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Highly efficient in vitro adventitious shoot regeneration of peppermint (Mentha x piperita L.) using internodal explants

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Abstract

An in vitro regeneration system with a 100% efficiency rate was developed in peppermint [Mentha x piperita] using 5- to 7-mm-long second internode stem segments of 3-wk-old stock plants. Shoots developed at sites of excision on stem fragments either directly from the cells or via primary calluses. The optimal medium for maximum shoot initiation and regeneration contained Murashige and Skoog (MS) salts, B5 vitamins, thidiazuron (TDZ, 11.35 μM), ZT (4.54 μM), 10% coconut water (CW), 20 g l−1 sucrose, 0.75% agar, adjusted to pH 5.8. A frequency of 100% shoot initiation was achieved, with an average of 39 shoots per explant. This regeneration system is highly reproducible. The regenerated plants developed normally and were phenotypically similar to Black Mitcham parents.

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Acknowledgement

This work was supported by funding from the Mint Industrial Research Council and Center of Agricultural Resources, Institute of Genetics and Developmental Biology, CAS, China.

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Authors and Affiliations

  1. The State Key Laboratory of Plant Cell and Chromosome Engineering, Center of Agricultural Resources, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 286 Huaizhong Road, Shijiazhuang, Hebei, 050021, China

    Xiaohuan Wang, Zhenhua Gao, Yunzhen Wang & Xia Li

  2. Department of Horticulture and Landscape Architecture, Purdue University, 1165 Horticulture Building, West Lafayette, IN, 47907, USA

    Ray A. Bressan & Stephen C. Weller

  3. Center of Agricultural Resources, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 286 Huaizhong Road, Shijiazhuang, Hebei, 050021, China

    Xia Li

Authors
  1. Xiaohuan Wang
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  2. Zhenhua Gao
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  3. Yunzhen Wang
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  4. Ray A. Bressan
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  5. Stephen C. Weller
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  6. Xia Li
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Correspondence to Xia Li.

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Editor: J. Canhoto

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Wang, X., Gao, Z., Wang, Y. et al. Highly efficient in vitro adventitious shoot regeneration of peppermint (Mentha x piperita L.) using internodal explants. In Vitro Cell.Dev.Biol.-Plant 45, 435–440 (2009). https://doi.org/10.1007/s11627-008-9170-x

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  • DOI: https://doi.org/10.1007/s11627-008-9170-x

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