Skip to main content
SpringerLink
Log in

In vitro micropropagation and alkaloids of Hippeastrum vittatum

  • Plant Tissue Culture
  • Published:
In Vitro Cellular & Developmental Biology - Plant Aims and scope Submit manuscript

Abstract

Different concentrations of methyl jasmonate, spermine, casein hydrolysate, or progesterone combined with 16 mg/l 2 iP + 4 mg/l naphthalene acetic acid (NAA) were investigated in order to obtain higher multiplication rate and growth of Hippeastrum vittatum bulbs in vitro on MS basal medium. The highest multiplication rate (8.2 bulbs/explant) was attained with 80 mg/l spermine, while the highest bulb fresh weight (1.23 g/bulblet) was obtained with 4 mg/l methyl jasmonate. Progesterone at 20 mg/l or casein hydrolysate at 2.0 g/l gave the highest leaf length (14.1 and 13.2 cm, respectively). So, it can be advised to use 80 mg/l spermine combined with 16 mg/l 2 iP + 4 mg/l NAA to obtain the highest number of bulbs per explant with moderate leaf length and bulb fresh weight. Chemical analysis showed alternations in the alkaloid type ratio and number of compounds in the bulbs treated with methyl jasmonate (4 mg/l).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1.
Figure 2.
Figure 3.

Similar content being viewed by others

References

  • Ali A.; El Sayed H.; Abdallah O.; Steglich W. Oxocrinine and other alkaloids from Crinum americanum. Phytochemistry 25: 2399–2401; 1986.

    Google Scholar 

  • Ali A. A.; Mesbah M. K.; Frahm A. W. Phytochemical investigation of Hippeastrum vittatum. Part III: structural elucidation of hippadine. Planta Med. 43: 407–409; 1981.

    Article  PubMed  CAS  Google Scholar 

  • Ali A. A.; Mesbah M. K.; Frahm A. W. Phytochemical investigation of Hippeastrum vittatum. Part IV: stereochemistry of pancracine, the first 5,11-methanomorphanthridine alkaloid from Hippeastrum—structure of hippagine. Planta Med. 50: 188–189; 1984.

    Article  PubMed  CAS  Google Scholar 

  • Bais P. B.; Ravishankar G. A. Role of polyamines in ontogeny of plants and their biotechnological applications. Plant Cell Tissue Organ Cult. 69: 1–34; 2002.

    Article  CAS  Google Scholar 

  • Bastida J.; Lavilla R.; Viladomat F. Chemical and biological aspects of Narcissus alkaloids. In: Cordell G. A. (ed) The Alkaloids, vol. 63. Elsevier Scientific Publishing, Amsterdam, NL, pp 87–179; 2006.

    Google Scholar 

  • Bazabakana R.; Wattiez R.; Baucher M.; Diallo B.; Jaziri M. Effect of jasmonic acid on developmental morphology during in vitro tuberization of Dioscorea alata. L. Plant Growth Regul. 40: 229–237; 2003.

    Article  CAS  Google Scholar 

  • Berkov S.; Nikolova M.; Bastida J.; Viladomat F.; Codina C. Rapid TLC/GC-MS identification of acetylcholinesterase inhibitors in alkaloid extracts. Phytochem. Anal. 19: 411–419; 2008.

    Article  PubMed  CAS  Google Scholar 

  • Boit H. Amaryllidaceous alkaloids.XI. Alkaloids of Chlidanthus fragrans, Valotta purpurea, Nerine undulata, and Hippeastrum vittatum. Chem. Ber. 89: 1129–1134; 1989.

    Article  Google Scholar 

  • Bose T. K.; Yadav L. P. Commercial flowers. Naya Prokash, India; 1989.

    Google Scholar 

  • Creelman R. A.; Mullet J. E. Biosynthesis and action of jasmonates in plants. Annu. Rev. Plant Physiol. Plant Mol Biol 48: 355–381; 1997.

    Article  PubMed  CAS  Google Scholar 

  • Döpke W.; Bienert M. Alkaloids from Amaryllidaceae: the structure of oduline. Pharmazie 21: 323–324; 1966.

