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Developmental potency of pre-implant parthenogenetic goat embryos: effect of activation protocols and culture media

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Abstract

The developmental potency of pre-implant parthenogentic goat embryos were compared under two chemical activation protocols in three different culture media groups. The in vitro matured oocytes were chemically activated by two protocols viz. P1 (CB-CHX-6DMAP) and P2 (Ca-CHX-6DMAP). The activated oocytes under both the protocols were developed in three culture media, viz. modified synthetic oviductal fluid (mSOF), research vitro cleave medium (RVCL), and RVCL-Blast. While comparing the developmental potential of activated oocytes, it was observed that the oocytes activated under P2 protocol pooled over three culture media group producing significantly higher mean cleavage rate (43.2 ± 0.9 vs 40.6 ± 1.5), blastocyst development (16.4 ± 1.1 vs 12.6 ± 0.8), and blastomere count (120.7 ± 4.7 vs 113.2 ± 4.1) as compared to P1 protocol. The comparison of effect of culture media pooled over protocol groups revealed that the mean cleavage rate observed under RVCL-Blast (44.8 ± 1.3) and RVCL (45.3 ± 0.5) were significantly higher (P ≤ 0.01) than mSOF (35.8 ± 1.2). However, the mean blastocyst development observed under RVCL-Blast group (18.8 ± 3.2) was significantly higher than RVCL (14.0 ± 0.8) and mSOF (10.8 ± 0.4). Similarly, the mean blastomere count under RVCL-Blast group (136.0 ± 3.7) was significantly higher (P ≤ 0.01) than RVCL (114.7 ± 1.0) and mSOF (100.2 ± 0.5) groups. The semiquantitative RT PCR analysis showed the expression of pro-apoptotic caspase 3 gene in P1 and anti-apoptotic Mcl-1 gene in P2. This study concludes that the activation protocol P2 and embryo cultured under RVCL-Blast group were optimum for chemical activation and culture medium, respectively.

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Acknowledgments

The authors are thankful for the financial support provided by National Agricultural Innovation Project, Indian Council of Agricultural Research, Government of India, New Delhi.

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  1. Nanaji Deshmukh Veterinary Sciences University, Jabalpur, Madhya Pradesh, India

    Dharmendra Kumar, R. Gopalakrishna, Ajay P. Singh, Rakesh Ranjan, Saurabh K. Pandey & Bikash C. Sarkhel

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  1. Dharmendra Kumar
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  2. R. Gopalakrishna
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  3. Ajay P. Singh
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Correspondence to Bikash C. Sarkhel.

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Editor: T. Okamoto

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Kumar, D., Gopalakrishna, R., Singh, A.P. et al. Developmental potency of pre-implant parthenogenetic goat embryos: effect of activation protocols and culture media. In Vitro Cell.Dev.Biol.-Animal 50, 1–6 (2014). https://doi.org/10.1007/s11626-013-9680-7

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  • DOI: https://doi.org/10.1007/s11626-013-9680-7

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