Abstract
Intramuscular long-chain fatty acids (LCFAs) play an important role in energy production and initiation of mitochondrial oxidation of lipids. Herein, we report a natural porcine placenta steroid extract (PPSE) that stimulates transdifferentiation and lipid accumulation in bovine myogenic satellite cells (MSCs). The steroids hormones in PPSE were analyzed using enzyme-linked immunosorbant assay and presence of LCFA was established using gas chromatography. At 70 % confluent growth, cells were treated with PPSE, LCFAs, transdifferentiation cocktail and commercially available steroid hormones. The working concentrations of all chemicals were manipulated similar to PPSE. The cells were observed for morphological changes and subjected to quantitative analysis of lipid deposition on Days 2, 4, and 6 of treatment. PPSE-treated MSCs exclusively transformed into lipid-accumulated adipose-like cells (ALCs). However, myotubes or adipocytes were formed in cells treated with other chemicals. Expression of different genes was studied to ascertain the molecular mechanism involved in ALC formation. CD36, fatty acid binding protein 4, and peroxisome proliferator-activated receptor-gamma were up-regulated. The expression of CD36 was established through immunocyto-chemical analysis. A viability assay was used to confirm the effect of PPSE on proliferation of MSCs. Hence, a natural steroid extract from porcine was found as a nontoxic mixture, which induces lipid accumulation and transdifferentiation of MSCs to ALCs. From the gene expression studies, it was established that the extract works almost in homogenous manner with other lipid inducers.
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Asakura A.; Komaki M.; Rudnicki M. Muscle satellite cells are multipotential stem cells that exhibit myogenic, osteogenic, and adipogenic differentiation. Differentiation 68: 245–253; 2001.
Banerjee K. K.; Bishayee A.; Chatterjee M. Anti-inflammatory effect of human placental extract: a biochemical mechanistic approach. Rivista Europea per le Scienze Mediche e Farmacologiche, 14: 361–366; 1992.
Beloor J.; Kang H. K.; Moon Y. S.; Serum lipids can convert bovine myogenic satellite cells to adipocytes. Asian-Aust. J. Anim. Sci. 23: 1519–1526 ; 2010.
Campbell S. E.; Tandon N. N.; Woldegiorgis G.; Luiken J. J.; Glatz J. F.; Bonen A. A novel function for fatty acid translocase (FAT)/CD36: involvement in long chain fatty acid transfer into the mitochondria. J. Biol. Chem. 279 : 36235–36241; 2004.
Dyck D. J.; Bonen A. Muscle contraction increases palmitate esterification and oxidation and triacylglycerol oxidation. Am. J. Physiol. Endocrinol. Metab. 275: E888-E896; 1998.
Frank S. L.; Radner H.; Walsh A.; Stollberger R.; Knipping G.; Hoefler G.; Sattler W.; Weinstock P. H.; Breslow J. L.; Zechner R.; Muscle-specific overexpression of lipoproteinlipase causes a severe myopathy characterized by proliferation of mitochondria and peroxisomes in transgenic mice. J. Clin. Invest. 96: 976–986; 1995.
Galtz J. F. C.; Storch J. Unravelling the significance of cellular fatty acid binding proteins, Curr. Opin. Lipidol. 12: 267–274; 2001.
Hammarstedt A.; Smith U.; Thiazolidinediones (PPARγ ligands) increase IRS-1, UCP-2 and C/EBPα expression, but not transdifferentiation, in L6 muscle cells. Diabetologia 46: 48–52; 2003.
Hawke T. J.; Garry D. J. Myogenic satellite cells: physiology to molecular biology. J. Appl. Physiol. 91: 534–551; 2001.
Hu E.; Tontonoz P.; Spiegelman B. M. Trandifferentiation of myoblasts by the adipogenic transcription factor PPAR-γ and C/EBP-α, Proc. Natl. Acad. Sci. USA. 92: 9856–9860; 1995.
Lee D. M.; Bajracharya P.; Lee E. J.; Kim J. E.; Lee H. J.; Chun T.; Kim J.; Cho K. H.; Chang J.; Hong S. K.; Choi I. Effects of gender-specific adult bovine serum on myogenic satellite cell proliferation, differentiation and lipid accumulation. In Vitro Cell Dev Biol Animal 47: 438–444; 2011.
