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Distinct expression of chemokine-like factor 1 in synovium of osteoarthritis, rheumatoid arthritis and ankylosing spondylitis

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Summary

Chemokine-like factor 1 (CKLF1) is a newly cloned chemotactic cytokine with CCR4 being its functional receptor. Recent evidence demonstrates a role of CKLF1 in arthritis. The aim of this study was to quantify the expression of CKLF1 as well as assess the correlation between CKLF1 and plasma acute-phase markers. Synovium was obtained from 16 osteoarthritis (OA), 15 rheumatoid arthritis (RA) and 10 ankylosing spondylitis (AS) patients undergoing total joint arthroplasty, with other 11 patients treated for meniscal tears during sport accidents serving as normal controls. Levels of CKLF1 and CCR4 mRNA were detected by qRT-PCR, and the expression of CKLF1 was investigated by immunohistochemistry staining, subsequently analyzed with semiquantitative scores. Plasma acute-phase markers of inflammation were determined by ELISA. CKLF1 was found with a particularly up-regulated expression in synovim from AS and RA patients, and CCR4 mRNA levels increased in RA patients, not in OA or AS patients. Elevated levels of plasma markers of inflammation including CRP, ESR and Ddimer were observed in RA. Further, significantly positive correlations between relative expression levels of CKLF1 and CRP/ESR in RA patients and a positive correlation between CKLF1 and ESR in AS patients were found. There was no detectable correlation between CKLF1 and plasma D-dimer. This study confirms an increased but different level of CKLF1 in RA, OA and AS patients, all significantly higher than that in controls. Additionally, the significant positive correlations between CKLF1 levels and CRP/ESR in RA and between CKLF1 and ESR suggest that CKLF1 might contribute to the inflammation state and clinical symptoms in these rheumatic diseases. Further studies are required to investigate the utility of targeting specific CKLF1 for symptom control or disease modification in RA and AS.

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Authors and Affiliations

  1. Arthritis Clinical and Research Center, Peking University People’s Hospital, Beijing, 100044, China

    Ke Tao  (陶 可), Xu Tang  (唐 旭), Bin Wang  (王 斌), Ru-jun Li  (李儒军), Bao-qing Zhang  (张宝庆), Jian-hao Lin  (林剑) & Hu Li  (李 虎)

  2. Peking University Health Science Center, Beijing, 100083, China

    Ke Tao  (陶 可), Bin Wang  (王 斌) & Bao-qing Zhang  (张宝庆)

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  1. Ke Tao  (陶 可)
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  2. Xu Tang  (唐 旭)
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  3. Bin Wang  (王 斌)
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  4. Ru-jun Li  (李儒军)
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  5. Bao-qing Zhang  (张宝庆)
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  6. Jian-hao Lin  (林剑)
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  7. Hu Li  (李 虎)
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Correspondence to Hu Li  (李 虎).

Additional information

This project was supported by National Natural Science Foundation of China (No. 81441056 and 81541134).

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Tao, K., Tang, X., Wang, B. et al. Distinct expression of chemokine-like factor 1 in synovium of osteoarthritis, rheumatoid arthritis and ankylosing spondylitis. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 36, 70–76 (2016). https://doi.org/10.1007/s11596-016-1544-4

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  • DOI: https://doi.org/10.1007/s11596-016-1544-4

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