Summary
A novel human cell line, TOM-2, was established from a rare uterine cervical cancer, glassy cell carcinoma (GCC). TOM-2 is the second established GCC cell line so far reported. The cells were intermediately or poorly differentiated with dysplastic nuclei and polygonal shape and secreted two tumor markers and cytokines, i.e., CA-125 and SCC, interleukin (IL)-1α, -6, and -8, and TNF-α. Growth of TOM-2 was so strongly dependent on population density that it was not possible to determine the plating efficiency. In mass culture, the following characteristics were observed: doubling time, 83 h; mode of chromosome number, 79; human papillomavirus type 18 DNA, detectable; tumorigenicity, easily transplantable into subcutis of nude mice; chemosensitivity in vitro, considerably sensitive to Cisplatin and 5-FU but not to 9 other antineoplastic agents. This novel cell line will be useful for developing new therapeutic strategies for the rare cancer, GCC.
Similar content being viewed by others
References
Albores-Saavedra, J.; Larraza, P.; Poucell, S., et al. Carcinoids of the uterine cervix: additional observation in a new tumor entity. Cancer 38:2328–2342; 1976.
Fujinaga, Y.; Shimada, M.; Okazawa, K., et al. Simultaneous detection and typing of genital human papillomavirus DNA using polymerase chain reaction. J. Gen. Virol. 72:1039–1044; 1991.
Fukumoto, S.; Matsumoto, T.; Harada, S., et al. Pheochromocytoma with pyrexia and marked inflammatory signs: a paraneoplastic syndrome with possible relation to interleukin-6 production. J. Clin. Endocrinol Metab. 73:877–881; 1991.
Glucksmann, A.; Cherry, C. P. Incidence, histology and response to radiation of mixed carcinoma (adenoacanthomas) of the uterine cervix. Cancer 9:971–979; 1956.
Harasawa, R.; Mizusawa, H.; Nozawa, K., et al. Detection and identification of dominant mycoplasma species in cell cultures by restriction analysis of the 16S-23S rRNA intergenic spacer regions. Res. Microbiol. 144:489–493; 1993.
Ishiwata, I.; Ishiwata, C.; Soma, M., et al. Differences between cell lines of uterine cervical glassy cell carcinoma and large cell non-keratinizing squamous cell carcinoma. Anal. Quant. Cytol. Histol. 12:290–298; 1990.
Ishiyama, M.; Shiga, M.; Sasamoto, K., et al. A new sulfonated tetrazolium salt that produces a highly water-soluble formazan dye. Chem. Pharm. Bull. (Tokyo) 41:1118–1122; 1993.
Kawano, M.; Hirano, T.; Matsuda, T., et al. Autocrine generation and requirement of BSF-2/IL-6 for human multiple myelomas. Nature 332(6159):83–85; 1988.
Littman, P.; Clement, P. B.; Henriksen, B., et al. Glassy cell carcinoma of the cervix. Cancer 37:2238–2246; 1976.
Matsuguchi, T.; Okamura, S.; Kawasaki, C., et al. Constitutive production of granulocyte colony-stimulating factor and interleukin-6 by a human lung cancer cell line, KSNY: gene amplification and increased mRNA stability. Eur. J. Haematol. 47:128–133; 1991.
Melhem, M. F.; Meisler, A. I.; Saito, R., et al. Cytokines in inflammatory malignant fibrous histiocytoma presenting with leukemoid reaction. Blood 82:2038–2044; 1993.
Mills, S. E.; Austin, M. B.; Randall, M. E. Lymphoepithelioma-like carcinoma of the uterine cervix: a distinctive, undifferentiated carcinoma with inflammatory stroma. Am. J. Surg. Pathol. 9:883–889; 1985.
Mizuno, K.; Sone, S.; Orino, E., et al. Spontaneous production of interleukin-8 by human lung cancer cells and its augmentation by tumor necrosis factor alpha and interleukin-1 at protein and mRNA levels. Oncology 51:467–471; 1994.