Lee E. J.; Bajracharya P.; Jang E. J.; Chang J. S.; Lee H. J.; Hong S. K.; Choi I. Effect of sex steroid hormones on bovine myogenic satellite cell proliferation, differentiation and lipid accumulation in myotube. Asian-Aust. J. Anim. Sci. 23: 649–658, 2010.
Romijn J. A.; Coyle E. F.; Sidossis L. S.; Gastaldelli A.; Horowitz J. F.; Endert E.; Wolfe R. R. Regulation of endogenous fat and carbohydrate metabolism in relation to exercise intensity and duration. Am. J. Physiol. Endocrinol. Metab. 265: E380-E391; 1993.
Rosen E. D.; Hsu C. H.; Wang X.; Sakai S.; Freeman M. W.; Gonzalez F. J.; Spiegelman B. M. C/EBPα induces adipogenesis through PPARγ: a unified pathway. Genes Dev. 16: 22–26; 2002.
Samulin J.; Berget I.; Lien S.; Sundvold H. Differential gene expression of fatty acid binding proteins during porcine adipogenesis. Comp Biochem Physiol Part B 151: 147–152; 2008.
Sfeir Z.; Ibrahimi A.; Amri E. Regulation of FAT/CD36 gene expression: further evidence in support of a role of the protein in fatty acid binding/transport. Prostagl. Leuko. Essent. Fatty Acids 57:17–21; 1997.
Singh N. K.; Chae H. S.; Hwang I. H.; Yoo Y. M.; Ahn C. N.; Lee S. H.; Lee H. J.; Park H. J.; Chung H. Y. Transdifferentiation of porcine satellite cells to adipoblasts with ciglitizone. J. Anim. Sci. 85: 1126–1135; 2007.
Staiger H.; Staiger K.; Haas C.; Weisser M.; Machicao F.; Haring H. U. Fatty acid-induced differential regulation of the genes encoding peroxisome proliferator-activated receptor-gamma coactivator-1alpha and-1betain human skeletal muscle cells that have been differentiated in vitro. Diabetologia 48: 2115–2118; 2005.
Teboul L.; Gaillard D.; Staccini L.; Inadera H.; Amri Z. E.; Grimaldi P. A. Thiazolidinediones and fatty acid convert myogenic cells into adipose-like cells, J. Biol. Chem. 270: 28183–28187; 1995.
Turner N.; Bruce C. R.; Beale S. M; Hoehn K. L.; So T.; Rolph M. S.; Cooney G. J. Evidenceagainat a role for reduced fatty acid oxidation in lipid induced insulin resistance in rodents. Diabetes 56: 85–92; 2007.
Wu C.; Chang G. ; Chang W. ; Hsu C. ; Chen R. Wound healing effects of porcine placental extracts on rats with thermal injury. Brit. J. Dermatol. 148: 236–245; 2003.
Zhang D.; Lijuan G.; Jingjie L.; Zheng L.; Wang C.; Wang Z.; Liu L.; Mira L.; Sung C. Cow placenta extract promotes murine hair growth through enhancing the insulin-like growth factor-1. Indian J. Dermatol. 56: 14–18; 2011.
Zhang P.; Liu C.; Zhang Y.; Shen P.; Zhang J.; Zhang C. Y. Free fatty acids increase PGC-1alpha expression in isolated rat islets. FEBS Lett. 579: 1446–1452; 2005.
Acknowledgments
This work was supported by a grant from the BioGreen 21 Program (project no. PJ 907099), Rural Development Administration, Republic of Korea. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST; no. 2008-0060-480). All research materials used in this study were provided by the Bovine Genome Resources Bank, Yeungnam University, Gyeongsan, Republic of Korea.
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Editor: T. Okamoto
Eun Ju Lee and Majid Rasool Kamli equally contributed to this work.
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Lee, E.J., Kamli, M.R., Bhat, A.R. et al. Effect of porcine placenta steroid extract on myogenic satellite cell proliferation, transdifferentiation, and lipid accumulation. In Vitro Cell.Dev.Biol.-Animal 48, 326–333 (2012). https://doi.org/10.1007/s11626-012-9512-1
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DOI: https://doi.org/10.1007/s11626-012-9512-1