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Mycological Progress

, Volume 18, Issue 1–2, pp 135–146 | Cite as

Studies on the biologically active secondary metabolites of the new spider parasitic fungus Gibellula gamsii

  • Wilawan Kuephadungphan
  • Allan Patrick G. Macabeo
  • Janet Jennifer Luangsa-ard
  • Kanoksri Tasanathai
  • Donnaya Thanakitpipattana
  • Souwalak Phongpaichit
  • Kamila Yuyama
  • Marc StadlerEmail author
Open Access
Original Article
  • 2.9k Downloads

Abstract

Numerous gatherings of a new species of the genus Gibellula, closely resembling the monotypic, neotropical G. mirabilis were encountered in Thailand. The taxon was cultured successfully although no in vitro sporulation was observed. The new species, Gibellula gamsii, could be distinguished from closely related other Gibellula species on the basis of morphological features and phylogenetic inferences recruiting concatenated sequences of five DNA loci including ITS, LSU, RPB1, RPB2, and EF1-α. The secondary metabolites of G. gamsii, strain BCC47868, were studied concurrently after preparative separation of the crude extract by preparative high-performance liquid chromatography (HPLC). Two new 1,3-disubstituted β-carboline alkaloids, for which we propose the trivial names, gibellamines A (1) and B (2), were isolated. The chemical structures of these compounds were elucidated by interpretation of spectral data, generated by nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS). The alkaloid 1 also exhibited moderate anti-biofilm activity against Staphylococcus aureus.

Keywords

Bioprospecting Cordycipitaceae Invertebrate-associated fungi New species 

Introduction

The genus Gibellula Cavara 1894 (Cordycipitaceae) comprises parasites of spiders that produce cylindrical synnemata arising from host cadavers, exclusively attached to the underside of living leaves, conidiophores with slender apices, obovoid vesicles bearing a number of broadly obovoid metulae, and narrowly clavate phialides bearing conidia found (Mains 1950; Samson and Evans 1992). The genus was established to accommodate G. pulchra and revised by Petch (1932) and Mains (1950). Since then, numerous further species have been proposed (Humber and Rombach 1987; Kobayasi and Shimizu 1982; Samson and Evans 1992; Tzean et al. 1998), and the genus now comprises ca. 30 taxa.

The generic concept of Gibellula mainly relies on its morphological features, while the phylogenetic relationships among species remain poorly understood due to lack of molecular data. Attempts to sequence the ITS rDNA barcode of Gibellula species collected in Thailand were made (BIOTEC fungal barcoding project, unpublished), while Johnson et al. (2009) constructed a multi-gene phylogeny of Torrubiella by using SSU, LSU, EF1-α, RPB1, and RPB2 sequences with only few links to Gibellula. Aside from the fact that access to holotype specimens has been limited, no ex-type strains are available for most Gibellula species. In general, only a few attempts to generate DNA sequence data of Gibellula have so far been made, mostly of the ITS region, and especially poorly represented are type materials as only the ex-type culture of G. clavulifera var. alba was sequenced previously (Kepler et al. 2012; Spatafora et al. 2007), which is known to provide insufficient resolution for species discrimination of Sordariomycetes and many other Ascomycota (Helaly et al. 2018; Hongsanan et al. 2018; Wendt et al. 2018).

In the course of surveying invertebrate-pathogenic fungi and searching for biologically active fungal metabolites, a fungus resembling the Latin American Gibellula mirabilis was collected from various localities throughout Thailand. Its salient morphological traits are its clavate brush-like synnemata with golden brown sterile tips. Samson and Evans (1992) failed to culture the holotype specimen of this fungus as no conidia germinated, and the species has not been reported ever since.

Herein, we report the morphology of a new Gibellula species and describe its phylogenetic placement based on concatenated sequences of five loci (ITS, LSU, RPB1, RPB2, and EF1-α). We also isolate and characterize two secondary metabolites from cultures of G. gamsii, which proved to constitute unprecedented natural products.

Materials and methods

Collection of material and isolation of strains

Specimens were collected from rain forests in various localities throughout Thailand. Spider cadavers colonized by fungi attached on the underside of living leaves were carefully removed from leaves and transported to the laboratory for isolation performed immediately on the same day as described by Kuephadungphan et al. (2014). For microscopic examination, lactophenol cotton blue was used for staining. The specimens were deposited in the BIOTEC Bangkok Herbarium (BBH), while the living cultures were deposited in the BIOTEC Culture Collection (BCC) and the Thailand Biological Resource Center (TBRC).

