Abstract
Whole plants were regenerated from excised leaves of Drimiopsis kirkii Baker (Lily of the Valley) through direct somatic embryogenesis. An initial exposure to a low level of 2,4-dichlorophenoxyacetic acid (2,4-D, 0.45 μM) in the medium was essential in inducing the direct formation of somatic embryos. A high concentration of 2,4-D (4.52 μM) in the proliferation medium reduced embryogenesis and enhanced callus formation. The presence of kinetin in the medium enhanced the somatic-embryogenesis-inducing effect of 2,4-D (0.45 μM). The maximum embryogenesis rate (4,026 somatic embryos per gram of leaf) was obtained in explants cultured for 30 d in medium supplemented with 2.33 μM kinetin and 0.45 μM 2,4-D (embryo induction medium). Kinetin (4.65 μM) also enhanced embryo germination (97.6%), but the presence of α-naphthalene acetic acid in the medium drastically reduced embryo germination. Following conversion, the regenerated plantlets were transferred to soil and showed normal morphological characteristics.
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Editor: Gregory C. Phillips
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Lan, T.H., Hong, P.I., Huang, C.C. et al. High-frequency direct somatic embryogenesis from leaf tissues of Drimiopsis kirkii Baker (giant squill). In Vitro Cell.Dev.Biol.-Plant 45, 44–47 (2009). https://doi.org/10.1007/s11627-008-9161-y
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DOI: https://doi.org/10.1007/s11627-008-9161-y