Abstract
LncRNA non-coding repressor of NFAT (NRON) can promote bladder cancer and suppress liver cancer, while its role in esophageal squamous cell carcinoma (ESCC) is unknown. We analyzed its involvement in ESCC. NRON and miR-31 expression in plasma before and after cisplatin treatment was detected using RT-qPCR. The correlation between NRON and miR-31 was analyzed by linear regression. Expression of NRON and miR-31 in ESCC cells was also analyzed using RT-qPCR. Overexpression of NRON and miR-31 was achieved in ESCC cells to explore their interaction. Cell apoptosis induced by cisplatin was studied using cell apoptosis assay. NRON was highly expressed in ESCC and further upregulated after cisplatin treatment. MiR-31 was downregulated in ESCC and inversely correlated with NRON. In ESCC cells, NRON overexpression decreased miR-31 expression, while miR-31 failed to alter NRON expression. Cisplatin treatment promoted NRON expression and inhibited miR-31 expression. Under cisplatin treatment, cell apoptosis was inhibited after NRON overexpression and increased after miR-31 overexpression. Moreover, NRON inhibited the effect of miR-31 on cell apoptosis. NRON might promote the development of cisplatin resistance via downregulating miR-31 in ESCC.
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Acknowledgements
We thank Medical Innovation Fund of Fujian Health Administration and Education and Scientific Research Project of Young and middle-aged teachers in Fujian Education Department for approving.
Funding
This work was supported by the Medical Innovation Fund of Fujian Health Administration (Grant No. 2016-CX-31), the Natural Science Foundation of Fujian Province, China (Grant numbers 2020J01953), and the Young and Middle-aged Teachers Scientific Research Project of Education Department, Fujian Province, China (Grant numbers JAT190212).
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Bo Liu, Xu Li: study concepts, literature research, clinical studies, data analysis, experimental studies, manuscript writing and review; Jinbao Xie: study design, literature research, experimental studies and manuscript editing; Zhi Feng: definition of intellectual content, clinical studies, data acquisition and statistical analysis; Nanlong Lin: data acquisition, manuscript preparation and data analysis; Minjie Yu: data acquisition and statistical analysis.
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First Affiliated Hospital, Fujian Medical University Ethics Committee approved this study. All participants signed informed consent.
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Liu, B., Li, X., Xie, J. et al. LncRNA NRON negatively regulates cisplatin-induced cell apoptosis via downregulating miR-31 in esophageal squamous cell carcinomas. In Vitro Cell.Dev.Biol.-Animal 58, 37–43 (2022). https://doi.org/10.1007/s11626-021-00638-7
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DOI: https://doi.org/10.1007/s11626-021-00638-7