Molecular phylogenetic analyses

Fungal isolates were grown on potato dextrose agar (PDA; Laboratorios CONDA, Madrid, Spain) at 24 °C for 1–4 weeks, depending on the growth rate of individual strains. After incubation, about 60 mg of fungal mycelium was harvested and subsequently transferred to a 1.5mL screw cap reaction tube with 6–10 sterile 1.4mm ceramic beads (Precellys®). The DNA extraction was performed using the EZ-10 Spin Column Genomic DNA Minipreps Kit (BIO BASIC INC., Canada), following the manufacturer’s instructions. The PCR amplification of five nuclear DNA regions including internal transcribed spacer (ITS), the nuclear ribosomal large subunit DNA (LSU), translation elongation factor 1-alpha (EF1-α), and the largest and second largest subunits of RNA polymerase II (RPB1 and RPB2) were done in 25 μL volume consisting of 12.5 μL of 2× JumpStart Taq ReadyMix (20 mM Tris-HCl, pH 8.3, 100 mM KCl, 3 mM MgCl2, 0.002% gelatin, 0.4 mM of each dNTP (dATP, dCTP, dGTP, TTP), stabilizers, 0.1 unit/μL Taq DNA Polymerase, JumpStart Taq antibody, Sigma-Aldrich, Deisenhofen, Germany), 0.5 μL of each primer, 9.5 μL of sterile milli-Q water, and 2 μL of DNA template. Primers ITS1F (Gardes and Bruns 1993) and ITS4 (White et al. 1990) were used for ITS, 983F and 2218R (Rehner and Buckley 2005) for EF1-α, LR5 or LR7 (Vilgalys and Hester 1990) and LROR (Bunyard et al. 1994) for LSU, RPB1-Ac and RPB1-Cr (Murata et al. 2014) for RPB1, and fRPB2-5F and fRPB2-7cR (Liu et al. 1999) for RPB2. The amplification was performed with the programs as described by Luangsa-ard et al. (2005), Sung et al. (2001), Chaverri and Samuels (2003), Luangsuphabool et al. (2016), and Sung et al. (2007b) for ITS, LSU, EF1-α, RPB1, and RPB2, respectively. The PCR products were purified using an EZ-10 Spin Column PCR purification Kit (BIO BASIC INC., Canada) according to the manufacturer’s instructions. The sequencing was conducted in both forward and reverse directions by the in-house sequencing service of the Helmholtz-Centre of Infection Research, Germany, and Macrogen (South Korea).

Each sequence read was checked for ambiguous bases and assembled using BioEdit v.7.2.5 (Hall 1999; Hall et al. 2011). The alignment was conducted using MAFFT 7.017 with G-INS as algorithm and default settings for gap open and gap extension penalties (Katoh and Toh 2008), and manual adjustments were made in BioEdit. The multigene alignment was subsequently created by concatenation of all regions in Geneious® 7.1.9 (http://www.geneious.com, Kearse et al. 2012). The best fitting substitution model for the multigene alignment was determined by using jModeltest 2.1.7 (Darriba et al. 2012; Guindon and Gascuel 2003; Posada 2008). The phylogenetic relationships were inferred using maximum likelihood (ML) with GTR+GAMMA+I in RAxML 7.2.8 (Stamatakis 2006) and the rapid bootstrap analysis algorithm. Relative support for the branches was obtained from bootstrap analysis with 1000 replicates.

Instrumentation for spectral measurements

High-resolution electrospray ionization mass spectrometry (HRESIMS) was conducted, using an Agilent 1200 series HPLC system in combination with an electrospray ionization time-of-flight mass spectrometric (ESI-TOF-MS; Maxis, Bruker, Bremen, Germany) module [column 2.1 × 50 mm, 1.7 μm, C18 Acquity UPLC BEH (Waters, Eschborn, Germany), solvent A: 95% 5 mM ammonium acetate buffer (pH 5.5, adjusted with 1 M acetic acid) with 5% acetonitrile; solvent B: 95% acetonitrile with 5% 5 mM ammonium acetate buffer]. Nuclear magnetic resonance (NMR) spectra were recorded on Bruker Avance III 500 MHz spectrometer with a BBFO (plus) SmartProbe (1H 500 MHz, 13C 125 MHz) and a BrukerAvance III 700 MHz spectrometer with a 5-mm TCI cryoprobe (1H 700 MHz, 13C 175 MHz). Chemical shifts δ were referenced to the solvents: chloroform-d (1H, δ = 7.27 ppm;13C, δ = 77.0 ppm) and MeOH-d4 (1H, δ = 3.31 ppm; 13C, δ = 49.15 ppm). UV spectra were recorded using a Shimadzu (Duisburg, Germany) UV-vis spectrophotometer UV-2450.