    Google Scholar 

  • El-Moghazi A.; Ali A. Microchemical identification of Amaryllidaceae alkaloids. Part I. Crinidine, vittatine, crinamine, powelline, hippacine, lycorine and BII. Planta Med. 30: 367–374; 1976

    Article  Google Scholar 

  • El-Moghazi A.; Ali A.; Mesbah M. Phytochemical investigation of Hippeastrum vittatum growing in Egypt. II. Isolation and identification of new alkaloids. Planta Med. 28: 336–342; 1975.

    Article  Google Scholar 

  • El-Shamy H. A. (2002) In vitro cultures studies on Bougainvillea plant. Ph. D. Thesis, Faculty of Agriculture, Zagazig University, Zagazig, Egypt

  • El-Shamy H. A. Effect of growth regulators and photoperiod on micropropagation of Amaryllis (Hippeastrum vittatum). The sixth Arabian Horticulture Conference. Ismailia, Egypt, pp 183–197; 2005.

    Google Scholar 

  • Feijoo M. C.; Iglesias I.; Gomez C. Jasmonic acid influences on in vitro propagation of Hyacinthoides paivae. Acta Hortic 486: 329–331; 1999.

    CAS  Google Scholar 

  • Feray A. A.; Hourmant A.; Penot M. Effect of polyamines on morphogenesis of in vitro potato plants (Solanum tuberosum cv. Bintje). Comp Rend Acad Sci 316: 1446–1451; 1993.

    CAS  Google Scholar 

  • Fiola J. A.; Swartz H. J. Somatic embryogenesis, organogenesis and proliferation in vitro from Rubus embryos. Acta Hortic 183: 91–98; 1986.

    Google Scholar 

  • Fountain W. M.; Rourke E. N. Investigations of in vitro culture of Hippeastrum as a method of rapid propagation. HortScience 15: 274–275; 1980.

    Google Scholar 

  • Gebhardt K.; Friedrich M. Micropropagation of Calluna vulgaris cv. Beale. Plant Cell Tissue Organ Cult 29: 137–145; 1987.

    Article  Google Scholar 

  • Ghosal S.; Kumar Y.; Chakrabarti D.; Lal J.; Singh S. Parasitism of Imperata cylindrical on Pancratium biflorum and the concomitant chemical changes in the host species. Phytochemistry 25: 1097–1102; 1986.

    Article  CAS  Google Scholar 

  • Iino M.; Nomura T.; Tamaki Y.; Yamada Y.; Yoneyama K.; Takeuchi Y.; Mori M.; Asami T.; Nakano T.; Yokota T. Progesterone: its occurrence in plants and involvement in plant growth. Phytochemistry 68: 1664–1667; 2007.

    Article  PubMed  CAS  Google Scholar 

  • Kim E. K.; Hahn E. J.; Murthy H. N.; Paek K. Y. High frequency of shoot multiplication and bulblet formation of garlic in liquid cultures. Plant Cell Tissue Organ Cult 73: 231–236; 2003.

    Article  Google Scholar 

  • Kreh M.; Matusch R.; Witte L. Capillary gas chromatography-mass spectrometry of Amaryllidaceae alkaloids. Phytochemistry 38: 773–776; 1995.

    Article  CAS  Google Scholar 

  • Little T. M.; Hills F. J. Agriculture experimentation design and analysis. Wiley, New York; 1978.

    Google Scholar 

  • Martin-Closas L.; Sol S.; Pelacho A. M. Potential application of jasmonic acid for Solanum tuberosum micropropagation. Acta Hortic 52: 127–133; 2000.

    Google Scholar 

  • Murashige T.; Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473–497; 1962.

    Article  CAS  Google Scholar 

  • Pavlov A.; Berkov S.; Courot E.; Gocheva T.; Tuneva D.; Pandova B.; Georgiev M.; Georgiev V.; Yanev S.; Burrus M.; Ilieva M. Galanthamine production by Leucojum aestivum in vitro systems. Process Biochem. 42: 734–739; 2007.

