Abstract
Crustacean neurons, obtained from the cerebral ganglion of the mud crab Scylla paramamosain, were successfully cultured in vitro. They maintained typical morphological characteristics and showed better outgrowth in modified Medium 199 (M199) medium than that in Liebowitz’s L-15 medium. Fetal bovine serum (FBS), muscle extracts, and hemolymph of the mud crab S. paramamosain were added as supplements. Only 20% FBS could promote neuron outgrowth, while muscle extracts and hemolymph of S. paramamosain did not improve neuron outgrowth. For cell dissociation, both collagenase type I and trypsin worked well as determined by initial cell viability and following cell outgrowth potential. More than six kinds of cells with different morphological characteristics were identified in the neuron outgrowth. They were “small cells”, “veilers”, “branchers”, “multipolar cells”, “super-large cell”, and “bipolar cells”. Among all of the cells, bipolar cells were identified for the first time in crustacean neurons culture and they could live longer than other cells. The neurons could grow for more than a week before retraction and eventual degradation.
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The National Natural Science Foundation of China under contract nos. 30300269 and 40776084, and the Program for New Century Excellent Talents in Fujian Province University.
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The experiments comply with the current laws of the country in which they were performed.
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Editor: J. Denry Sato
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Xu, Y., Ye, H., Ma, J. et al. Primary culture and characteristic morphologies of neurons from the cerebral ganglion of the mud crab, Scylla paramamosain . In Vitro Cell.Dev.Biol.-Animal 46, 708–717 (2010). https://doi.org/10.1007/s11626-010-9327-x
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DOI: https://doi.org/10.1007/s11626-010-9327-x