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Anti-aging effects of fetal dermal mesenchymal stem cells in a D-galactose-induced aging model of adult dermal fibroblasts

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Abstract

The main characteristic of skin aging is the change in the composition of the dermis, mainly resulting from fibroblast senescence. Mesenchymal stem cells derived from fetal dermis are defined as fetal dermal mesenchymal stem cells; they reportedly exert wound healing effects on the skin and regulate keloid fibroblast proliferation. D-Galactose is widely used in animal aging models. In this study, we confirmed that D-galactose inhibits adult dermal fibroblast proliferation, and the inhibitory effect gradually increased with increasing concentration. Finally, we chose a concentration of 40 g/L D-galactose to induce adult dermal fibroblast senescence. D-Galactose increased the intensity of senescence-associated β-galactosidase staining and the levels of reactive oxygen species in adult dermal fibroblasts. Furthermore, D-galactose increased the mRNA expression of p16, p21, and p53. The fetal dermal mesenchymal stem cell–conditioned medium improved the above-mentioned effects. Overall, fetal dermal mesenchymal stem cells exerted anti-aging effects against adult dermal fibroblasts induced by D-galactose via paracrine functions.

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Acknowledgements

We thank the members of the central laboratory of the Second Hospital of Shandong University.

Funding

This work was supported by the National Natural Science Foundation of China (81873934), Wang Zhengguo Foundation for Traumatic Medicine (growth factor rejuvenation project), Department of Education of Zhejiang Province, China (Y201942046), and Wenzhou Science and Technology Bureau (Y2021010).

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Authors and Affiliations

  1. Department of Emergency, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250012, Shandong, China

    Shengsheng Pan, Jingjuan Zhang, Shanshan Jia, Maoying Wang, Yi Pan & Duyin Jiang

  2. Department of Plastic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang, China

    Shengsheng Pan

  3. Department of Burns and Plastic Surgery, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, 250012, Shandong, China

    Shengsheng Pan, Xiao Wang & Duyin Jiang

  4. Department of Basic Medicine, Jining Medical University, Jining, 272067, Shandong, China

    Siyu Gong

Authors
  1. Shengsheng Pan
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  2. Siyu Gong
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  3. Jingjuan Zhang
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  4. Shanshan Jia
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  5. Maoying Wang
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  6. Yi Pan
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  7. Xiao Wang
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  8. Duyin Jiang
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Contributions

Shengsheng Pan designed the experiments, performed the experiments, analyzed the data, prepared the figures, and wrote the manuscript. Siyu Gong, Jingjuan Zhang, Shanshan Jia, and Maoying Wang performed the experiments, analyzed the data, and proofread the manuscript. Yi Pan and Xiao Wang performed the experiments. Duyin Jiang designed the experiments and supervised the research. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Duyin Jiang.

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Pan, S., Gong, S., Zhang, J. et al. Anti-aging effects of fetal dermal mesenchymal stem cells in a D-galactose-induced aging model of adult dermal fibroblasts. In Vitro Cell.Dev.Biol.-Animal 57, 795–807 (2021). https://doi.org/10.1007/s11626-021-00624-z

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  • DOI: https://doi.org/10.1007/s11626-021-00624-z

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