Isolation and structure elucidation of the secondary metabolites

Gibellula gamsii strain BCC47868 was grown on yeast-malt-glucose (YMG) medium (Rupcic et al. 2018). Seven mycelial plugs (1 × 1 cm2) from actively growing colonies were transferred into 20 × 500 mL Erlenmeyer flasks containing 200 mL YMG medium and incubated on shaker operating at 120 rpm, 24 °C for 5 weeks. The culture filtrate (broth) obtained after separation from the fungal mycelia was extracted with ethyl acetate (4 L) to give a pale yellowish powder (170 mg). A portion of the crude broth extract was dissolved in methanol and chemically profiled on Agilent 1260 UHPLC Infinity Systems (Santa Clara, CA, USA) with diode array detector and Waters C18 Acquity UPLC BEH column (2.1 × 50 mm, 1.7 μm) using a previously described gradient elution condition (Noumeur et al. 2017) combined with ion trap MS (amaZon speed, Bruker, Bremen, Germany), and HR-ESIMS spectra on a time-of-flight (TOF) MS (Maxis, Bruker, Germany) to optimize chromatographic purification conditions. Isolation of compounds was achieved with Agilent 1100 series HPLC system (Agilent Technologies, Wilmington, DE, USA) with diode array detection. A reverse-phase C18 column (Kromasil 250 × 20 mm, 7 μm; MZ Analysentechnik, Mainz, Germany) was used as stationary phase. The mobile phase was composed of acid-free deionized water (Milli-Q, Millipore, Schwalbach, Germany: solvent A) and acid-free acetonitrile (ACN: solvent B). Separation of compounds from the crude extract (150 mg) was carried out in 2× injection in accord with the following gradients: linear gradient from 30 to 45% solvent B in 20 min, followed by linear gradient to 70% solvent B in 20 min and linear gradient to 100% solvent B in 10 min, and finally, isocratic conditions at 100% for 10 min, with a flow rate of 20 mL/min. UV detection was carried out at 210, 280, and 354 nm. Fractions were collected and pooled according to the observed peaks. Compounds 1 (18.2 mg) and 2 (1.0 mg) were afforded at retention times, (Rt) = 14–15 and 32–33 min, respectively.

Gibellamine A, 1-(3-(hydroxymethyl)-9H-pyrido[3,4-b]indol-1-yl)ethan-1-one (1): yellow powder; UV (MeOH) (log ε) 217 nm (6.14, λmax), 257 (5.67), 284 (5.79), 306 (5.41), 381 (5.36) nm; 1H NMR (500 MHz, MeOH-d4) see Table 2, 13C NMR (125 MHz, CDCl3) see Table 2; HRESIMS m/z 241.0970 [M + H]+ (calcd for C14H13N2O2, 241.0972)

Gibellamine B, (1-acetyl-9H-pyrido[3,4-b]indol-3-yl)methyl acetate (2): yellow syrup; UV (MeOH) (log ε) 217 nm (6.05, λmax), 253 (5.62), 283 (5.71), 307 (5.31), 381 (5.26) nm; 1H NMR (700 MHz, CDCl3) see Table 2, 13C NMR (175 MHz, CDCl3) see Table 2; HRESIMS m/z 283.1115 [M + H]+ (calcd for C16H15N2O3, 283.1109)

Biological activity screening

The minimum inhibitory concentrations (MICs) of alkaloids 1 and 2 against Bacillus subtilis DSM10, Escherichia coli DSM498, Candida tenuis MUCL29892, and Mucor plumbeus MUCL49355 were determined using a broth microdilution method previously described by Kuephadungphan et al. (2017). Their nematicidal activity against Caenorhabditis elegans and anti-biofilm activity against Staphylococcus aureus DSM1104 (ATCC25923) as well as Pseudomonas aeruginosa PA14 were also investigated using the protocols according to Helaly et al. (2017) and Chepkirui et al. (2018). The cytotoxicity assay was performed on mouse fibroblast (L929), HeLa (KB3.1), human breast adenocarcinoma (MCF-7), human prostate cancer (PC-3), squamous carcinoma (A431), human lung carcinoma(A549), and ovarian carcinoma (SKOV-3) cell lines using a 5-day MTT assay and IC50 values were expressed in micrograms per milliliter (Chepkirui et al. 2017).

Results and discussion

Taxonomy

Gibellula gamsii Kuephadungphan, Tasanathai & Luangsa-ard, sp. nov. Fig. 1.
Fig. 1

Gibellula gamsii: a, b synnema arising from the host, c part of synnema with golden brown sterile tip, d conidiophores showing conidial head, e part of conidiophore showing rough walls, f conidiophore head bearing conidia, g conidia, and h colonies obverse and reverse on PDA at 25 °C after 40 days. Scale bars: d 50 μm, e, f 20 μm, g 10 μm

Mycobank MB 825141

Etymology: in honor of the late Prof. Walter Gams, for his outstanding contribution to our knowledge of anamorphic hypocrealean fungi

Diagnosis: differs from Gibellula mirabilis, to which it is morphologically similar, in lacking a granulomanus-like conidial state, in mostly forming single synnemata and in having longer, more variable conidiophores with larger conidial heads and smaller conidia. Differs from G. alata and G. unica in synnemata that terminate in a non-sporulating, wing-like structure and by forming numerous synnemata.

