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References
Ahearn, M. J. Electron microscopy as an aid to the diagnosis and characterization of acute leukemia. In: Stass, S. A., ed. The acute leukemias: biologic, diagnostic, and therapeutic determinants. New York: Marcel Dekker, Inc.; 1987:87–130.
Ali, A. A.; Christensen, M. A.; Marcus, J. N., et al. The retinoblastoma protein in colorectal cancer. In: Rossini, F. P.; Winawer, S. J.; Lynch, H. T., eds. Recent progress in colorectal cancer: biology and management of high risk groups. Amsterdam, Netherlands: Elsevier Science Publishers B.V.; 1992:29–32.
Ali, A. A.; Marcus, J. N.; Harvey, J. P., et al. RB1 protein in normal and malignant human colorectal tissue and colon cancer cell lines. FASEB J. 7:931–937; 1993.
Baten, A.; Sakamoto, K.; Shamsuddin, A. M. Long-term culture of normal human colonic epithelial cells in vitro. FASEB J. 6:2726–2734; 1992.
Buset, M.; Winawer, S.; Friedman, E. Defining conditions to promote the attachment of adult human colonic epithelial cells. In Vitro Cell. Dev. Biol. 23:403–411; 1987.
Cheng, H.; Bjerknes, M.; Amar, J. Methods for the determination of epithelial cell kinetic parameters of human colonic epithelium isolated from surgical and biopsy specimens. Gastroenterology 86:78–85; 1984.
Furukawa, Y.; Decaprio, J. A.; Freedman, A., et al. Expression and state of phosphorylation of the retinoblastoma susceptibility gene product in cycling and noncycling human hematopoietic cells. Proc. Natl. Acad. Sci. USA 87:2770–2774; 1990.
Gibson, P. R.; Van De Pol, E.; Maxwell, L. E., et al. Isolation of colonic crypts that maintain structural and metabolic viability in vitro. Gastroenterology 96:283–291; 1989.
Ham, A. W. Histology. Philadelphia, PA: J. B. Lippincott Co.; 1969:679.
Hammond, S. L.; Ham, R. G.; Stampter, M. R. Serum-free growth of human mammary epithelial cells: rapid clonal growth in defined medium and extended serial passage with pituitary extract. Proc. Natl. Acad. Sci. USA 81:5435–5439; 1984.
Lechner, J. F.; LaVeck, M. A. A serum-free method for culturing normal human bronchial epithelial cells at clonal density. J. Tissue Culture Methods 9:43–48; 1985.
Lichtenberger, L. M.; Lechago, J.; Miller, T. A. Cell culture of human intestinal mucosae. Gastroenterology 77:1291–1300; 1979.
Lointier, P.; Wildrick, D. M.; Boman, B. M. Growth effects of tamoxifen on Lovo colon carcinoma cells and cultured cells from normal colonic mucosa. Anticancer Res. 12:1523–1526; 1992.
Moyer, M. P. Culture of human gastrointestinal epithelial cells. Proc. Soc. Exp. Biol. Med. 174:12–15; 1983.
Moyer, M. P.; Aust, J. B. Human colon cells: culture and in vitro transformation. Science 224:1445–1447; 1984.
Moyer, M. P.; Dixon, P. S.; Culpepper, A. L., et al. In vitro propagation and characterization of normal, preneoplastic, and neoplastic colonic epithelial cells. In: Moyer, M. P.; Poste, G. H., eds. Colon cancer cells. New York: Academic Press; 1990:85–136.
Stauffer, J. S.; Manzano, L. A.; Moyer, M. P. Colonic epithelial cell lines: a prelude to defining the molecular basis of progression in adenocarcinoma of the colon. Proc. Am. Assoc. Cancer Res. 34:36; 1993.
Stein, G. H.; Beeson, M.; Gordon, L. Failure to phosphorylate the retinoblastoma gene product in senescent human fibroblasts. Science 249:666–669; 1990.
Vogelstein, B.; Fearon, E. R.; Hamilton, S. R., et al. Genetic alterations during colorectal-tumor development. N. Engl. J. Med. 319:525–532; 1988.
Whitehead, R. H.; Brown, A.; Bhathal, P. S. A method for the isolation and culture of human colonic crypts in collagen gels. In Vitro Cell. Dev. Biol. 23:436–441; 1987.
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Wildrick, D.M., Roll, R., Lointier, P. et al. Isolation of normal human colonic mucosa: Comparison of methods. In Vitro Cell.Dev.Biol.-Animal 33, 18–27 (1997). https://doi.org/10.1007/s11626-997-0017-2
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DOI: https://doi.org/10.1007/s11626-997-0017-2