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Shinorine and porphyra-334 isolated from laver (Porphyra dentata) inhibit adipogenesis in 3T3-L1 cells

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Abstract

Mycosporine-like amino acids (MAAs) such as shinorine and porphyra-334 from Porphyra spp. are bioactive compounds with strong photoprotective and antioxidant properties. In this study, the anti-adipogenic effect of shinorine and porphyra-334 was examined in vitro utilizing 3T3-L1 preadipocytes. Shinorine and porphyra-334 were extracted from laver (Porphyra dentata) 50% methanolic (MeOH) extract of and their structures were elucidated by MS and NMR spectroscopy. Both compounds had no cytotoxic effect in 3T3-L1 cells (< 200 μg/mL) and inhibited the accumulation of lipid droplets in 3T3-L1 mature adipocytes in a dose-dependent manner (0.1 and 1.0 μM). Interestingly, both compounds had also significantly reduced the expression of adipogenic-related genes such as peroxisome proliferator-activated receptor γ2 (PPARγ2), CCAAT/enhancer-binding protein α (C/EBPα), adiponectin, and leptin in 3T3-L1 cells. The findings suggest that shinorine and porphyra-334 have the potential to inhibit adipogenesis in 3T3-L1 preadipocytes.

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Acknowledgements

This research was supported by the Ministry of Oceans and Fisheries and Mokpo Metropolitan City through the Capacity Building Project for the Development of Mokpo Seafood Industry-Based Complex (No. B0080416003290) and the National Research Foundation (NRF) funded by the Ministry of Science and ICT (MSIT), Republic of Korea (No. 2019R1I1A3A01059211).

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Author notes

  1. Su-Young Choi and Su Yeon Lee have equally contributed in this study.

Authors and Affiliations

  1. Department of Animal Science, Chonnam National University, 77 Yongbongro, Gwangju, 61186, Republic of Korea

    Su-Young Choi, Don Carlo Batara & Sung-Hak Kim

  2. Department of Food Science and Technology, Chonnam National University, 77 Yongbongro, Gwangju, 61186, Republic of Korea

    Su Yeon Lee & Jeong-Yong Cho

  3. Mokpo Marine Food-Industry Research Center, Mokpo, 58621, Republic of Korea

    Hyung Gyun Kim & Jae Cheon Jeong

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  1. Su-Young Choi
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  2. Su Yeon Lee
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Correspondence to Sung-Hak Kim or Jeong-Yong Cho.

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Choi, SY., Lee, S.Y., Kim, H.G. et al. Shinorine and porphyra-334 isolated from laver (Porphyra dentata) inhibit adipogenesis in 3T3-L1 cells. Food Sci Biotechnol 31, 617–625 (2022). https://doi.org/10.1007/s10068-022-01055-6

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  • DOI: https://doi.org/10.1007/s10068-022-01055-6

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