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Differentiation of osteoblasts from mouse embryonic stem cells without generation of embryoid body

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Abstract

Osteoblasts are cells specialized in extracellular matrix production and mineralization. In collaboration with osteoclasts which are bone-resorbing cells, osteoblasts regulate bone homeostasis. The study of osteoblast differentiation from the earliest states of the differentiation can be performed using embryonic stem cells. Embryonic stem cells are pluripotent cells which have the capacity to give rise to all kinds of cells of the body. The main protocol to differentiate embryonic stem cells into osteoblast uses the generation of embryoid body which is a three-dimensional structure mimicking the developing embryo. Recently, it has been shown that human embryonic stem cells have the capacity to differentiate spontaneously into osteoblasts. In this manuscript, we showed that mouse embryonic stem cells have the capacity to differentiate spontaneously into osteoblasts, which can be visualized by the appearance of mineralization nodules and osteogenic markers.

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Acknowledgment

LD is supported by an “Association pour la Recherche sur le Cancer” (ARC) postdoctoral fellowship.

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Authors and Affiliations

  1. INSERM, ERI 7, Nantes, 44035, France

    Laurence Duplomb & Dominique Heymann

  2. Université de Nantes, Nantes Atlantique Universités, Laboratoire de Physiopathologie de la Résorption Osseuse et Thérapie des Tumeurs Osseuses Primitives, EA3822, Nantes, 44035, France

    Laurence Duplomb & Dominique Heymann

  3. INSERM U533, Nantes, 44035, France

    Maylis Dagouassat & Philippe Jourdon

Authors
  1. Laurence Duplomb
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  2. Maylis Dagouassat
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  3. Philippe Jourdon
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  4. Dominique Heymann
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Correspondence to Laurence Duplomb or Dominique Heymann.

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Duplomb, L., Dagouassat, M., Jourdon, P. et al. Differentiation of osteoblasts from mouse embryonic stem cells without generation of embryoid body. In Vitro Cell.Dev.Biol.-Animal 43, 21–24 (2007). https://doi.org/10.1007/s11626-006-9010-4

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  • DOI: https://doi.org/10.1007/s11626-006-9010-4

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