Abstract
Objective
Y-Chromosomal short tandem repeat polymorphism (Y-STR) analysis plays an indispensable role in the identification of male individuals, population genetics, and biogeographic research. While profiles of many populations based on Y-STR markers in human genomes are ample, haplotype data for the Wuwei Han are still scarce.
Methods
In this study, 2180 unrelated Wuwei Han male individuals residing in Gansu Province, China were collected and genotyped using the novel Microreader™ 40Y Plus ID system. Phylogenetic relationship reconstructions, multidimensional scaling (MDS), and heatmap analysis were performed based on the genetic distance (Rst) values between our studied population and other populations of the Ymax module in the Y-STR Haplotype Reference Database (YHRD).
Results
A total of 2129 unique haplotypes were obtained, and the haplotype diversity (HD) and discrimination capacity (DC) for the Wuwei Han were 0.9999 and 0.9931, respectively.
Conclusion
Our results demonstrate that the Wuwei Han population had intimate genetic relationships with East Asians, especially the geographically close Han populations. Overall, this Y-Chromosomal assay gives valuable information about paternal lineages in male individual tracking and genealogical database construction.
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References
Purps J, Geppert M, Nagy M, et al. Validation of a combined autosomal/Y-chromosomal STR approach for analyzing typical biological stains in sexual-assault cases. Forensic Sci Int Genet, 2015,19:238–242
Kayser M, Kittler R, Erler A, et al. A comprehensive survey of human Y-chromosomal microsatellites. Am J Hum Genet, 2004,74(6):1183–1197
Roewer L. Y chromosome STR typing in crime casework. Forensic Sci Med Pathol, 2009,5(2):77–84
Liu J, Ming TY, Lang M, et al. Exploitation of a novel slowly mutating Y-STRs set and evaluation of slowly mutating Y-STRs plus Y-SNPs typing strategy in forensic genetics and evolutionary research. Electrophoresis, 2021,42(6):774–785
Zhao Y, Yao X, Li Y, et al. Developmental validation of the Microreader Y Prime Plus ID System: An advanced Y-STR 38-plex system for forensic applications. Sci Justice, 2021,61(3):260–270
Liu Y, Lv M, Bai P, et al. Validation of the Microreader 40Y ID System: a Y-STR multiplex for casework and database samples. Int J Legal Med, 2021,135(1):23–41
Fan G, Pan L, Tang P, et al. Technical note: developmental validation of a novel 41-plex Y-STR system for the direct amplification of reference samples. Int J Legal Med, 2021,135(2):409–419
Willuweit S, Roewer L. The new Y chromosome haplotype reference database. Forensic Sci Int Genet, 2015,15:43–48
Li LY, Yao LL, He Xin, et al. Haplotype diversity and phylogenetic characteristics for Guanzhong Han population from Northwest China via 38 Y-STRs using Yfiler™ Platinum Amplification System. Mol Genet Genomic Med, 2020,8(5):e1187
Lang M, Liu H, Song F, et al. Forensic characteristics and genetic analysis of both 27 Y-STRs and 143 Y-SNPs in Eastern Han Chinese population. Forensic Sci Int Genet, 2019,42(7):e13–e20
Li LY, Wu DQ, Yao LL, et al. Improving DNA Data Capacity: Forensic Parameters and Genetic Structure Analysis of Jinjiang Han Population with the MicroreaderTM Y Prime Plus ID System. Curr Med Sci, 2022,42(2):462–466
Walsh PS, Metzger DA, Higuchi R. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques, 1991,10(4):506–513
Roewer L, Andersen MM, Ballantyne J, et al. DNA commission of the International Society of Forensic Genetics (ISFG): Recommendations on the interpretation of Y-STR results in forensic analysis. Forensic Sci Int Genet, 2020,48(7):102308
Gusmão L, Butler JM, Carracedo A, et al. DNA Commission of the International Society of Forensic Genetics (ISFG): An update of the recommendations on the use of Y-STRs in forensic analysis. Forensic Sci Int Genet, 2016,157(2–3):187–197
Nei M, Tajima F. DNA polymorphism detectable by restriction endonucleases. Genetics, 1981,97(1):145–163
Purps J, Siegert S, Willuweit S, et al. A global analysis of Y-chromosomal haplotype diversity for 23 STR loci. Forensic Sci Int Genet, 2014,12(100):12–23
Meirmans PG. Amova-based clustering of population genetic data. J Hered, 2012,103(5):744–750
Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol, 1987,4:406–425
Wang MG, Wang Z, Zhang YQ, et al. Forensic characteristics and phylogenetic analysis of two Han populations from the southern coastal regions of China using 27 Y-STR loci. Forensic Sci Int Genet, 2017,31:e17–e23
Song F, Xie MK, Xie BW, et al. Genetic diversity and phylogenetic analysis of 29 Y-STR loci in the Tibetan population from Sichuan Province, Southwest China. Int J Legal Med, 2020,134(2):513–516
Acknowledgements
We thank Xin HE for guiding the data analysis.
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This study was supported by grants from the Shaanxi Basic Research Program of Natural Science (No. 2021JQ-392) and the International Cooperation Project of Shaanxi Province (No. 2021KW-47).
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Li, Ly., Fan, Sg., Gong, H. et al. Haplotype and Genetic Analysis of 41 Y-STR Loci in the Wuwei Han Population from Gansu Province, China. CURR MED SCI 43, 184–190 (2023). https://doi.org/10.1007/s11596-022-2664-7
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DOI: https://doi.org/10.1007/s11596-022-2664-7