Holotype: Thailand, Trang, Khao Banthat Wildlife Sanctuary, on Araneida on unidentified dicot leaf, 18 Sep. 2007, K. Tasanathai; B. Thongnuch (BBH31372, ex-type culture BCC27968)

Habitat: occurring on unidentified, small spiders found on the underside of living leaves

Description: mycelium white to yellowish-white, completely covering spider body. Synnemata yellowish-white to pale yellow, solitary or in groups of three, 5–15 mm long, with clavate, brush-like fertile area, 3–4 × 0.8–1.2 mm; narrowing to a slender, short stipe (ca. 1–2 mm) and terminating in a wing-like, rounded, yellow to golden brown sterile tip that consists of a delicate network of loose, hyaline, anastomosing hyphae (0.5–1 mm long). Conidiophores numerous arising from surface hyphae, scattered, verrucose, hyaline, (10–) 24–57 (− 91) × (2–) 3.5–5.5 (− 6) μm, narrowing to a slender apex and terminating in a swollen vesicle. Conidial head spherical, (31) 3742.5 (48) diam. Vesicles ellipsoidal to globose, (6) 79 (10) × (5) 6.58.5 (10), bearing a number of metulae on the upper portion. Metulae ellipsoidal to obovoidal, (3.5) 68.5 (10) × (3.5) 46 (8) μm. Phialides mostly oblong-elliptical, (5.5) 6.58.5 (11) × (2) 1.53 μm. Conidia fusiform to fusiform-elliptical, smooth-walled, hyaline, (3) 3.55 (5.5) × (1) 1.52.5 (3) μm. Granulomanus-like synanamorph not observed

Culture characteristics: isolates on PDA slow growing, attaining a diam of 1 cm in 3–4 weeks at 25 °C. Colonies white to yellowish-white; reverse pale yellow to brown at the center, or entirely brown with age. No sporulation observed

Additional specimens examined: Thailand, Trang, Khao Banthat Wildlife Sanctuary, on Araneida on unidentified leaves, 18 Sep. 2007, K. Tasanathai; B. Thongnuch (BBH27524, living culture BCC27970). Kamphaeng Phet, Khlong Lan National Park, on Araneida on unidentified leaves, 6 Nov. 2007, K. Tasanathai; S. Mongkolsamrit; P. Srikitikulchai; B. Thongnuch; R. Ridkaew; A. Khonsanit; W. Chaygate (BBH22756, living culture BCC28798; BBH23098, living culture BCC29228; BBH27573, living culture BCC28797; BBH28376, living culture BCC30449; BBH28378, living culture BCC30396; BBH28379, living culture BCC30397; BBH28372, living culture BCC28801). Chiang Mai, Huai Nam Dang National Park, on Araneida, underside of unidentified living leaf, 5 Jul. 2008, K. Tasanathai; S. Mongkolsamrit; B. Thongnuch; P. Srikitikulchai; A. Khonsanit (BBH27583). Nakhon Ratchasima, Khao Yai National Park, on Araneida, underside of unidentified living leaf, 5 Feb. 2009, K. Tasanathai; S. Mongkolsamrit; J. J. Luangsa-ard; W. Chaygate, P. Puyngain; R. Ridkaew; T. Chohmee (BBH27242); 16 Aug. 2009, K. Tasanathai; S. Mongkolsamrit; T. Chohmee; R. Ridkaew (BBH27250, living culture BCC39922; BBH27251; BBH27252); 12 Sep. 2009, K. Tasanathai; P. Srikitikulchai; S. Mongkolsamrit; T. Chohmee; R. Ridkaew (BBH27258, living culture BCC39024); 13 Sep. 2009, K. Tasanathai; P. Srikitikulchai; S. Mongkolsamrit; T. Chohmee; R. Ridkaew (BBH27259, living culture BCC39038); 6 Oct. 2009, K. Tasanathai; P. Srikitikulchai; S. Mongkolsamrit; T. Chohmee, R. Ridkaew, P. Puyngain; M. Sudhadham (BBH27274, living culure BCC39953); 7 Oct. 2009, K. Tasanathai; P. Srikitikulchai; S. Mongkolsamrit; T. Chohmee; R. Ridkaew; P. Puyngain; M. Sudhadham (BBH27275, living culture BCC39966); 10 Nov. 2009, K. Tasanathai; S. Mongkolsamrit; T. Chohmee; R. Ridkaew; M. Sudhadham; A. Khonsanit (BBH27281, living culture BCC40856); 12 Nov. 2009, K. Tasanathai; S. Mongkolsamrit; T. Chohmee; R. Ridkaew; M. Sudhadham; A. Khonsanit (BBH27286); 7 Jan. 2010, K. Tasanathai; P. Srikitikulchai; S. Mongkolsamrit; T. Chohmee; R. Ridkaew; M. Sudhadham; A. Khonsanit (BBH27351, living culture BCC40909); 3 Mar. 2010, K. Tasanathai; P. Srikitikulchai; S. Mongkolsamrit; T. Chohmee A. Khonsanit (BBH28510, living culture BCC42026); 20 May 2010, A. Khonsanit; R. Somnuk (BBH28560); 31 May 2010, K. Tasanathai, P. Srikitikulchai; S. Mongkolsamrit; T. Chohmee; A. Khonsanit; R. Somnuk; K. Sansatchnon (BBH29333). Songkhla, Ton-Nga-Chang Wildlife Sanctuary, on Araneida, underside of unidentified living leaf, 20 Jun. 2009, W. Kuephadungphan; W. Phetmanee (EPF034, living culture BCC47868)

