY
Grout JM, Read PE, Wildung DK (1986) Influence of tissue culture and leaf-bud propagation on the growth habit of ‘Northblue’ blueberry. J Am Soc Hortic Sci 111:372–375
Hall HK, Hummer KE, Jamieson AR, Jennings SN, Weber CA (2009) Raspberry breeding and genetics. In: Janick J (ed) Plant breeding reviews, vol 32. Wiley, NY, pp 39–353
Henry Y, Nato A, DeBuyser J (1998) Genetic fidelity of plants regenerated from somatic embryos in cereals. In: Jain SM, Brar DS, Ahloowalia BS (eds) Somaclonal variation and induced mutations in crop improvement. Kluwer Acad Publ, Dordrecht, pp 65–80
Hoepfner AS, Nestby R, Nybom H (1996) Genetic deviation initiated by adventitious shoot regeneration from tissue cultured red raspberry. J Hortic Sci 71:71–79
Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tissue Organ Cult 33:105–119
James DJ, Knight VH, Thurbon IJ (1980) Micropropagation of red raspberry and the influence of phloroglucinol. Sci Hortic 12:313–319
James CM, Wilson F, Hadonou AM, Tobutt KR (2003) Isolation and characterization of polymorphic microsatellites in diploid strawberry (Fragaria vesca L.) for mapping, diversity studies and clone identification. Mol Ecol Notes 3:171–173
Jamieson AR, Nickerson NL (2003) Field performance of the lowbush blueberry propagated by seed, stem cuttings and micropropagation. Acta Horticult 626:431–436
Kalia RK, Rai MK, Kalia S, Singh R, Dhawan AK (2011) Microsatelite markers: an overview of the recent progress in plants. Euphytica 177:309–334
Lewers KS, Styan SMN, Hokanson SC, Bassil NV (2005) Strawberry genbank-derived and genomic simple sequence repeat (SSR) markers and their utility with strawberry, blackberry, and red and black raspberry. J Am Soc Hortic Sci 130:102–115
Logue S (1996) Genetic stability of microspore-derived doubled haploids. In: Jain SM, Sopory SK, Veilleux RE (eds) Haploid production of higher plants, vol 2. Kluwer Acad Publ, Dordrecht, pp 1–51
McNicol RJ, Graham J (1990) In vitro regeneration of Rubus from leaf and stem segments. Plant Cell Tissue Organ Cult 21:45–50
Millan-Mendoza B, Graham J (1999) Organogenesis and micropropagation in red raspberry using forchlorfenuron (CPPU). J Hortic Sci Biotechol 74:219–223
Minocha SC (1987) Plant growth regulators and morphogenesis in cell and tissue culture of forest trees. In: Bonga JM, Durzan DJ (eds) Cell and tissue culture in forestry, vol I. Martinus Nijhoff Publ, Dordrecht, pp 50–66
Morrison SE, Smagula JM (1986) Morphology, growth, and rhizome development of lowbush blueberry tissue culture plants, seedlings, and rooted softwood cuttings. HortSci 21:734
O’Dell CR (1989) Berries: marketing raspberries. Am Fruit Grow 109:34–35
Paek KY, Chakrabarty D, Hahn EJ (2005) Application of bioreactor systems for large scale production of horticultural and medicinal plants. Plant Cell Tissue Organ Cult 81:287–300
Read PE, Hartley CA, Sandahl JM, Wildung DK (1988) Field performance of in vitro propagated blueberries. Comb Proc Int Plant Prop Soc 37:450–452
Salvi ND, Geoge L, Eapen S (2001) Plant regeneration from the leaf base callus of turmeric and random amplified polymorphic DNA analysis of regenerate plants. Plant Cell Tissue Organ Cult 66:113–119
Scott TK (1972) Auxins and roots. Annu Rev Plant Physiol 23:235–258
Seeram NP, Adams LS, Zhang Y, Sand D, Heber D (2006) Blackberry, black raspberry, blueberry, cranberry, red raspberry and strawberry extracts inhibit growth and stimulate apoptosis of human cancer cells in vitro. J Agric Food Chem 54:9329–9339
Serres R, Klueh J, Stang E (1993) Influence of source propagule on rhizome production from lingonberry cuttings. Acta Horticult 346:178–182
Snir I (1981) Micropropagation of red raspberry. Sci Hortic 14:139–143
Sobczykiewicz D (1992) Micropropagation of raspberry (Rubus idaeus L.). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 18: high-tech and micropropagation II. Springer, Berlin, pp 339–353
Stafne ET, Clark JR, Weber CA, Graham J, Lewers KS (2005) Simple sequence repeat (SSR) markers for genetic mapping of raspberry and blackberry. J Am Soc Hortic Sci 130:722–728
Swartz HJ, Galletta GJ, Zimmerman RH (1983) Field performance and phenotypic stability of tissue culture-propagated thornless blackberries. J Am Soc Hortic Sci 108:285–290
Teisson C, Alvard D (1995) A new concept of plant in vitro cultivation liquid medium: temporary immersion. In: Terzi M, Cella R, Falavigna A (eds) Current plant science and biotechnology in agriculture, vol 22, current issues in plant molecular and cellular biology. Kluwer Acad Publ, Dordrecht, pp 105–110
Wang H, Nair MG, Strasburg M, Chang YC, Booren AM, Gray JI, DeWitt DL (1999) Antioxidant and antiinflammatory activities of anthocyanins and their aglycon, cyanidin, from tart cherries. J Nat Prod 62:294–296
Wood GA, Hall HK (2001) Source of Raspberry bushy dwarf virus in Rubus in New Zealand, and the infectibility of some newer cultivars to this virus. N Z J Crop Hortic Sci 29:177–186
Wu JH, Miller SA, Hall HK, Mooney PA (2009) Factors affecting the efficiency of micropropagation from lateral buds and shoot tips of Rubus. Plant Cell Tissue Organ Cult 99:17–25
Zawadzka M, Orlikowska T (2006) Increase in the quality of raspberry cultures under the influence of FeEDDHA. Acta Horticult 725:161–164
Ziv M (2005) Simple bioreactors for mass propagation of plants. Plant Cell Tissue Organ Cult 81:277–285
Acknowledgments
Atlantic Cool Climate Crop Research Centre contribution no. 225. The authors thank Drs. Shahrokh Khanizadeh, Chaim Kempler, and Andrew Jamieson of Agriculture and Agri-Food Canada for their helpful suggestions during the study period. Thanks are also due to Sarah Devine, Glen Chubbs, and Darryl Martin for their excellent technical help.
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Debnath, S.C. Bioreactor-induced adventitious shoot regeneration affects genotype-dependent morphology but maintains clonal fidelity in red raspberry. In Vitro Cell.Dev.Biol.-Plant 50, 777–788 (2014). https://doi.org/10.1007/s11627-014-9632-2
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DOI: https://doi.org/10.1007/s11627-014-9632-2