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Protocol for Obtaining Platelet-Rich Plasma (PRP), Platelet-Poor Plasma (PPP), and Thrombin for Autologous Use

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Abstract

Background

Plasma has been widely studied and used in many different situations to speed up healing with better tissue adherence and hemostasis. Research projects are now attempting to isolate platelet-rich plasma (PRP) and platelet-poor plasma (PPP), making better use of their properties, particularly during operations and for wounds that are slow to heal. In view of the wide diversity of industrial machines and extraction protocols, together with the variety of industrially produced biologic glues, this article suggests an option for obtaining PRP, PPP, and human thrombin for autologous use.

Methods

A way of obtaining PRP, PPP, and thrombin is reproduced through a protocol defined and established by the authors. Autologous thrombin and plasma were obtained through the collection and successive centrifugation of ten whole blood samples, until the desired hemocomponents were isolated, followed by quantitative and qualitative analyses of the elements obtained.

Results

The mean platelet concentration obtained was 6.03 × 108 platelets/ml, with a mean thrombin concentration of 33.54 nM, both values compatible with reports in the literature when different protocols are applied.

Conclusions

The protocol described is a good option for the preparation and application of PRP, PPP, and autologous thrombin, particularly as they can be obtained simultaneously, eliminating the possibilities of viral contamination and allergic reactions. Moreover, the cost of this procedure is low, it is easy to perform, and replicable.

Level of Evidence IV

This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266.

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Conflict of interest

The authors have no conflicts of interest or financial ties to disclose.

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Authors and Affiliations

  1. Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

    Diogo Franco, Talita Franco, Angélica Maria Schettino & João Medeiros Tavares Filho

  2. Federal University of Pará, Pará, Brazil

    Fabiel Spani Vendramin

  3. Rua Ramon Franco, 98-Urca, Rio de Janeiro, 22.290-290, Brazil

    Diogo Franco

Authors
  1. Diogo Franco
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  2. Talita Franco
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  3. Angélica Maria Schettino
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  4. João Medeiros Tavares Filho
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  5. Fabiel Spani Vendramin
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Correspondence to Diogo Franco.

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Franco, D., Franco, T., Schettino, A.M. et al. Protocol for Obtaining Platelet-Rich Plasma (PRP), Platelet-Poor Plasma (PPP), and Thrombin for Autologous Use. Aesth Plast Surg 36, 1254–1259 (2012). https://doi.org/10.1007/s00266-012-9957-3

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  • DOI: https://doi.org/10.1007/s00266-012-9957-3

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