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A simple in vitro screening method to determine the effects of drugs against growth of Saprolegnia parasitica

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Abstract

To screen the effect of possible antifungal chemicals against growth of fish pathogenic Saprolegnia spp., a simple and rapid in vitro screening method has been developed. Heat sterilized hemp seeds (Cannabis sativa) colonized by Saprolegnia parasitica are exposed to different concentrations of the test drugs diluted in water. One Saprolegnia colonized hemp seed is transferred into each well of a 48-well flat bottom tissue culture plate, after which 1 mL of each concentration of the test drugs is added per well. Subsequent to exposure and incubation, the plate is inspected and any Saprolegnia growth on the seeds is graded.

The method described in the present paper proved to be simple, effective and reproducible in screening of fungistatic and fungicidal drugs against Saprolegnia growth.

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Authors and Affiliations

  1. National Veterinary Institute, P.O. Box 8156 Dep., 0033, Oslo, Norway

    Svein Stueland, Berit Tafjord Heier & Ida Skaar

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  1. Svein Stueland
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  2. Berit Tafjord Heier
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  3. Ida Skaar
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Correspondence to Ida Skaar.

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Stueland, S., Tafjord Heier, B. & Skaar, I. A simple in vitro screening method to determine the effects of drugs against growth of Saprolegnia parasitica . Mycol Progress 4, 273–279 (2005). https://doi.org/10.1007/s11557-006-0131-7

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  • DOI: https://doi.org/10.1007/s11557-006-0131-7

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