Muraki, J.; Nakazono, M. Establishment of a new human renal cell line (TC-1) and its productivity of interleukin-6 (IL-6) Nippon hinyokika gakkaizasshi 83:1882–1889; 1992.
Murashita, A.; Takasaki, Y.; Hashimoto, H., et al. A case of Raynoud’s disease with uterine cancer producing interleukin-6. Clin. Rheumatol. 11:410–412; 1992.
Nishide, K.; Nishida, M.; Arai, Y., et al. In vitro chemosensitivity of endometrial cancer considering the pharmacokinetics of each antitumor agent. J. Jpn. Soc. Cancer Ther. 31:365–375; 1996.
Novak, E. R. Cervical neoplasia. In: Novak, E. R.; Woodruff, J. D., ed. Novak’s gynecologic and obstetric pathology with clinical and endocrine relations. 8th Edition. Philadelphia: W. B. Saunders Co.; 1979:138–149.
Pak, H. Y.; Yokota, S. B.; Paladugu, R. R., et al. Glassy cell carcinoma of the cervix: cytologic and clinicopathological analysis. Cancer 52:307–312; 1983.
Pattillo, R. A.; Hussa, R. O.; Story, M. T., et al. Tumor antigen and human chorionic gonadotropin in CaSki cells: a new epidermoid cervical cancer cell line. Science 196:1456–1458; 1977.
Puck, T. T.; Marcus, P. I.; Cieciura, S. J. Clonal growth of mammalian cells in vitro: growth characteristics of colonies from single HeLa cells with and without a ‘feeder’ layer. J. Exp. Med. 103:273–284; 1956.
Tada, M.; Suzuki, K.; Yamakawa, Y., et al. Human glioblastoma cells produce 77 amino acid interleukin-8 (IL-8(77)). J. Neurooncol. 16:25–34; 1993.
Ulbright, T. M.; Gersell, D. J. Glassy cell carcinoma of the uterine cervix: a light and electron microscopic study of five cases. Cancer 51:2255–2263; 1983.
van Nagell, J. R.; Donaldson, E. S.; Wood, E. G., et al. Small cell cancer of the uterine cervix. Cancer 40:2243–2249; 1977.
Wiernik, P. H.; Schwartz, E. L.; Strauman, J. J., et al. Phase I clinical and pharmacokinetic study of taxol. Cancer Res. 47:2486–2493; 1987.
Woodworth, C. D.; Simpson, S. Comparative lymphokine secretion by cultured normal human cervical keratinocytes, papillomavirus-immortalized, and carcinoma cell lines. Am. J. Pathol. 142:1544–1555; 1993.
Wright, T. C.; Ferenczy, A.; Kurman, R. J. Carcinoma and other tumors of the cervix. In: Kurman, R. J., ed. Blaustein’s pathology of the female genital tract. 4th Edition. New York: Springer-Verlag; 19:279–326; 1994.
Yoshida, T.; Kawase, M.; Sasaki, K., et al. Comparative studies on the sensitivity of three methods for detecting mycoplasmal contamination in cell cultures. Bull. Jpn. Fed. Cult. Collect. 4:9–15; 1988.
Zaino, J. R.; Nahhas, A. W.; Mortel, R. Glassy cell carcinoma of the uterine cervix. Arch. Pathol. Lab. Med. 106:250–254; 1982.
zur Hausen, H. Papillomaviruses in anogenital cancer as a model to understand the role of viruses in human cancers. Cancer Res. 49:4677–4681; 1989.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Oki, A., Nishida, M., Satoh, T. et al. A novel human glassy-cell carcinoma cell line producing IL-6 and IL-8 from uterine cervix. In Vitro Cell.Dev.Biol.-Animal 34, 290–297 (1998). https://doi.org/10.1007/s11626-998-0005-1
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11626-998-0005-1