Skip to main content
SpringerLink
Log in

Correlation of vascular endothelial growth factor and CD105-microvascular density in primary pterygium

  • Published:
Journal of Huazhong University of Science and Technology [Medical Sciences] Aims and scope Submit manuscript

Summary

The relationship between the expression of vascular endothelial growth factor (VEGF) and microvascular density (MVD) marked by CD105 (CD105-MVD), and that between CD105-MVD and the clinicopathological characteristics of primary pterygium were investigated. The streptavidin-biotin complex (SABC) immunohistochemical staining in paraffin-embedded tissues was used to detect the expression of VEGF in 23 cases of primary pterygia and 7 normal conjunctival specimens. The antibody against CD105 was used to display vascular endothelial cells, and MVD was examined by counting the CD105-positive vascular endothelial cells. The correlations of VEGF and CD105-MVD, and those of CD105-MVD and clinicopathological data were analyzed by using SPSS 12.0. The expression of VEGF was significantly increased in epithelia (P=0.000), endothelia and stroma cells (P=0.005) in primary pterygia as compared with normal conjunctivae. The CD105-MVD in pterygia (mean 19.22±6.68) was higher than that in normal conjunctivae (mean 4.00±2.15, P=0.000). MVD in pterygia was significantly associated with the Tan classification (P=0.000) and the VEGF expression level in the stroma (P=0.020), but not with sex (P=0.61), age (P=0.150) or the VEGF expression level in the epithelia (P=0.518). Our results suggest that over-expression of VEGF and high CD105-MVD in primary pterygium may contribute to the progression by increasing angiogenesis and growth of primary pterygium.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Dushku N, Reid TW. Immunohistochemical evidence that human pterygia originate from an invasion of vimentin-expressing altered limbal epithelial basal cells. Curr Eye Res, 1994,13(7):473–481

    Article  PubMed  CAS  Google Scholar 

  2. Clear AS, Chirambo MC, Hutt MS. Solar keratosis, pterygium, and squamous cell carcinoma of the conjunctiva in Malawi. Br J Ophthalmol, 1979,63(2):102–109

    Article  PubMed  CAS  Google Scholar 

  3. Peiretti E, Dessi S, Putzolu M, et al. Hyperexpression of low-density lipoprotein receptors and hydroxy-methylgl utaryl-coenzyme A-reductase in human pinguecula and primary pterygium. Invest Ophthalmol Vis Sci, 2004, 45(11):3982–3985

    Article  PubMed  Google Scholar 

  4. Lee DH, Cho HJ, Kim JT, et al. Expression of vascular endothelial growth factor and inducible nitric oxide synthase in pterygia. Cornea, 2001,20(7):738–742

    Article  PubMed  CAS  Google Scholar 

  5. Jin J, Guan M, Sima J, et al. Decreased pigment epithelium-derived factor and increased vascular endothelial growth factor levels in pterygia. Cornea, 2003,22(5):473–477

    Article  PubMed  Google Scholar 

  6. van Setten G, Aspiotis M, Blalock TD, et al. Connective tissue growth factor in pterygium: simultaneous presence with vascular endothelial growth factor-possible contributing factor to conjunctival scarring. Graefes Arch Clin Exp Ophthalmol, 2003,241(2):135–139

    Article  PubMed  Google Scholar 

  7. Gebhardt M, Mentlein R, Schaudig U, et al. Differential expression of vascular endothelial growth factor implies the limbal origin of pterygia. Ophthalmology, 2005, 112(6):1023–1030

    Article  PubMed  Google Scholar 

  8. Kria L, Ohira A, Amemiya T. Growth factors in cultured pterygium fibroblasts: immunohistochemical and ELISA analysis. Graefes Arch Clin Exp Ophthalmol, 1998, 236(9):702–708

    Article  PubMed  CAS  Google Scholar 

  9. Kria L, Ohira A, Amemiya T. Immunohistochemical localization of basic fibroblast growth factor, platelet derived growth factor, transforming growth factor-beta and tumor necrosis factor-alpha in the pterygium. Acta Histochem, 1996,98(2):195–201

    PubMed  CAS  Google Scholar 

  10. Leung DW, Cachianes G, Kuang WJ, et al. Vascular endothelial growth factor is a secreted angiogenic mitogen. Science, 1989,246(4935):1306–1309

    Article  PubMed  CAS  Google Scholar 

  11. Senger DR, Galli SJ, Dvorak AM, et al. Tumor cells secrete a vascular permeability factor that promotes accumulation of ascites fluid. Science, 1983,219(4587): 983–985

    Article  PubMed  CAS  Google Scholar 

  12. Ferrara N, Henzel WJ. Pituitary follicular cells secrete a novel heparin-binding growth factor specific for vascular endothelial cells. Biochem Biophys Res Commun, 1989,161(2):851–858

