Summary
The effects of the cyclin E expression levels on chemotherapeutic sensitivity of breast cancer cell line were explored. After the cyclin E expression was knockdown in MDA-MB-435 by RNA interference, FACS analysis and SA-β-gal staining were used to evaluate the response sensitivity of breast cancer cells to DNA damage drugs (adriamycin, etc.). Adriamycin could induce G1 arrest in cyclin E knockdown MDA-MB-435 breast cell line and increase the percentage of cell senescence in cyclin E knockdown MDA-MB-435 cells. It was suggested that cyclin E knockdown could increase the chemotherapeutic sensitivity of breast cancer cells to DNA damage drugs.
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This project was supported by a grant from National Natural Sciences Foundation of China (No. 30400432).
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Chen, J., Wang, G. Cyclin E expression and chemotherapeutic sensitivity in breast cancer cells. J. Huazhong Univ. Sc. Technol. 26, 565–566 (2006). https://doi.org/10.1007/s11596-006-0521-8
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DOI: https://doi.org/10.1007/s11596-006-0521-8