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Enhanced biocompatibility and osseointegration of calcium titanate coating on titanium screws in rabbit femur

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Summary

This study aimed to examine the biocompatibility of calcium titanate (CaTiO3) coating prepared by a simplified technique in an attempt to assess the potential of CaTiO3 coating as an alternative to current implant coating materials. CaTiO3-coated titanium screws were implanted with hydroxyapatite (HA)-coated or uncoated titanium screws into medial and lateral femoral condyles of 48 New Zealand white rabbits. Imaging, histomorphometric and biomechanical analyses were employed to evaluate the osseointegration and biocompatibility 12 weeks after the implantation. Histology and scanning electron microscopy revealed that bone tissues surrounding the screws coated with CaTiO3 were fully regenerated and they were also well integrated with the screws. An interfacial fibrous membrane layer, which was found in the HA coating group, was not noticeable between the bone tissues and CaTiO3-coated screws. X-ray imaging analysis showed in the CaTiO3 coating group, there was a dense and tight binding between implants and the bone tissues; no radiation translucent zone was found surrounding the implants as well as no detachment of the coating and femoral condyle fracture. In contrast, uncoated screws exhibited a fibrous membrane layer, as evidenced by the detection of a radiation translucent zone between the implants and the bone tissues. Additionally, biomechanical testing revealed that the binding strength of CaTiO3 coating with bone tissues was significantly higher than that of uncoated titanium screws, and was comparable to that of HA coating. The study demonstrated that CaTiO3 coating in situ to titanium screws possesses great biocompatibility and osseointegration comparable to HA coating.

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Authors and Affiliations

  1. Department of Orthopedics, Third Xiangya Hospital, Central South University, Changsha, 410013, China

    Zi-li Wang  (王梓力), Rong-zhen He  (何榕真), Xu Cao  (曹 旭), Jin-shen He  (何金深), Han-song Xia  (夏晗松), Chi Liang  (梁 驰) & Song Wu  (吴 松)

  2. Department of Orthopaedics, Leping People’s Hospital, Leping, 333399, China

    Bin Tu  (涂 斌)

  3. Department of Orthopaedics, Ningxiang People’s Hospital, Ningxiang, 410600, China

    Min Zou  (邹 敏)

  4. College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, China

    Zhen-jun Wu  (吴振军)

  5. Department of Anatomy and Neurobiology, School of Basic Medical Science, Central South University, Changsha, 410013, China

    Kun Xiong  (熊 鲲)

Authors
  1. Zi-li Wang  (王梓力)
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  2. Rong-zhen He  (何榕真)
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  3. Bin Tu  (涂 斌)
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  4. Xu Cao  (曹 旭)
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  5. Jin-shen He  (何金深)
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  6. Han-song Xia  (夏晗松)
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  7. Chi Liang  (梁 驰)
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  8. Min Zou  (邹 敏)
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  9. Song Wu  (吴 松)
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  10. Zhen-jun Wu  (吴振军)
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  11. Kun Xiong  (熊 鲲)
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Corresponding authors

Correspondence to Song Wu  (吴 松), Zhen-jun Wu  (吴振军) or Kun Xiong  (熊 鲲).

Additional information

This project was supported by the National Natural Science Foundation of China (Nos. 81572150, 81571939), the Natural Science Foundation of Hunan Province (No. 2015JJ2187), and the Wu Jie-Ping Medical Foundation of the Minister of Health of China (No. 320675014118).

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Wang, Zl., He, Rz., Tu, B. et al. Enhanced biocompatibility and osseointegration of calcium titanate coating on titanium screws in rabbit femur. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 37, 362–370 (2017). https://doi.org/10.1007/s11596-017-1741-9

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  • DOI: https://doi.org/10.1007/s11596-017-1741-9

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