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Self-propagating calciferous particles detected in a human cell line Kasumi-6 (JCRB 1024)

In Vitro Cellular & Developmental Biology - Animal volume 42, pages 13–15 (2006)Cite this article

Summary

Tiny particles were found in the medium in the presence of the human leukemia cell line Kasumi-6. The particles were separated from human cells by filtration and incubated in RPMI1640 supplemented with 10% fetal calf serum at 37 C. The particles increased in number very slowly in the liquid medium but did not reveal any biological activity. Transmission electron microscopy of the particles showed a spheroid or ovoid shape in ultrathin section. No specific polypeptides from the purified particles were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), except for bovine fetuin that adsorbed to the surface of the particles. X-ray diffractometry as well as Fourier transform infrared spectrometry suggested the particles consisted of hydroxyapatite. The mechanism of self-propagation of the hydroxyapatite particles in liquid medium is currently unknown. This type of particle has been overlooked for a long period because it is noncultivable. It will be necessary to examine its biological effects to the cultured cells.

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Affiliations

  1. Department of Veterinary Medicine, Iwate University, 020-8550, Morioka, Iwate, Japan

    Ryo Harasawa

  2. School of Advanced Sciences, The Graduate University for Advanced Studies, 240-0193, Hayama, Kanagawa, Japan

    Hideyuki Tanabe

  3. KohjinBio Co. Ltd, Chiyoda, 350-0214, Sakado, Saitama, Japan

    Miharu Kurematsu

  4. National Institute of Biomedical Innovation, 567-0085, Ibaraki, Osaka, Japan

    Hiroshi Mizusawa

  5. Enrironmental Science Center, The University of Tokyo, Bunkyo-ku, 113-0033, Tokyol, Japan

    Yoshimitsu Suzuki

Authors
  1. Ryo Harasawa
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  2. Hideyuki Tanabe
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  3. Miharu Kurematsu
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  4. Hiroshi Mizusawa
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  5. Yoshimitsu Suzuki
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Correspondence to Ryo Harasawa.

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Harasawa, R., Tanabe, H., Kurematsu, M. et al. Self-propagating calciferous particles detected in a human cell line Kasumi-6 (JCRB 1024). In Vitro Cell.Dev.Biol.-Animal 42, 13–15 (2006). https://doi.org/10.1007/s11626-006-0005-y

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  • DOI: https://doi.org/10.1007/s11626-006-0005-y

Key words

  • contamination
  • fetuin
  • hydroxyapatite
  • Kasumi-6
  • nanobacteria