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Application of an ionic liquid-functionalized Mg2Al layered double hydroxide for the electrochemical myoglobin biosensor

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Abstract

In this paper, an ionic liquid 1-carboxyl-methyl-3-methylimidazolium tetrafluoroborate (CMMIMBF4)-functionalized Mg2Al layered double hydroxide (LDH) was synthesized and further used for the immobilization of myoglobin (Mb) on the surface of a carbon ionic liquid electrode to get a new electrochemical biosensor. Ultraviolet–visible and Fourier transform–infrared spectroscopies confirmed that Mb in the CMMIMBF4-LDH remained its native secondary structure, which was attributed to the biocompatibility of the materials used. On the cyclic voltammograms, a pair of well-defined redox peaks appeared, indicating that direct electron transfer of Mb was realized in the modified electrode. The formal peak potential was calculated as −0.209 V (vs. SCE), which was the typical characteristics of the Mb heme Fe(III)/Fe(II) redox couples. The fabricated Mb sensor exhibited good electrocatalytic activity to the reduction of trichloroacetic acid in the range from 1.0 to 17.0 mmol L−1 with the detection limit as 0.344 mmol L−1 (3σ), and the apparent Michaelis–Menten constant was calculated as 13.5 mmol L−1. Thus, the ionic liquid-functionalized LDH exhibited the potential application in the electrochemical sensor for redox proteins.

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Acknowledgments

We are grateful to the financial support of the Natural Science Foundation (No. 51363008), the Natural Science Foundation of Jiangxi Province (20122BAB206012), the Open Foundation from the Key Laboratory of Active Material and Modern Analysis Technology, SOA (MBSMAT-2012-07 and MBSMAT-2013-01), and the Youth Foundation of the Jiangxi Provincial Education Department (GJJ11222).

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Authors and Affiliations

  1. Jiangxi Key Laboratory of Surface Engineering, Jiangxi Science and Technology Normal University, Jiangxi, 330013, People’s Republic of China

    Jin Lou & Chengxiang Ruan

  2. College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, People’s Republic of China

    Yongxi Lu, Tianrong Zhan, Yaqing Guo & Wei Sun

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  1. Jin Lou
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  2. Yongxi Lu
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  3. Tianrong Zhan
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  4. Yaqing Guo
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  5. Wei Sun
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Correspondence to Wei Sun.

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Lou, J., Lu, Y., Zhan, T. et al. Application of an ionic liquid-functionalized Mg2Al layered double hydroxide for the electrochemical myoglobin biosensor. Ionics 20, 1471–1479 (2014). https://doi.org/10.1007/s11581-014-1088-1

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  • DOI: https://doi.org/10.1007/s11581-014-1088-1

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