    Article  CAS  Google Scholar 

  • Pruski K.; Astatkie T.; Nowak J. Jasmonate effects on in vitro tuberization and tuber bulking in two potato cultivars (Solanum tuberosum L.) under different media and photoperiod conditions. In Vitro Cell Dev Biol Plant 38: 203–209; 2002.

    Article  CAS  Google Scholar 

  • Sabapathy S.; Nair H. In vitro propagation of taro with spermine, arginine and ornithine Ι. Plantlet regeneration from primary shoot apices and axillary buds. Plant Cell Rep. 11: 290–294; 1992.

    Article  CAS  Google Scholar 

  • Saker M.; Rady M.; El-Bahr M. Towards commercial production of ornamental bulbs in vitro. Egypt J Hortic 25: 113–128; 1998.

    CAS  Google Scholar 

  • Santos I.; Salema R. Promotion by jasmonic acid of bulb formation in shoot cultures of Narcissus triandrus L. Plant Growth Regul. 30: 133–138; 2000.

    Article  CAS  Google Scholar 

  • Silva A.; Andrade J.; Bevilaqua L.; Souza M.; Izquierdo I.; Amelia T.; Zuanazzi J.; Silveira J. Anxiolytic-, antidepressant- and anticonvulsant-like effects of the alkaloid montanine isolated from Hippeastrum vittatum. Pharmacol Biochem Behav 8: 148–154; 2006.

    Google Scholar 

  • Silva A.; Andrade J.; Machado K.; Rocha A.; Apel M.; Sobral M.; Henriques A.; Zuanazzi J. Screening for cytotoxic activity of extracts and isolated alkaloids from bulbs of Hypeastrum vittatum. Phytomedicine 15: 882–885; 2008.

    Article  PubMed  CAS  Google Scholar 

  • Tanimoto S.; Matsubara Y. Stimulating effect of spermine on bulblet formation in bulb-scale segments of Lilium longiflorum. Plant Cell Rep. 15: 297–300; 1995.

    Article  CAS  Google Scholar 

  • Wang F. M.; Xue Y. L. The changes in polyamine content and polyamine oxidase activity during in vitro bulblet formation from bulb scales of Lilium davidii var. Unicolor. Acta Phytophysiol Sin 14: 350–355; 1988.

    CAS  Google Scholar 

  • Youssef D. T. A. Alkaloids of the flowers of Hippeastrum vittatum. J. Nat. Prod. 64: 839–841; 2001.

    Article  PubMed  CAS  Google Scholar 

  • Youssef D. T. A. Cytotoxic phenolics from the flowers of Hippeastrum vittatum. Bulletin of Pharmaceutical Sciences, Assiut University 28: 143–148; 2005.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, 44519, Zagazig, Egypt

    Rawia Zayed

  2. Department of Pharmacognosy, Faculty of Pharmacy, Sinai University, 44551, North Sinai, Egypt

    Rawia Zayed

  3. Department of Horticulture, Faculty of Agriculture, Zagazig University, Zagazig, 44519, Egypt

    H. El-Shamy

  4. Departament de Productes Naturals, Biologia Vegetal i Edafologia, Facultat de Farmàcia, Universitat de Barcelona, Av. Joan XXIII s/n, 08028, Barcelona, Catalonia, Spain

    Strahil Berkov, Jaume Bastida & Carles Codina

  5. AgroBioInstitute, 8 Dragan Tzankov Blvd., 1164, Sofia, Bulgaria

    Strahil Berkov

Authors
  1. Rawia Zayed
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. El-Shamy
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Strahil Berkov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jaume Bastida
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Carles Codina
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Strahil Berkov.

Additional information

Editor: S. Murch

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zayed, R., El-Shamy, H., Berkov, S. et al. In vitro micropropagation and alkaloids of Hippeastrum vittatum . In Vitro Cell.Dev.Biol.-Plant 47, 695–701 (2011). https://doi.org/10.1007/s11627-011-9368-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11627-011-9368-1

Keywords

Search

Navigation