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P53 regulation of leukemia cells with the blockage of MDM2 by antisense oligonucleotides

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Summary

The changes of expression and function of MDM2 and P53 by MDM2 specific antisense oligonucleotides were investigated in HL60 cells. Cells were divided into control group, AS group (MDM2 specific antisense oligonucleotides group), cisplatin group, and combined treatment group. FCM analysis and Western blot and RT-PCR were used to estimate apoptosis and the expression of MDM2 and P53. Our results showed that the transfection of MDM2 specific antisense oligonucleotides obviously inhibited MDM2 expression (P<0.01) and increased the expression of P53 (P<0.05). Apoptosis rate were reduced by MDM2 specific antisense oligonucleitides and cisplatin (P<0.01). It is concluded that MDM2 specific antisense oligonucletides can inhibit the expression of MDM2, induce the expression of P53 and increase the apoptosis of leukemia cells after chemotherapy.

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References

  1. Vassilve L T, Vu B T, Graves B et al. In vivo activation of the P53 Pathway by small-molecule antagonist of MDM2. Science, 2004,30: 844–848

    Article  Google Scholar 

  2. Zhang Z, Li M, Wang H et al. Antisense therapy targeting MDM2 oncogene in Prostate cancer: Effects on Proliferation, apoptosis, multiple gene expression and chemotherapy. Proc Natl Acad Sci USA, 2003,100: 11636–11641

    Article  PubMed  CAS  Google Scholar 

  3. Wu X., Bayle J H, Olson D et al. The P53-MDM2 autoregulatory feedback loop. Genes Dev. 1993, 7: 1126–1132.

    PubMed  CAS  Google Scholar 

  4. Kastan M B, Onyekwere O, Sidransky D et al. Participation of P53 Protein in the cellular response to DNA damage. Cancer Res, 1991,51: 6304–6311

    PubMed  CAS  Google Scholar 

  5. Mu Z, Hachmen P, Agrawal S et al. Antisense MDM2 oligonucleotides restore the apoptotic response of Prostate cancer cells to androgen deprivation. The Prostate 2004,60: 187–196

    Article  PubMed  CAS  Google Scholar 

  6. Peng Y, Chen L, Li C et al. Stabilization of the MDM2 oncoprotein by mutant P53. J Biol Chem. 2001,276: 6874–6878

    Article  PubMed  CAS  Google Scholar 

  7. Shimizu H and Hupp T R. Intrasteric regulation of MDM2. TRENDS Biol Sci, 2003,28: 346–349

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Internal Emergency, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Fang Minghao  (房明浩)

  2. Department of Chemotherapy and Radiation, Zhongnan Hospital, Wuhan University, Wuhan, 430071, China

    Ji Xuemei  (纪学梅)

  3. Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Tang Yi  (汤屹) & Liu Wenli  (刘文励)

Authors
  1. Fang Minghao  (房明浩)
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  2. Ji Xuemei  (纪学梅)
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  3. Tang Yi  (汤屹)
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  4. Liu Wenli  (刘文励)
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Additional information

FANG Minhao, born in 1972, Doctor in Charge

This project was supported by a grant from the Natural Science Fund of Hubei Province (No. 2005ABA150).

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Fang, M., Ji, X., Tang, Y. et al. P53 regulation of leukemia cells with the blockage of MDM2 by antisense oligonucleotides. J. Huazhong Univ. Sc. Technol. 26, 414–416 (2006). https://doi.org/10.1007/s11596-006-0409-7

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  • DOI: https://doi.org/10.1007/s11596-006-0409-7

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