Notes: Gibellula gamsii shows morphological similarities with G. mirabilis (Samson and Evans 1992). Both produce clavate, brush-like synnemata that terminate into a sterile tip. Most specimens of G. gamsii produced a single synnema per specimen; only in some specimens were the synnemata found in pairs or in groups of three. Samson and Evans (1992) reported synnemata in groups of two for G. mirabilis. Moreover, in G. gamsii, the length of conidiophores varied from (10–) 24–57 (− 91) μm, while for G. mirabilis, they are up to 80 μm long. Conidial heads were larger while conidia were smaller in G. gamsii [(31) 3742.5 (48) μm diam, (3) 3.55 (5.5) × (1) 1.52.5(3) μm] compared to those of G. mirabilis [25–40 μm diam, 5–7 × 2–3.5 μm]. Furthermore, the granulomanus-like synanamorph reported for G. mirabilis could not be seen in any of the Thai specimens at any developmental stage. The significance of a granulomanus-like conidial state for species discrimination of Gibellula is unclear and requires study on larger numbers of specimens and strains. It is known, however, that Gibellula and Granulomanus naturally appear either concurrently or independently on the same spider hosts (Samson et al. 1989). Several species of Gibellula has been reported from Asia, such as G. alata from Sri Lanka (Petch 1932) and G. unica from Taiwan (Tzean et al. 1997). Although the conidial sizes of G. gamsii fall into the range of those reported for G. alata (4–9 × 2–4 μm) and G. unica (4–6.8 × 1.6–2.2 μm), G. gamsii can be easily distinguished from both species. G. alata typically produces wing-like structures at the swollen tip of synnema while G. unica forms numerous cylindric synnemata as well as granulomanus-like synanamorph. Some Asian Gibellula species such as G. clavulifera var. major produce long conidia (7.1–12 × 2.4–4 μm) and penicillium-like conidiophores (Tzean et al. 1997).

Gibellula was previously distinguished from Pseudogibellula by its specialized growth requirements as it could not be established in culture (Samson and Evans 1973). Samson and Evans (1992) pointed out that the majority of Gibellula species are very hard to isolate although they previously succeeded in growing G. clavulifera on mealworm agar. Tzean et al. (1997) failed to culture G. clavulifera, G. leiopus, G. pulchra, and G. unica collected in Taiwan from single or mass conidia or mycelia on diverse artificial media. In our study, cultures of G. gamsii were successfully isolated from conidia even on the standard medium PDA. This property may also turn out to be characteristic for the new species.

Molecular phylogeny

Thirty-one fungal taxa were included in the analyses, 10 of which resulted from our own work and 21 datasets were taken from GenBank (Table 1). The calculated MAFFT alignments consisted of 4108 characters, 664, 860, 718, 937, and 869 of which were derived from the ITS, LSU, RPB1, RPB2, and EF1-α datasets, respectively.
Table 1

List of taxa used in the current phylogenetic study. The type strains are indicated with a superscript T (T)

Species

Strain number

GenBank accession numbers

Reference

ITS

LSU

RPB1

RPB2

EF1-α

Akanthomyces aculeatus

HUA186145T

 

MF416520

  

MF416465

Kepler et al. 2017

Akanthomyces sabanensis

ANDES-F1014

KC633245

KC633248

  

KC875221

Chiriví-Salomón et al. 2015

Akanthomyces sabanensis

ANDES-F1024T

KC633232

KC875225

 

KC633249

KC633266

Chiriví-Salomón et al. 2015

Beauveria bassiana

ARSEF7518

HQ880762

 

HQ880834

HQ880906

HQ880975

Rehner et al. 2011

Beauveria bassiana

ARSEF1564T

HQ880761

 

HQ880833

HQ880905

HQ880974

Rehner et al. 2011

Blackwellomyces cardinalis

OSC93610

JN049843

AY184963

EF469088

EF469106

EF469059

Kepler et al. 2012; Sung & Spatafora 2004; Sung et al. 2007a

Blackwellomyces cardinalis

OSC93609T

 

AY184962

DQ522370

DQ522422

DQ522325

Spatafora et al. 2007; Sung & Spatafora 2004

Cordyceps fumosorosea

CBS244.31

AY624182

MF416557

MF416660

MF416454

MF416503

Kepler et al. 2017; Luangsa-ard et al. 2005

Cordyceps fumosorosea

CBS107.10T

AY624184

MF416556

MF416659

MF416453

MF416502

Kepler et al. 2017; Luangsa-ard et al. 2005

Gibellula cf. alba

NHJ11679

  

