Abstract
Although the baculovirus expression vector system (BEVS) is widely used in the production of recombinant proteins, only a few lepidopteran insect cell lines have been successfully used so far. This study aimed at evaluating the characteristics of an embryonic cell line from the American cockroach Periplaneta americana L., RIRI-PA1, and determining whether it could be used in recombinant protein expression. Wild type Autographa californica multiple nucleopolyhedrovirus (AcMNPV-wt) and green fluorescent protein (GFP)–replicating recombinant baculoviruses (AcMNPV-GFP) were used to infect RIRI-PA1 respectively, demonstrating that RIRI-PA1 cells could be infected by AcMNPV and express recombinant proteins. Within 24 h of infection with AcMNPV-GFP, the GFP expression was higher than that in Sf21 cells. Furthermore, the infection of RIRI-PA1 cells increased gradually (multiplicity of infection, 10) within 24 h, while in Sf21 cells, the infection only began to increase within 48 h. However, after exposure for 96–168 h, the virus progeny and recombinant protein production of RIRI-PA1 cells was lower than those of Sf21 cells. Western blotting revealed that RIRI-PA1 cells could express recombinant GFP, and the protein expression level positively correlated with the multiplicity of infection. In conclusion, this is the first report that a cell line from P. americana has shown susceptibility to infection from a baculovirus and likewise express recombinant protein. Although the yield of recombinant GFP was not as high as that of Sf21 cells, the results nonetheless showed that RIRI-PA1 had an infection rate advantage in the short term (within 24 h of infection), which is of great value for further development and utilization.
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Acknowledgements
We would like to acknowledge the China Center for Type Culture Collection for Sf21 cells, and Dr. Ping Tang from Jiangsu University, China, for AcMNPV-wt.
Funding
This work was supported by grants from the Science and Technology Program of Yunnan Provincial Science and Technology Department (No: 2019FB043).
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This report does not contain any studies with human participants or animals. The research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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Ma, C., Zhang, X., Li, X. et al. An embryonic cell line from the American cockroach Periplaneta americana L. (Blattaria: Blattidae) exhibits susceptibility to AcMNPV. In Vitro Cell.Dev.Biol.-Animal 58, 278–288 (2022). https://doi.org/10.1007/s11626-021-00628-9
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DOI: https://doi.org/10.1007/s11626-021-00628-9