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High efficiency in vitro plant regeneration from epicotyl explants of Chinese peanut cultivars

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Abstract

An efficient organogenesis and micropropagation system was developed for in vitro plant regeneration of multiple cultivars of peanut (Arachis hypogaea). The system was used to regenerate plants from nine cultivars: Luhua no. 9, Luhua no. 13, Luhua no. 14, Fenghua no. 1, Fenghua no. 3, Fenghua no. 5, Huayu no. 23, Qinglan no. 2, and Baisha 1016. Epicotyl and embryo axis explants were cultured on Murashige and Skoog (MS) basal medium supplemented with 33.29–44.39 μM 6-benzyladenine (BAP) and 2.15–4.30 μM α-naphthaleneacetic acid (NAA). The highest rate of shoot formation was observed in epicotyl explants taken from 4-d-old seedlings (5.1 ± 1.4 shoots per explant). Optimum shoot development was observed in explants cultured on MS medium containing 4.48 μM BAP and 2.89 or 5.78 μM gibberellin (GA3). Well-developed shoots (3–5 cm high) formed roots after 2 wk on MS medium containing 0–2.69 μM NAA. We observed that all multiple shoots formed at the site of epicotyl incision and at the upper end of each section, indicating physiological polarity of shoot formation. The maximum shoot induction rate for Luhua no. 9, Luhua no. 13, Luhua no. 14, Fenghua no. 1, Fenghua no. 3, Fenghua no. 5, Huayu no. 23, Qinglan no. 2, and Baisha 1016 was 60.0%, 83.3%, 80.7%, 91.5%, 86.0%, 59.7%, 75.0%, 67.3%, and 72.7%, respectively. This regeneration system will play a vital role in achieving the genetic improvement of Chinese peanut.

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Acknowledgments

The authors are grateful to Professor Yongshan Wan of Shandong Agricultural University for kindly providing peanut cultivars and Mr. Qingqi Fan of Crop Research Institute, Shandong Academy of Agricultural Sciences for statistical analysis of the data. Thanks are also given to both Funds for Young Scientists (2006BS06024) and New Crop Variety Development and Industrialization Program supported by Shandong provincial government.

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Authors and Affiliations

  1. High-Tech Research Center, Shandong Academy of Agricultural Sciences, Jinan, 250100, China

    Lei Shan, Guiying Tang, Pingli Xu, Zhanji Liu & Yuping Bi

  2. Key Laboratory for Genetic Improvement of Crop, Animal and Poultry of Shandong Province, Jinan, 250100, China

    Lei Shan, Guiying Tang, Pingli Xu, Zhanji Liu & Yuping Bi

  3. Key Laboratory of Crop Genetic Improvement and Biotechnology, Huanghuaihai, Ministry of Agriculture, Jinan, 250100, China

    Lei Shan, Guiying Tang, Pingli Xu, Zhanji Liu & Yuping Bi

Authors
  1. Lei Shan
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  2. Guiying Tang
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  3. Pingli Xu
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  4. Zhanji Liu
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  5. Yuping Bi
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Correspondence to Lei Shan.

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Editor: N. J. Taylor

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Shan, L., Tang, G., Xu, P. et al. High efficiency in vitro plant regeneration from epicotyl explants of Chinese peanut cultivars. In Vitro Cell.Dev.Biol.-Plant 45, 525–531 (2009). https://doi.org/10.1007/s11627-009-9255-1

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  • DOI: https://doi.org/10.1007/s11627-009-9255-1

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