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GSPT1 Functions as a Tumor Promoter in Human Liver Cancer

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Abstract

Objective

This study analyzed the role of G1 to S phase transition 1 protein (GSPT1) in promoting progression of liver cancer cells.

Methods

A bioinformatics database was used to analyze the expression levels of GSPT1 in liver cancer tissues and the prognosis of patients. Subsequently, Western blotting and quantitative PCR were used to verify the expression levels of GSPT1 between normal hepatocytes and hepatoma cells. We used a CRISPR/Cas9 system to construct knockouts of GSPT1 in HepG2 and HCCLM9 liver cancer cells. The effect of GSPT1 on liver cancer cell migration and invasion was analyzed using flow cytometry, migration, and tumor formation assays.

Results

The Cancer Genome Atlas Liver Hepatocellular Carcinoma dataset indicated that GSPT1 expression was upregulated in liver cancer cell lines, and patients with liver cancer had poor prognosis. Knockout of GSPT1 in cells significantly inhibited tumor proliferation, cell migration, and growth in vivo.

Conclusion

In this study, we found that GSPT1 promotes the migration and invasion of liver cancer cells.

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Acknowledgments

The authors thank AiMi Academic Services (https://www.aimieditor.com) for English language editing and review services.

Author information

Author notes

  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Breast and Thyroid Surgery, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China

    Yi-qing Xi, Jing-bo Gao, Li-jie Yang, Si-rui Huang, Wei Xie & Jing-wei Zhang

  2. Department of Gastrointestinal Surgery, Zhongnan Hospital of Wuhan University, Wuhan, 430071, China

    Xuan-fei Li, Hua-qiao Wang, Xiao-chang Fang & Mao-hui Feng

  3. Hubei Cancer Clinical Study Center, Wuhan University, Wuhan, 430071, China

    Li-hua Xu

  4. Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430071, China

    Zhi Li & Jing Wang

Authors
  1. Yi-qing Xi
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  2. Jing-bo Gao
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  3. Xuan-fei Li
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  4. Li-hua Xu
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  5. Zhi Li
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  6. Li-jie Yang
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  7. Jing Wang
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  8. Hua-qiao Wang
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  9. Xiao-chang Fang
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  10. Si-rui Huang
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  11. Wei Xie
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  12. Mao-hui Feng
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  13. Jing-wei Zhang
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Corresponding authors

Correspondence to Mao-hui Feng or Jing-wei Zhang.

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The authors declare no potential conflicts of interest.

Additional information

This work was supported by grants from the National Natural Science Foundation of China (No. 81770283, No. 82070302 and No. 81902018), Clinical Medical Research Center of Peritoneal Cancer of Wuhan (No. 2015060911020462), Natural Science Foundation of Hubei Province (No. 2019CFB109), Technology and Innovation Seed Found, Zhongnan Hospital of Wuhan University (No. znpy2018004).

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Xi, Yq., Gao, Jb., Li, Xf. et al. GSPT1 Functions as a Tumor Promoter in Human Liver Cancer. CURR MED SCI 43, 104–114 (2023). https://doi.org/10.1007/s11596-022-2665-6

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  • DOI: https://doi.org/10.1007/s11596-022-2665-6

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