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Effects of IL-17 on expression of GRO-α and IL-8 in fibroblasts from nasal polyps

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Summary

Recent studies indicated that interleukin (IL)-17, growth-related oncogene (GRO)-α and IL-8 play an important role in the pathogenesis of nasal polyps. However, the effects of the increased amount of IL-17 and the production of GRO-α and IL-8 in human nasal polyp fibroblasts are not completely understood. This study aimed to determine the effects of the increased IL-17 on the changes of GRO-α and IL-8 expression in human nasal polyp fibroblasts and further investigate the mechanism of neutrophil infiltration in nasal polyps. Nasal polyp fibroblasts were isolated from six cases of human nasal polyps, and the cells were stimulated with five different concentrations of IL-17. Real-time fluorescence quantitative polymerase chain reaction (RT-PCR) was used to detect the mRNA expression of GRO-α and IL-8. The mRNA of GRO-α and IL-8 was expressed in unstimulated controls and remarkably increased by stimulation with IL-17. Moreover, the levels of GRO-α and IL-8 produced by fibroblasts were increased gradually with the increases in IL-17 concentrations. The present study showed that nasal fibroblasts can produce GRO-α and IL-8, and their production is remarkably enhanced by IL-17 stimulation, thereby clarifying the mechanism of the IL-17 mediated neutrophil infiltration in nasal polyps. These findings might provide a rationale for using IL-17 inhibitors as a treatment for nasal inflammatory diseases such as nasal polyps.

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Authors and Affiliations

  1. Institute of Otorhinolaryngology, Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Yong-zhi Niu  (牛永芝), Guo-qing Gong  (龚国清), Shan Chen  (陈 删), Jian-jun Chen  (陈建军), Wei-jia Kong  (孔维佳) & Yan-jun Wang  (王彦君)

Authors
  1. Yong-zhi Niu  (牛永芝)
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  2. Guo-qing Gong  (龚国清)
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  3. Shan Chen  (陈 删)
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  4. Jian-jun Chen  (陈建军)
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  5. Wei-jia Kong  (孔维佳)
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  6. Yan-jun Wang  (王彦君)
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Corresponding authors

Correspondence to Wei-jia Kong  (孔维佳) or Yan-jun Wang  (王彦君).

Additional information

This project was supported by grants from Research and National Promotion of Early Detection, Standardized Diagnosis and Treatment, and Preventive Strategy for Major Otology and Rhinologic Diseases (No. 201202005), Wu Jieping Medical Foundation (No. LC1345), and Foundation of Hubei Province Key Laboratory of Molecular Imaging (No. 02.03.2013-64).

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Niu, Yz., Gong, Gq., Chen, S. et al. Effects of IL-17 on expression of GRO-α and IL-8 in fibroblasts from nasal polyps. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 34, 591–595 (2014). https://doi.org/10.1007/s11596-014-1321-1

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  • DOI: https://doi.org/10.1007/s11596-014-1321-1

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