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Adipose-derived stem cells extract has a proliferative effect on myogenic progenitors

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Abstract

Finding an effective method to regenerate muscle is a growing issue in the orthopedic field. Platelet-rich plasma (PRP) has recently been considered for therapeutic use due to its capacity to induce proliferation of myogenic progenitor cells (MPCs). Adipose-derived stem cells (ASCs) and its extract are regarded as a promising treatment for various disorders within the orthopedic field but their therapeutic relevance in the muscle regeneration is poorly investigated. In this study, rabbit MPCs were cultured from the supraspinatus of rabbit and characterized by myogenic markers. To investigate the paracrine effect of ASCs on MPCs, coculture experiments were performed. In order to see the anabolic effect of ASC-extracts (ASC-ex) in MPCs, cell proliferation assays were performed and compared with the PRP-added condition. Coculture experiment showed ASCs had an anabolic paracrine effect on proliferation of MPCs. PRP had a positive effect on proliferation of MPCs when compared to the control (100 ± 7.4% vs 195.2 ± 19.2%, p < 0.001); however, ASC-ex promoted greater proliferation than the PRP condition (467.3 ± 38.7%, p < 0.001 compared with PRP). Similarly, in C2C12 cells, PRP showed an increased rate when compared to the control (100 ± 5.9% vs 205.1 ± 45.4%, p < 0.001), and treatment of ASC-ex showed dramatic increase in proliferation (335.9 ± 37.8%, p < 0.001 compared with PRP). ASC-ex had positive effect on expanding MPCs of rabbit and myoblast cell line, and its capacity to induce proliferation was notably stronger than that of PRP. In conclusion, the study suggests that rabbit ASC-ex have stronger proliferative effect on MPCs than rabbit PRP. Thus, ASC-ex could be a therapeutic candidate for muscle regeneration by activation of endogenous MPCs.

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Authors and Affiliations

  1. Department of Neurology, Biomedical Research Institute, Seoul National University Hospital, Seoul, South Korea

    Wooseok Im, Jae-Jun Ban, Jiyeon Lim & Mijung Lee

  2. Department of Veterinary Internal Medicine and Geriatrics, Kangwon National University, Chuncheon, South Korea

    Jin Young Chung

  3. Clinical Physiology Laboratory, Department of Orthopaedic Surgery, UCSD Medical Center, San Diego, CA, USA

    Roshmi Bhattacharya

  4. Department of Orthopaedic Surgery, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, South Korea

    Sae Hoon Kim

  5. Department of Orthopaedic Surgery, Seoul National University Hospital, 101 Daehakro, Chongnogu, Seoul, 110-744, South Korea

    Sae Hoon Kim

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  1. Wooseok Im
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  2. Jae-Jun Ban
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  3. Jiyeon Lim
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  4. Mijung Lee
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  5. Jin Young Chung
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Correspondence to Sae Hoon Kim.

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Editor: T. Okamoto

Wooseok Im and Jae-Jun Ban contributed equally.

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Im, W., Ban, JJ., Lim, J. et al. Adipose-derived stem cells extract has a proliferative effect on myogenic progenitors. In Vitro Cell.Dev.Biol.-Animal 50, 740–746 (2014). https://doi.org/10.1007/s11626-014-9752-3

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  • DOI: https://doi.org/10.1007/s11626-014-9752-3

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