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Effects of Chinese Fructus Mume formula and its separated prescription extract on insulin resistance in type 2 diabetic rats

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Summary

The effect of Fructus Mume formula and its separated prescription extract on insulin resistance in type 2 diabetic rats was investigated. The rat model of type 2 diabetes was established by feeding on a high-fat diet for 8 weeks and by subsequently intravenous injection of small doses of streptozotocin. Rats in treatment groups, including the Fructus Mume formula treatment group (FM), the cold property herbs of Fructus Mume formula treatment group (CFM), the warm property herbs of Fructus Mume formula treatment group (WFM), were administrated with Fructus Mume formula and its separated prescription extract by gavage, while the rats in diabetic model group (DM) and metformin group (MET) were given by gavage with normal saline and metformin correspondingly. The body weight before and after treatment was measured, and the oral glucose tolerance test (OGTT) and the insulin release test (IRT) were performed. The homeostasis model assessment-insulin resistance index (HOMA-IR) was calculated. The protein and mRNA expression levels of Insr, β-arrestin-2, Irs-1 and Glut-4 in the liver, skeletal muscle and fat tissues were detected by using Western blotting and RT-PCR respectively. The results demonstrated that, as compared with DM group, OGTT, IRT (0 h, 1 h) levels and HOMR-IR in treatment groups were all reduced, meanwhile their protein and mRNA expression levels of Insr, Irs-1 and Glut-4 in the liver, skeletal muscle and fat tissues were obviously increased, and their protein and mRNA expression levels of β-arrestin-2 in the liver and skeletal muscle tissues were also markedly increased. It was suggested that the Fructus Mume formula and its separated prescription extracts could effectively improve insulin resistance in type 2 diabetic rats, which might be related to the up-regulated expression of Insr, Irs-1 and Glut-4 in the liver, skeletal muscle and fat tissues, and β-arrestin-2 in the liver and skeletal muscle tissues.

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Authors and Affiliations

  1. Institute of Integrative Traditional Chinese & Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Jing-bin Li  (李井彬), Li-jun Xu  (徐丽君), Hui Dong  (董 慧), Zhao-yi Huang  (黄诏宜), Yan Zhao  (赵 炎), Guang Chen  (陈 广) & Fu-er Lu  (陆付耳)

Authors
  1. Jing-bin Li  (李井彬)
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  2. Li-jun Xu  (徐丽君)
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  3. Hui Dong  (董 慧)
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  4. Zhao-yi Huang  (黄诏宜)
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  5. Yan Zhao  (赵 炎)
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  6. Guang Chen  (陈 广)
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  7. Fu-er Lu  (陆付耳)
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Corresponding authors

Correspondence to Guang Chen  (陈 广) or Fu-er Lu  (陆付耳).

Additional information

This project was supported by grants from the National Natural Science Foundation of China (No. 81273683) and the Foundation of Hubei Provincial Health Department (No. 2012Z-Y04).

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Li, Jb., Xu, Lj., Dong, H. et al. Effects of Chinese Fructus Mume formula and its separated prescription extract on insulin resistance in type 2 diabetic rats. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 33, 877–885 (2013). https://doi.org/10.1007/s11596-013-1215-7

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  • DOI: https://doi.org/10.1007/s11596-013-1215-7

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