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Effect of C-type natriuretic peptide pretreatment on in vitro bovine oocyte maturation

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Abstract

C-type natriuretic peptide (CNP) has been considered as a physiological meiotic inhibitor that stimulates the cGMP production by cumulus cell natriuretic peptide receptor 2 (NPR2), which inhibits oocyte phosphodiesterase type 3 activity and increases cAMP. In this study, we explored the effect of CNP pretreatment on the in vitro maturation (IVM) of bovine oocytes by examining changes in cleavage rate, blastocyst formation, mitochondrial DNA (mtDNA) copy number, reactive oxygen species (ROS) level, glutathione (GSH) content, and redox state. Our results showed that 200 nM CNP could effectively maintain meiotic arrest of bovine oocytes in vitro within 6 h. The two-step IVM system in which oocytes were pretreated with 200 nM CNP for 6 h and then cultured IVM for 28 h yielded a significantly (P < 0.05) increased blastocyst rate and cell number after in vitro fertilization (IVF) while compared to the conventional one-step IVM method. In addition, in comparison with the conventional 24-h matured oocyte, oocytes pretreated with 200 nM CNP for 6 h followed by 28 h IVM resulted in significantly (P < 0.05) higher mtDNA copy number and ROS levels in oocytes, while GSH level significantly (P < 0.05) decreased. Remarkably, regardless of treatment, no changes were observed in FAD++, NAD(P)H autofluorescence intensity, and redox ratio (FAD++/NAD(P)H) within the oocytes, maintaining a healthy metabolic equilibrium of redox throughout the two-step IVM. In conclusion, these results indicate that CNP pretreatment could dramatically improve the quality of bovine oocytes during in vitro maturation.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 31460598) and the Natural Science Foundation of Inner Mongolia Autonomous Region of China (No. 2014MS0327).

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Authors and Affiliations

  1. Key Laboratory of Animal Genetics, Breeding and Reproduction, College of Animal Science, Inner Mongolia Agricultural University, 306 Zhaowuda Road, Hohhot, Inner Mongolia, 010018, China

    Tong Zhang, Chunqiang Zhang, Xiaomei Fan, Ruilan Li & Jiaxin Zhang

  2. Wulanchabu Medical College, Wulanchabu, Inner Mongolia, 012000, China

    Chunqiang Zhang

  3. Basic Medical College, Inner Mongolia Medical University, Hohhot, Inner Mongolia, 010110, China

    Xiaomei Fan

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  1. Tong Zhang
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  2. Chunqiang Zhang
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  3. Xiaomei Fan
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  4. Ruilan Li
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  5. Jiaxin Zhang
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Correspondence to Jiaxin Zhang.

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Editor: Tetsuji Okamoto

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Zhang, T., Zhang, C., Fan, X. et al. Effect of C-type natriuretic peptide pretreatment on in vitro bovine oocyte maturation. In Vitro Cell.Dev.Biol.-Animal 53, 199–206 (2017). https://doi.org/10.1007/s11626-016-0101-6

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  • DOI: https://doi.org/10.1007/s11626-016-0101-6

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