EU369054

 

EU369016

Johnson et al. 2009

Gibellula clavulifera var. alba

ARSEF1915T

JN049837

DQ518777

DQ522408

DQ522467

DQ522360

Kepler et al. 2012; Spatafora et al. 2007

Gibellula leiopus

BCC16025

 

MF416548

MF416649

 

MF416492

Kepler et al. 2017

Gibellula longispora

NHJ12014

  

EU369055

EU369075

EU369017

Johnson et al. 2009

Gibellula pulchra

NHJ10808

 

EU369035

EU369056

EU369076

EU369018

Johnson et al. 2009

Gibellula gamsii

BCC25798

MH152532

MH152542

MH152550

 

MH152563

This study

Gibellula gamsii

BCC27968

MH152529

MH152539

MH152547

 

MH152560

This study

Gibellula gamsii

BCC27970

MH152530

MH152540

MH152548

 

MH152561

This study

Gibellula gamsii

BCC28797

MH152531

MH152541

MH152549

MH152557

MH152562

This study

Gibellula gamsii

BCC29228

MH152533

MH152543

MH152551

MH152558

MH152564

This study

Gibellula gamsii

BCC30396

MH152535

 

MH152553

 

MH152566

This study

Gibellula gamsii

BCC30397

MH152536

 

MH152554

 

MH152567

This study

Gibellula gamsii

BCC30449

MH152534

MH152544

MH152552

MH152559

MH152565

This study

Gibellula gamsii

BCC42026

MH152537

MH152545

MH152555

 

MH152568

This study

Gibellula gamsii

BCC47868

JX192720

MH152538

MH152546

MH152556

JX192817

This study

Hevansia cinerea

BCC2191

GQ250000

GQ249971

  

GQ250029

Ridkaew et al. unpublished data

Hevansia cinerea

NHJ3510

  

EU369048

EU369070

EU369009

Johnson et al. 2009

Hevansia novoguineensis

NHJ4314

  

EU369051

EU369071

EU369012

Johnson et al. 2009

Hevansia novoguineensis

NHJ11923

 

EU369032

EU369052

EU369072

EU369013

Johnson et al. 2009

Hevansia websteri

BCC23860

GQ250009

GQ249979

  

GQ250030

Ridkaew et al. unpublished data

Engyodontium aranearum

CBS309.85

AJ292391

AF339526

DQ522387

DQ522439

DQ522341

Spatafora et al. 2007; Sung et al. 2001; Zare et al. 2000

Engyodontium aranearum

CBS658.80

LC092897

LC092916

   

Tsang et al. 2016

The attempt to include the β-tubulin coding gene (TUB) which was generated using standard primers introduced by Liu et al. (1999) (RPB2-5F and RPB2-7cR) in the analyses was made. The concatenated multiple gene dataset (ITS, LSU, RPB1, RPB2, EF1-α, and TUB) was created and the phylogenetic tree was then generated according to the protocol described above. Interestingly, the multigene tree which is provided in the Supplementary Material showed very long branches at some certain nodes as well as uncorrelated topologies compared with those previously described by Chiriví-Salomón et al. (2015), D’Alessandro et al. (2013), and Kepler et al. (2017). Therefore, TUB was excluded from the dataset but it is included in the Supplementary Material.

The multigene tree (Fig. 2) comprised seven different genera belonging to the family Cordycipitaceae including Akanthomyces, Beauveria, Blackwellomyces, Cordyceps, Gibellula, Hevansia, and outgroup Engyodontium. The analyses showed the genera segregated corresponding to the most recent phylogenetic classification of the Cordycipitaceae (Mongkolsamrit et al. 2018). Gibellula has been inferred as a monophyletic group in previous phylogenetic studies (Johnson et al. 2009; Kepler et al. 2017). A Gibellula clade composed of G. clavulifera cf. alba, G. cf. alba, G. leiopus, G. longispora, G. pulchra, and G. gamsii was strongly supported (99%). Sequences of all isolates of the new species also formed a strongly supported (100% BS value) species clade and were inferred as the phylogenetic sister of G. leiopus.
Fig. 2

RAxML tree based on the concatenated five gene datasets (ITS, LSU, RPB1, RPB2, and EF1-α) showing the relationship among Gibellula and related genera. Bootstrap proportions ≥ 50% are provided above corresponding nodes; nodes with a BS of 100% are shown as thick lines. The ex-type strains are marked with a superscript T (T) and the isolates encountered in this study are highlighted

Isolation and structure elucidation of alkaloids 1 and 2

G. gamsii was fermented using YMG medium and the fermentation broth was extracted with ethyl acetate and analyzed by HPLC-(DAD)-UV-TOFMS. Two sets of metabolites with characteristic UV absorption bands were observed upon analysis of chromatograms (Fig. 3) and spectroscopic data (UV and MS). Subsequent HPLC-(DAD)-UV-guided fractionation led to the isolation of two new compounds, 1 and 2, belonging to β-carboline class of alkaloids.
Fig. 3

