Abstract
This study examines the effects of ultraviolet-induced adipose tissue–derived mesenchymal stem cells and their supernatants on wound healing regarding cell viability, percentage of wound healing, released cytokine, and growth factors. It has been reported in previous studies that mesenchymal stem cells are resistant to ultraviolet light and have a protective effect on skin cells against ultraviolet-induced damage. At the same time, there are many studies in the literature about the positive effects of cytokines and growth factors secreted by mesenchymal stem cells. Based on this information, the effects of ultraviolet-induced adipose-derived stem cells and supernatants containing their secreted cytokines and growth factors on an in vitro two-dimensional wound model created with two different cell lines were investigated in this study. It was determined from the results that the highest cell viability and the least apoptotic staining were 100 mJ in mesenchymal stem cells (**p < 0.01). Furthermore, analysis of cytokines and growth factors collected from supernatants also supported 100 mJ as the optimal ultraviolet dose. It was observed that cells treated with ultraviolet and their supernatants significantly increased cell viability and wound-healing rate over time compared to other groups. In conclusion, with this study, it has been shown that adipose-derived stem cells exposed to ultraviolet light can have an important use in wound healing, both with their potential and with the more cytokines and growth factors they secrete. However, further analysis and animal experiments should be performed before clinical use.
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This research was supported by Hacettepe University Scientific Research Projects Coordination Unit (project no: FHD-2021–19583).
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EAÇ was responsible for the concept and design of the study. EHB and AYÖ performed the data acquisition and analysis. EAÇ was the responsible author who wrote the original draft. EHB, ŞE, AYÖ, and MAO made the final reading and corrections to the article.
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Part of this study was presented orally at the 26th International Biomedical Science and Technology Symposium (BIOMED 2022).
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Çetin, E.A., Babayiğit, E.H., Özdemir, A.Y. et al. Investigation of UV-treated mesenchymal stem cells in an in vitro wound model. In Vitro Cell.Dev.Biol.-Animal 59, 331–345 (2023). https://doi.org/10.1007/s11626-023-00772-4
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DOI: https://doi.org/10.1007/s11626-023-00772-4