    Article  PubMed  CAS  Google Scholar 

  13. Unemori EN, Ferrara N, Bauer EA, et al. Vascular endothelial growth factor induces interstitial collagenase expression in human endothelial cells. J Cell Physiol, 1992,153(3):557–562

    Article  PubMed  CAS  Google Scholar 

  14. Lindgren M, Johansson M, Sandstrom J, et al. VEGF and tPA co-expressed in malignant glioma. Acta Oncol, 1997,36(6):615–618

    Article  PubMed  CAS  Google Scholar 

  15. Duff SE, Li C, Garland JM, et al. CD105 is important for angiogenesis: evidence and potential applications. FASEB J, 2003,17(9):984–992

    Article  PubMed  CAS  Google Scholar 

  16. Tan DT, Chee SP, Dear KB, et al. Effect of pterygium morphology on pterygium recurrence in a controlled trial comparing conjunctival autografting with bare sclera excision. Arch Ophthalmol, 1997,115(10):1235–1240

    PubMed  CAS  Google Scholar 

  17. Weidner N, Carroll PR, Flax J, et al. Tumor angiogenesis correlates with metastasis in invasive prostate carcinoma. Am J Pathol, 1993,143(2):401–409

    PubMed  CAS  Google Scholar 

  18. Aspiotis M, Tsanou E, Gorezis S, et al. Angiogenesis in pterygium: study of microvessel density, vascular endothelial growth factor, and thrombospondin-1. Eye (Lond), 2007,21(8):1095–1101

    CAS  Google Scholar 

  19. Marcovich AL, Morad Y, Sandbank J, et al. Angiogenesis in pterygium: morphometric and immunohistochemical study. Curr Eye Res, 2002,25(1):17–22

    Article  PubMed  Google Scholar 

  20. Saad RS, Liu YL, Nathan G, et al. Endoglin (CD105) and vascular endothelial growth factor as prognostic markers in colorectal cancer. Mod Pathol, 2004,17(2):197–203

    Article  PubMed  CAS  Google Scholar 

  21. Kumar S, Ghellal A, Li C, et al. Breast carcinoma: vascular density determined using CD105 antibody correlates with tumor prognosis. Cancer Res, 1999,59(4):856–861

    PubMed  CAS  Google Scholar 

  22. Wikstrom P, Lissbrant IF, Stattin P, et al. Endoglin (CD105) is expressed on immature blood vessels and is a marker for survival in prostate cancer. Prostate, 2002,51(4):268–275

    Article  PubMed  CAS  Google Scholar 

  23. Ding Y, Zhang M. The expression of CD105 and VEGF in corneal neovascularization of rats. Acta Med Univ Sci Technol Huazhong (Chinese), 2010,39(2):221–224

    CAS  Google Scholar 

  24. Seifert P, Sekundo W. Capillaries in the epithelium of pterygium. Br J Ophthalmol, 1998,82(1):77–81

    Article  PubMed  CAS  Google Scholar 

  25. Solomon A, Li DQ, Lee SB, et al. Regulation of collagenase, stromelysin, and urokinase-type plasminogen activator in primary pterygium body fibroblasts by inflammatory cytokines. Invest Ophthalmol Vis Sci, 2000,41(8): 2154–2163

    PubMed  CAS  Google Scholar 

  26. Di Girolamo N, Wakefield D, Coroneo MT. UVB-mediated induction of cytokines and growth factors in pterygium epithelial cells involves cell surface receptors and intracellular signaling. Invest Ophthalmol Vis Sci, 2006,47(6):2430–2437

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Jie Zhang  (张 洁), Mingchang Zhang  (张明昌), Xiaoqing Li  (李晓青), Tian Zheng  (郑 恬), Ge Mu  (穆 歌), Wei Liu  (刘 伟), Huatao Xie  (谢华桃) & Xin Liu  (刘 欣)

Authors
  1. Jie Zhang  (张 洁)
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mingchang Zhang  (张明昌)
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiaoqing Li  (李晓青)
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tian Zheng  (郑 恬)
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ge Mu  (穆 歌)
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Wei Liu  (刘 伟)
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Huatao Xie  (谢华桃)
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Xin Liu  (刘 欣)
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mingchang Zhang  (张明昌).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, J., Zhang, M., Li, X. et al. Correlation of vascular endothelial growth factor and CD105-microvascular density in primary pterygium. J. Huazhong Univ. Sci. Technol. [Med. Sci.] 31, 560–564 (2011). https://doi.org/10.1007/s11596-011-0490-4

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11596-011-0490-4

Key words

Search

Navigation