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Oxygenated lanostane-type triterpenes profiling in laccate Ganoderma chemotaxonomy

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Abstract

Oxygenated lanostane-type triterpenes (OLTT), including ganoderic acids and lucidenic acids produced by fungi of the genus Ganoderma (Polyporales), are abundantly documented for their potential pharmacological value. In order to test the correlation between species identity and OLTT composition, methanolic extracts of seven laccate Ganoderma species were analyzed by liquid chromatography coupled to mass spectrometry. OLTT profiles of each species were compared to a phylogenetic reconstruction of Ganoderma based on ITS rDNA sequences. The results suggest a high specificity in OLTT composition in one of the phylogenetic lineages of Ganoderma that encompasses tropical species, when no OLTT compound was detected in other lineages (including the European G. lucidum and the Asian G. sinense). Within the OLTT-positive lineage, G. sichuanense, G. martinicense, and G. tuberculosum (Asian-tropicum clade) were characterized by a specific composition in ganoderic acids and G. curtisii by a variety of lucidenic acids. An unidentified OLTT was found in G. resinaceum, also equivocally positioned in phylogenetic analyses. These results confirm OLTT as a suitable taxonomic marker in a lineage of pharmacologically and economically valuable species. Correlations with phylogeny, and development of OLTT composition as a fingerprint tool for quality control, could be an issue to address next, based on a more complete species sampling.

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Abbreviations

GA:

ganoderic acid

HPLC:

high performance liquid chromatography

HRMS:

high resolution mass spectrometry

LC–ESI–MS:

liquid chromatography–electrospray–ionization–mass spectrometry

LC–ESI–MS/MS:

liquid chromatography–electrospray ionization–mass tandem spectrometry

PCR:

polymerase chain reaction

RDB:

ring double bond

TIC:

total ion current

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Acknowledgments

We thank Jean-Paul Bonte, Jean-François Goossens, Mostafa Kouach and Brigitte Callens (CUMA), Ines Devred and Christophe Lécuru (Laboratoire des Sciences Végétales et Fongiques), Jean-François Goossens, Catherine Foulon, Nadège Schifano and Valérie Verones (Laboratoire de Chimie Analytique) for their technical assistance, Jean-Michel Bellanger (CEFE-CNRS, Montpellier) for his attempts to get DNA sequences from vouchers and Béatrice Boury (DSI, Université Lille 2) for computer assistance. We also thank David Navarro and “Le Centre Internationational de Ressources Microbiennes (CIRM)”, INRA, Aix-Marseille Université, UMR1163 BBF, ESIL Polytech. Jean-Pierre Fiard (Fort-de-France, Martinique) and Félix Lurel (Petit-Bourg, Guadeloupe) are warmly acknowledged for logistics and accommodation during field work in the French West Indies, as is Christian Lechat (Virollet, France) for field assistance. Material from Guadeloupe and Martinique was collected in the research program “Lesser Antilles Fungi; diversity, ecology and conservation”—(R. Courtecuisse) and financially supported by DIREN Guadeloupe (Regional Environment Administration—Luc Legendre), DIREN Martinique (Vincent Arenales del Campo), ONF Martinique (National Forestry Office, regional direction—Philippe Richard and Jean-Baptiste Schneider). The Parc National de Guadeloupe administration is thanked for yearly collecting authorizations in the protected areas of Guadeloupe. Cony Decock gratefully acknowledges the financial support received from the FNRS / FRFC (convention FRFC 2.4544.10) and from the Belgian State—Belgian Federal Science Policy through the BCCM™ research program. Finally, we are grateful to Pr. Terry Henkel and Dr. Michael Howsam for English revision and helpful comments.

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Authors and Affiliations

  1. EA 4483, Laboratoire des sciences végétales et fongiques, UFR Pharmacie, Université de Lille, B.P. 83, F–59006, Lille Cedex, France

    Stéphane Welti, Pierre–Arthur Moreau & Régis Courtecuisse

  2. UDSL, EA GRIIOT, UFR Pharmacie, Université de Lille, B.P. 83, F–59006, Lille Cedex, France

    Stéphane Welti, Pierre–Arthur Moreau, Cécile Danel, Nathalie Duhal & Régis Courtecuisse

  3. Centre Universitaire de Mesures et d’Analyses (C.U.M.A.), Service Commun de Physico–Chimie, UFR Pharmacie, Université de Lille, F–59006, Lille Cedex, France

    Nathalie Duhal

  4. Laboratoire de Chimie Analytique. UFR Pharmacie, Université de Lille, B.P.83, F–59006, Lille Cedex, France

    Cécile Danel

  5. Mycothèque de l’Université Catholique de Louvain (MUCL/BCCM), Earth and Life Institute—Microbiology (ELIM), Université catholique de Louvain, Croix du Sud 2 bte L7.05.06, B–1348, Louvain-la-Neuve, Belgium

    Cony Decock

  6. INRA, UMR1163 BBF, ESIL Polytech Marseille, 163 avenue de Luminy, CP 925, 13288, Marseille, Cedex 09, France

    Anne Favel

  7. UMR1163 BBF, ESIL Polytech Marseille, Aix–Marseille Université, 163 avenue de Luminy, CP 925, 13288, Marseille, Cedex 09, France

    Anne Favel

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  1. Stéphane Welti
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  2. Pierre–Arthur Moreau
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  3. Cony Decock
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  4. Cécile Danel
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  5. Nathalie Duhal
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  6. Anne Favel
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  7. Régis Courtecuisse
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Correspondence to Stéphane Welti.

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Welti, S., Moreau, P., Decock, C. et al. Oxygenated lanostane-type triterpenes profiling in laccate Ganoderma chemotaxonomy. Mycol Progress 14, 45 (2015). https://doi.org/10.1007/s11557-015-1066-7

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  • DOI: https://doi.org/10.1007/s11557-015-1066-7

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