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Biogenic synthesis of Marsilea quadrifolia gold nanoparticles: a study of improved glucose utilization efficiency on 3T3-L1 adipocytes

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Abstract

This study aims mainly to provide an insight and understanding of the effect of glucose utilization efficiency of biogenic gold nanoparticles (GNPs) synthesized through the mediation of Marsilea quadrifolia (M. quadrifolia) methanol extract on 3T3-L1 adipocytes. The biosynthesized GNPs were characterized by UV visible spectrophotometry and FTIR. Simultaneously, the nature, stability, and morphological characteristics were analyzed by XRD, TG-DTA, SEM-EDS, HRTEM, and SAED. The results of characterization studies were used to assess the properties of GNPs. The in vitro cytotoxicity screening indicates that 100 μM of biogenic GNPs were displayed 71.23 ± 1.56% of cellular viability in 3T3-L1 adipocyte cells. Subsequently, increased glucose utilization of biosynthesized GNPs based on a dose-dependent manner on 3T3-L1 has also been demonstrated. The effect of GNPs (30 μg) on glucose uptake was higher than that of insulin and metformin. Moreover, the observed results clearly highlight that the biogenic GNPs have higher efficiency of glucose utilization and cellular viability in 3T3-L1 adipocytes with lower toxicity.

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Acknowledgements

The authors are grateful to Department of Chemical Engineering, Jadavpur University, Kolkata, India and Chancellor, Vice-chancellor, and Directors of Kalasalingam University, Krishnankoil, India for the research fellowships and utilizing research facilities. We thank Mr. P. Kathirvel, Mr. V. Krishna, and the Prabhu technicians for the FTIR, SEM, and XRD analysis and Sir CV Raman-KS Krishnan International Research Centre, Kalasalingam University, Krishnankoil, India and Sophisticated Test and Instrumentation Centre (STIC), Cochin University, Cochin, Kerala, India for the HRTEM analysis. We also thank Prof. Z. Maciej Gliwicz, Ms. Ewa Babkiwics, and Dr. Piotr Maszczyk, Department of Hydrobiology, Faculty of Biology, University of Warsaw, Warszawa, Poland, for their timely help and support.

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Authors and Affiliations

  1. Department of Chemical Engineering, Jadavpur University, Kolkata, 700032, India

    Anindita Chowdhury & Chiranjib Bhattacharjee

  2. Sir CV Raman-KS Krishnan International Research Centre, Kalasalingam University, Krishnankoil, 626126, India

    Selvaraj Kunjiappan, Balasubramanian Somasundaram & Theivendren Panneerselvam

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  1. Anindita Chowdhury
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  2. Selvaraj Kunjiappan
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  3. Chiranjib Bhattacharjee
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  4. Balasubramanian Somasundaram
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  5. Theivendren Panneerselvam
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Correspondence to Chiranjib Bhattacharjee.

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Editor: Tetsuji Okamoto

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Chowdhury, A., Kunjiappan, S., Bhattacharjee, C. et al. Biogenic synthesis of Marsilea quadrifolia gold nanoparticles: a study of improved glucose utilization efficiency on 3T3-L1 adipocytes. In Vitro Cell.Dev.Biol.-Animal 53, 483–493 (2017). https://doi.org/10.1007/s11626-017-0136-3

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