HPLC-(DAD)-UV chromatogram of the crude (YMG broth) ethyl acetate extract of Gibellula gamsii

Gibellamine A (1) was isolated as pale yellow solid. Its molecular formula was determined as C14H12N2O2 by HRESIMS, requiring 10 degrees of unsaturation. The UV spectrum of 1 exhibited characteristic absorption bands at 217, 284, and 381 nm, diagnostic of a β-carboline chromophore (Wang et al. 2008). The 1H and 13C spectroscopic data of 1 (Table 1) displayed signals assignable to one methyl (C-11), one oxygenated methylene (C-12), five aromatic methines, six unsaturated quaternary carbons, and one carbonyl functionality (C-10). The 1H–1H COSY and HSQC NMR experiments suggested the presence of 1,2-disubstituted benzenoid substructure (C-5–C-8) (Fig. 4). The methyl singlet at δH 2.82 (H3–11) showed HMBC correlation to the carbonyl at δC 203.7 (C-10), suggesting the presence of an acetyl group. Additional HMBC correlation of H3–11/C-1 located the acetyl group at C-1 to form the 1-acetyl-β-carboline moiety in 1. The attachment of the acetyl carbonyl group (C-10) at C-1 with a beta-nitrogen atom in the pyridine sub-structure positioned the chemical shift of H3–11 to a lower field. In the 1H NMR spectrum of 1, a downfield singlet was readily discernible at δH 8.38, indicating the proton was localized at H-4, corresponding to the peri-position of the β-carboline skeleton. The HMBC correlations from H-4 to C-9a and C-4a supported this assumption (Fig. 4). The singlet proton chemical shifts at H-4 and H2–12 also showed HMBC correlations to an oxygenated methylene moiety at δC 66.6 (C-12) and δC 117.5, respectively, suggesting C-12 was connected to C-3. Therefore, a 1,3-disubsituted β-carboline core structure was established. Hence, the gross structure of 1 was constructed and determined as 1-(3-(hydroxymethyl)-9H-pyrido[3,4-b]indol-1-yl)ethan-1-one with the assigned trivial name, gibellamine A.
Fig. 4

COSY and selected HMBC correlations in 1 (a) and 2 (b)

Gibellamine B (2) was obtained as a yellow syrup. It exhibited a prominent pseudomolecular ion peak at m/z 283.1115 [M + H]+ in HRESIMS, indicating the molecular formula C16H14N2O3, which is 42 mass units greater than that of 1 and corresponds to the addition of an acetyl group. The UV, 1H, and 13C NMR spectroscopic data of 2 strongly resembled those of 1 (Table 1), except additional signals corresponding to the acetyl methyl at δH 2.21 (H3-2′)/δC 21.1 (C-2′) and carboxyl group at δC 170.9 (C-1′) were observed in 2. These changes are supported by HMBC correlations noted between H3-2′ with C-1′ and C-12 (δC 67.6) (Fig. 4). Therefore, compound 2 was identified as (1-acetyl-9H-pyrido[3,4-b]indol-3-yl)methyl acetate and named gibellamine B.

The COSY, HSQC-DEPT, HMBC, 1H, and 13C NMR spectra of compounds 12 are available as Supplementary Material.

While a number of β-carboline alkaloid derivatives have been reported from different natural sources, most 1,3-disubstituted carbazole alkaloids bearing an acetyl group at C-1 and a carboxylic acid derivative functionality at C-3 such as 3 (Fig. 5) have been exclusively reported in many alkaloid-containing plant genera (Cao et al. 2012; Wen et al. 2014; Zhang et al. 2015) and bacteria (Huang et al. 2011; Kornsakulkarn et al. 2013). In the fungal kingdom, isolates of marine origin produce harman-type β-carboline alkaloids such as derivatives observed in a Capnodium sp. (He et al. 2016) and Neosartorya pseudofischeri (currently valid name: Aspergillus fischeri; Lan et al. 2016). Interestingly, harman alkaloids (4) (Fig. 5) have also been identified from the insect-associated fungus Ophiocordyceps sinensis (Yang et al. 2011) and from the hypocrealean species, Trichoderma virens (Liu et al. 2012). The new compounds are the first examples of C-16-reduced derivatives compared to other naturally occurring alkaloids of similar type which features a carboxyl group instead of an oxymethylene moiety.
Fig. 5

Structure of β-carboline alkaloids from Gibellula gamsii

Determination of biological activities

A number of 1,3-disubstituted β-carboline alkaloids have been reported to exhibit a wide range of biological activities including anti-acetylcholinesterase (Zhou et al. 2015), anti-allergenic (Sun et al. 2004), anti-bacterial (Wang et al. 2012), anti-cancer (Tian et al. 2012), anti-inflammatory (Chen et al. 2010), anti-malaria (Huang et al. 2011), molluscicidal (Adewunmi et al. 1989), and neurochemical-modulating (Cain et al. 1982) activities. Thus, 1 and 2 were evaluated for their anti-microbial, anti-biofilm, cytotoxic, and nematicidal activities. Weak to insignificant antimicrobial activity was exhibited by both compounds against yeasts, filamentous fungi, and Gram-positive and Gram-negative bacteria. The alkaloid 1 significantly inhibited biofilm formation in Staphylococcus aureus (ATCC 25923) with an MIC value of 62.5 μg/mL.

