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In vitro host range of the Hz-1 nonoccluded virus in insect cell lines

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Abstract

A total of 13 insect cell lines spanning 4 orders (Lepidoptera, Coleoptera, Diptera, and Homoptera) were tested for their ability to replicate the nonoccluded virus Hz-1. Only the Lepidopteran cell lines supported replication of the virus with TN-CL1 and BCIRL-HZ-AM1 producing the highest titers of 2.4 × 108 tissue culture infective dose (TCID)50/ml and 2.0 × 108 TCID50/ml, respectively. A codling moth cell line (CP-169) was the only Lepidopteran cell line that did not replicate the virus and transfection of this cell line with Hz-1 DNA failed to replicate the virus. Also, transfection with DNA from a recombinant baculovirus carrying the red fluorescent protein gene (AcMNPVhsp70 Red) was not expressed in CP-169 cells. The replication cycle of Hz-1 in BCIRL-HZ-AM1 cells showed that this virus replicated rapidly starting at 16 h postinoculation (p.i.) and reaching a peak titer of 1.0 × 108 TCID50/ml 56 h postinoculation. Hz-1 when compared with several other baculoviruses has the widest in vitro host spectrum.

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Acknowledgement

Mention of trade names or commercial product in the publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. All programs and services of the US Department of Agriculture are offered on a nondiscriminatory basis without regard to race, color, national origin, religion, sex, age marital status, or handicap.

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  1. Agricultural Research Service, Biological Control of Insects Research Laboratory, United States Department of Agriculture, 1503 South Providence Road, Research Park, Columbia, MO, 65203-3535, USA

    Arthur H. McIntosh, James J. Grasela & Carlo M. Ignoffo

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  1. Arthur H. McIntosh
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  2. James J. Grasela
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  3. Carlo M. Ignoffo
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Correspondence to Arthur H. McIntosh.

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Editor: J. Denry Sato

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McIntosh, A.H., Grasela, J.J. & Ignoffo, C.M. In vitro host range of the Hz-1 nonoccluded virus in insect cell lines. In Vitro Cell.Dev.Biol.-Animal 43, 196–201 (2007). https://doi.org/10.1007/s11626-007-9032-6

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  • DOI: https://doi.org/10.1007/s11626-007-9032-6

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