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Effects of Photodynamic Therapy on Nav1.7 Expression in Spinal Dorsal Root Ganglion Neurons

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Abstract

Objective

The aim of this study was to examine the effects of photodynamic therapy (PDT) on the expression of Nav1.7 in spinal dorsal root ganglion (DRG) neurons.

Methods

The primary DRG neurons from newborn SD rats were cultured. The cells were identified by neuron-specific enolase immunofluorescence staining. DRG neurons were divided into four groups: control group, photosensitizer group, laser group, and PDT group. The cell viability was detected by a cell counting kit-8 (CCK8) assay. qRT-PCR and Western blotting were used to determine the mRNA and protein expression levels of Nav1.7 in DRG neurons.

Results

The purity of the cultured primary DRG neurons was greater than 90%. Compared with the control group, no significant change was found in the cell viability of the photosensitizer group, while the viability in the laser group and the PDT group was significantly reduced. The mRNA and protein expression levels of Nav1.7 were significantly greater in the laser group and the PDT group than in the control group. At the same time, the mRNA and protein expression levels of Nav1.7 were greater in the laser group than in the PDT group.

Conclusion

Both laser and PDT could upregulate the expression of Nav1.7 in DRG neurons, and the promoting effect might be related to the pain induced by clinical treatment. This study provides a research basis for the use of laser and PDT to treat pain. A better understanding of the relationship between Nav1.7 and PDT can help clinicians better manage PDT-related pain.

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Author information

Authors and Affiliations

  1. Medical School of Chinese PLA, Beijing, 100853, China

    Dong-xu Zou & Hong Cai

  2. Department of Dermatology, Air Force Medical Center, PLA, Beijing, 100142, China

    Dong-xu Zou, Xiao-zhen Li & Hong Cai

Authors
  1. Dong-xu Zou
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  2. Xiao-zhen Li
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  3. Hong Cai
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Corresponding author

Correspondence to Hong Cai.

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The authors declare that they have no conflicts of interest.

Additional information

This project was financially supported by Natural Science Foundation of Beijing Municipality (No. 7202200), Central Military Commission Health Care Project (No. 20BJZ13), and Air Force Equipment Scientific Research Project (No. KJ20201A050226).

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Zou, Dx., Li, Xz. & Cai, H. Effects of Photodynamic Therapy on Nav1.7 Expression in Spinal Dorsal Root Ganglion Neurons. CURR MED SCI 42, 1267–1272 (2022). https://doi.org/10.1007/s11596-022-2640-2

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  • DOI: https://doi.org/10.1007/s11596-022-2640-2

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