The cytotoxic activities (Table 2) were rather weak, but a broader range of cytotoxic effects in comparison to compound 1 was observed for gibellamine B (2) against human lung carcinoma A549, mouse fibroblast L929, and squamous carcinoma A431 cell-lines, with IC50 values of 13, 16, and 24 μg/mL, respectively. Weak cytotoxicity was observed for the major alkaloid gibellamine A (1) against L929 with IC50 value of 28 μg/mL. In addition, both alkaloids did not demonstrate nematicidal activity against C. elegans and antimicrobial activity at any concentration of 10–100 and 2.34–300 μg/mL, respectively.
Table 2

NMR spectroscopic data for alkaloids 1 and 2

Position

1 a

2 b

δC, type

δH, mult (J/Hz)

δC, type

δH, mult (J/Hz)

1

136.3, C

135.2, C

3

150.4, C

144.1, C

4

117.5, CH

8.38, s

118.1, CH

8.25, s

4a

135.5, C

134.8, C

4b

121.5, C

120.6, C

5

122.7, CH

8.20, d (J = 7.93)

121.9, CH

8.17, d (J = 7.53)

6

121.9, CH

7.29, dd (J = 8.09, 7.17)

120.8, CH

7.35, td (J = 7.74, 1.08)

7

130.2, CH

7.57, dd (J = 8.09, 7.16)

129.4, CH

7.62, td (J = 7.96, 1.08)

8

113.6, CH

7.67, d (J = 8.10)

112.0, CH

7.59, d (J = 7.59)

8a

143.8, C

141.5, C

9a

134.1, C

132.4, C

10

203.7, C

203.3, C

11

26.0, CH3

2.82, s

25.7, CH3

2.89, s

12

66.6, CH2

4.91, s

67.6, CH2

5.47, s

1′

170.9, O=C–O

2′

21.1, CH3

2.21, s

N-H

nd

10.27, br s

nd not detected

a1H (MeOH-d4): 500 MHz; 13C 125 MHz

b1H (CDCl3): 700 MHz; 13C 175 MHz

Notes

Acknowledgements

The National Park, Wildlife and Plant Conservation Department in Thailand is gratefully acknowledged for permission to conduct a study in the protected areas. We also thank Wera Collisi and Christel Kakoschke for conducting the cytotoxicity assay and NMR spectroscopic measurements, respectively.

Funding information

This work was supported by the TRF through the Royal Golden Jubilee Ph.D. program (grant number PHD/0163/2556 to W.K.); the Alexander von Humboldt Foundation (AvH Georg-Forster Postdoctoral Research Fellowship to A.P.G.M.); the German Federal Ministry of Education and Research (BMBF); the National Center for Genetic Engineering and Biotechnology (BIOTEC); the National Science and Technology Development Agency (NSTDA); and the Prince of Songkla University Graduate School. This project has also received funding from the European Union’s Horizon 2020 research and innovation programme (RISE) under the Marie Skłodowska-Curie grant agreement no. 645701, project acronym “GoMyTri”; lead beneficiaries J.J.L. and M.S.

Supplementary material

11557_2018_1431_MOESM1_ESM.pdf (341 kb)
ESM 1 (PDF 341 kb)

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© The Author(s) 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Authors and Affiliations

  • Wilawan Kuephadungphan
    • 1
    • 2
  • Allan Patrick G. Macabeo
    • 1
    • 3
  • Janet Jennifer Luangsa-ard
    • 4
  • Kanoksri Tasanathai
    • 4
  • Donnaya Thanakitpipattana
    • 4
  • Souwalak Phongpaichit
    • 2
  • Kamila Yuyama
    • 1
  • Marc Stadler
    • 1
    Email author
  1. 1.Department Microbial Drugs, Helmholtz Centre for Infection Research, and partner site Hannover/Braunschweig of the German Centre for Infection Research (DZIF)BraunschweigGermany
  2. 2.Department of Microbiology, Faculty of SciencePrince of Songkla UniversitySongkhlaThailand
  3. 3.Laboratory for Organic Reactivity, Discovery and Synthesis (LORDS), Research Center for the Natural and Applied SciencesUniversity of Santo TomasManilaPhilippines
  4. 4.Microbe Interaction and Ecology Laboratory, National Centre for Genetic Engineering and Biotechnology (BIOTEC), NSTDAPathum ThaniThailand

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