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Physiochemical and biological properties of modified collagen sponge from porcine skin

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Abstract

The aim of the present study was to compare one-step method to EDC/NHS crosslinking (EDC/NHS group) and one-step simultaneous method to EDC/NHS crosslinking and heparin immobilization (EDC/NHS- Heparin group) in improving physiochemical and biological properties of native collagen sponge (Control group). Modified collagen sponge overcome the disadvantages of native collagen sponge. IR spectra suggest the change of the functional groups. DSC data indicate that the stability of caloric transformation in EDC/NHS group is slightly higher than that of EDC/NHS-Heparin group. The crosslinking degree, stability against enzymes, stability in morphologically and biomechanical properties of EDC/NHS-Heparin group are higher than those of EDC/NHS group, whereas, the water-binding capacity in EDC/NHS-Heparin group is lower than that of EDC/NHS group. HUVECs in EDC/NHS-Heparin group scaffold proliferate fast, migrate well and distribute uniformly. One-step simultaneous method gains the better effects in above aspects, heparinized collagen matrices increase in angiogenic potential and suit for defect repairing and tissue engineering.

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Authors and Affiliations

  1. Institute of Medical Equipment, Academy of Military Medical Sciences, Tianjin, 300161, China

    Yuanyuan Xu  (许媛媛), Jimin Wu  (武继民), Jing Guan, Xizheng Zhang, Zhihong Li, Pengfei Wang, Ruixin Li, Yong Guo, Bo Ning & Shujie Huang

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  1. Yuanyuan Xu  (许媛媛)
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  2. Jimin Wu  (武继民)
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  3. Jing Guan
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  4. Xizheng Zhang
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  5. Zhihong Li
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  6. Pengfei Wang
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  7. Ruixin Li
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  8. Yong Guo
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  9. Bo Ning
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  10. Shujie Huang
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Correspondence to Jimin Wu  (武继民).

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Funded by the National Natural Science Foundation of China (10832012) and the Natural Science Foudation of Tianjin city(08JCYBJC03400)

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Xu, Y., Wu, J., Guan, J. et al. Physiochemical and biological properties of modified collagen sponge from porcine skin. J. Wuhan Univ. Technol.-Mat. Sci. Edit. 24, 619–626 (2009). https://doi.org/10.1007/s11595-009-4619-2

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  • DOI: https://doi.org/10.1007/s11595-009-4619-2

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