Figures 2, 3, and 5 are reproduced in color.

Figure 2.
figure 1

MCF7 cells were incubated with achatininH (8 μg/ml). After 24 and 48 h the cells showed morphological changes (magnification 20 ×). (a) Control, (b) Cells treated with achatininH for 24 h (c) Cells treated with achatininH for 48 h.

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Figure 3.
figure 2

Electrophoresis of purified achatininH. (a) Lane A, molecular weight markers: 212, myosin ; 116, β-galactosidase ; 97.2, phosphorylase B; 66.4, bovine serum albumin; and 14.3, lysozyme. Lane B, SDS - PAGE without β ME; lane C, SDS-PAGE with β ME. (b) Western blot of achatininH with anti-achatininH raised in rabbits.

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Figure 5.
figure 3

AchatininH binding to MCF7 breast cancer cell line detected by immunofluorescence assay. (a) Control, (b) MCF7 cells treated with achatininH for 48 h (magnification 20 ×) (c) cells treated with achatininH for 48 h (magnification 60 ×).

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