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Molecular mechanisms underlying the time-dependent autophagy and apoptosis induced by nutrient depletion in multiple myeloma: a pilot study

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Summary

This study explored the molecular mechanisms underlying the time-dependent autophagy and apoptosis induced by nutrient depletion in human multiple myeloma cell line RPMI8226 cells. RT-PCR and qRT-PCR were used to evaluate the transcriptional levels of Deptor, JNK1, JNK2, JNK3, Raf-1, p53, p21 and NFκB1 at 0, 6, 12, 18, 24 and 48 h after nutrient depletion in RPMI8226 cells. We found that transcriptional levels of Deptor were increased time-dependently at 0, 6, 12 and 18 h, and then decreased. Its alternation was consistent with autophagy. Transcriptional levels of Raf-1, JNK1, JNK2, p53 and p21 were increased time-dependently at 0, 6, 12, 18, 24 and 48 h accompanying with the increase of apoptosis. Transcriptional levels of NFκB1 at 6, 12, 18, 24 and 48 h were decreased as compared with 0 h. It was suggested that all the studied signaling molecules were involved in cellular response to nutrient depletion in RPMI8226 cells. Deptor contributed to autophagy in this process. Raf-1/JNK /p53/p21 pathway may be involved in apoptosis, and NFκB1 may play a possible role in inhibiting apoptosis. It remained to be studied whether Deptor was involved in both autophagy and apoptosis.

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Authors and Affiliations

  1. Department of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Yuan Liu  (刘 媛), Yan Chen  (陈 燕), Lu Wen  (文 璐) & Guohui Cui  (崔国惠)

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  1. Yuan Liu  (刘 媛)
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  2. Yan Chen  (陈 燕)
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  3. Lu Wen  (文 璐)
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  4. Guohui Cui  (崔国惠)
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Corresponding author

Correspondence to Guohui Cui  (崔国惠).

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The authors contributed equally to this work.

This project was supported by a grant from National Natural Science Foundation of China (No. 30871036).

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Liu, Y., Chen, Y., Wen, L. et al. Molecular mechanisms underlying the time-dependent autophagy and apoptosis induced by nutrient depletion in multiple myeloma: a pilot study. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 32, 1–8 (2012). https://doi.org/10.1007/s11596-012-0001-2

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  • DOI: https://doi.org/10.1007/s11596-012-0001-2

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