Summary
A technique for the isolation and culture of normal human bronchial epithelial (NHBE) cells is described. This procedure involves first explanting fragments of large airway tissue to initiate fibroblastic-cell-free outgrowths of epithelial cells and subsequently using a serum-free medium (LHC-9) to propagate the NHBE cells at clonal density.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
V. References
Autrup, H.; Harris, C. C. Metabolism of chemical carcinogens by human tissues. In: Harris, C. C.; Autrup, H., eds. Human Carcinogenesis. New York: Academic Press; 1983:169–194.
Averbach, O.; Stout, A. P.; Hammond. E. C. Changes in bronchial epithelium in relation to cigarette smoking and in relation to lung cancer. N Engl J. Med. 256:253–267; 1961.
Lechner, J. F.; Haugen, A.; Autrup, H. et al. Clonal growth of epithelial cells from normal adult human bronchus. Cancer Res. 41:2294–2304; 1981.
Lechner, J. F.; Haugen, A.; McClendon, I. A. et al. Clonal growth of normal adult human bronchial epithelial cells in a serum-free medium. In Vitro 18:633–642; 1982.
Lechner, J. F.; Haugen, A.; McClendon, I. A. Induction of squamous differentiation of normal bronchial epithelial cells by small amounts of serum. Differentiation 25:229–237; 1984.
Lechner, J. F.; Haugen, A.; Trump, B. F. et al. Effects of asbestos and carcinogenic metals on cultured human bronchial epithelium. In: Harris, C. C.; Autrup, H., eds. Human Carcinogenesis. New York; Academic Press; 1983:561–585.
Lechner, J. F.; McClendon, I. A.; LaVeck, M. A. et al. Differentiation control by platelet factors of squamous differentiation in normal and malignant human bronchial epithelial cells. Cancer Res. 43:5915–5921; 1983.
Lechner, J. F.; Tokiwa, T.; McClendon, I. A. et al. Effects of nickel sulfate on growth and differentiation of normal human bronchial epithelial cells. Carcinogenesis 5:1697–1703; 1984.
Leibovitz, A. Preparation of medium L-15. J. Tissue Cult. Methods 3:557–559; 1977.
Masui, T.; Wakefield, M. L.; LaVeck, M. A. Transforming growth factor-B (TGF-B) induces normal human bronchial epithelial cells to undergo squamous differentiation. In Vitro 21:58A;1985.
Peehl, D. M.; Ham, R. C. Clonal growth of human keratinocytes with small amounts of dialyzed serum. In Vitro 16:526–538; 1980.
Stoner, G. D.; Katoh, Y.; Foidart, J. et al. Identification and culture of human bronchial epithelial cells. Methods Cell Biol. 21A:15–35; 1980.
Willey, J. C.; LaVeck, M. A.; McClendon, I. A. et al. Relationship of ornithine decarboxylase activity and cAMP metabolism to proliferation of normal human bronchial epithelial cells. J. Cell Physiol. 124(2):207–212; 1985.
Willey, J. C.; Lechner, J. F.; Harris, C. C. Bombesin and the c-terminal tetradecapeptide of gastrin-releasing peptide are growth factors for normal human bronchial epithelial cells. Exp. Cell Res. 153:245–248; 1984.
Willey, J. C.; Moser, C. E.; Lechner, J. F. et al. Differential effects of 12-0-tetradecanoylphorbol-13-acetate on cultured normal and neoplastic human bronchial epithelial cells. Cancer Res. 44:5124–5126; 1984.
Willey, J. C.; Saladino, A. J.; Ozanne, C. et al. Acute effects of 12-0-tetradecanoylphorbol-13-acetate, teleocidin B, or 2,3,7,8-tetra-chlorodibenzo-p-droxin on cultured normal human bronchial epithelial cells. Carcinogenesis 5:209–215; 1984.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lechner, J.F., LaVeck, M.A. A serum-free method for culturing normal human bronchial epithelial cells at clonal density. Journal of Tissue Culture Methods 9, 43–48 (1985). https://doi.org/10.1007/BF01797773
Issue Date:
DOI: https://doi.org/10.1007/BF01797773