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The effect of decellularized cartilage matrix scaffolds combined with endometrial stem cell–derived osteocytes on osteochondral tissue engineering in rats

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Abstract

Since decellularized tissues may offer the instructive niche for cell differentiation and function, their use as cell culture scaffolds is a promising approach for regenerative medicine. To repair osteochondral tissues, developing a scaffold with biomimetic structural, compositional, and functional characteristics is vital. As a result of their heterogeneous structure, decellularized articular cartilage matrix from allogeneic and xenogeneic sources are considered appropriate scaffolds for cartilage regeneration. We developed a scaffold for osteochondral tissue engineering by decellularizing sheep knee cartilage using a chemical technique. DNA content measurements and histological examinations revealed that this protocol completely removed cells from decellularized cartilage. Furthermore, SEM, MTS assay, and H&E staining revealed that human endometrial stem cells could readily adhere to the decellularized cartilage, and the scaffold was biocompatible for their proliferation. Besides, we discovered that decellularized scaffolds could promote EnSC osteogenic differentiation by increasing bone-specific gene expression. Further, it was found that decellularized scaffolds were inductive for chondrogenic differentiation of stem cells, evidenced by an up-regulation in the expression of the cartilage-specific gene. Also, in vivo study showed the high affinity of acellularized scaffolds for cell adhesion and proliferation led to an improved regeneration of articular lesions in rats after 4 weeks. Finally, a perfect scaffold with high fidelity is provided by the developed decellularized cartilage scaffold for the functional reconstruction of osteochondral tissues; these types of scaffolds are helpful in studying how the tissue microenvironment supports osteocytes and chondrocytes differentiation, growth, and function to have a good osteochondral repair effect.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

We would like to express our thanks to the Tehran University of Medical Sciences.

Funding

This work was supported by the Tehran University of Medical Sciences.

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Authors and Affiliations

  1. Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

    Naghmeh Bahrami

  2. Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran

    Naghmeh Bahrami

  3. Anatomical Sciences & Cognitive Neuroscience Department, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

    Sima bordbar

  4. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran

    Sima bordbar

  5. Immunogenetics Research Center, Department of Tissue Engineering & Regenerative Medicine, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran

    Elham Hasanzadeh

  6. Department of Tissue Engineering, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran

    Arash Goodarzi

  7. Dentistry Faculty, Tehran University of Medical Sciences, Tehran, Iran

    Armin Ai

  8. Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Abdolreza Mohamadnia

  9. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Abdolreza Mohamadnia

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  1. Naghmeh Bahrami
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  2. Sima bordbar
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  4. Arash Goodarzi
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  5. Armin Ai
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  6. Abdolreza Mohamadnia
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Correspondence to Armin Ai or Abdolreza Mohamadnia.

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Bahrami, N., bordbar, S., Hasanzadeh, E. et al. The effect of decellularized cartilage matrix scaffolds combined with endometrial stem cell–derived osteocytes on osteochondral tissue engineering in rats. In Vitro Cell.Dev.Biol.-Animal 58, 480–490 (2022). https://doi.org/10.1007/s11626-022-00692-9

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