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Estimation of phylogenetic relationships within the Ascomycota on the basis of 18S rDNA sequences and chemotaxonomy

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Abstract

Small subunit rRNA gene sequences (18S rDNA), cell wall carbohydrate composition and ubiquinone components were analysed within a larger number of ascomycetous yeasts and dimorphic fungi to validate their congruence in predicting phylogenetic relationships. The glucose-mannose pattern distinguishes the Hemiascomycetes from the Euascomycetes and the Protomycetes which are characterised with the glucose-mannose-galactose-rhamnose-(fucose) profile. The glucose-mannose-galactose pattern was found in the cell walls of all the three classes. Different coenzyme Q component (CoQ5 to CoQ10) were found within the representatives of the Hemiascomycetes. Whereas CoQ9, CoQ10 and CoQ10H2 predominate within the Euascomycetes, CoQ9 and CoQ10 characterise the Protomycetes. Chemotaxonomic studies coupled with additional molecular and co-evolution studies support the idea that the Hemiascomycetes occupy a basal position in the phylogeny of Ascomycota. These results are not in line with the phylogenetic studies based on the sequences of 18S rRNA encoding gene. The maximum parsimony analysis indicated that Hemiascomycetes and Protomycetes might represent sister groups, opposing to the earlier reported results, where the Archiascomycetes (Protomycetes) or the Hemiascomycetes had been considered to be the most primitive ascomycetous fungi. Instead of the class Archiascomycetes, the term Protomycetes was introduced reflecting much better the properties of the whole class.

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Authors and Affiliations

  1. Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, A-1190, Vienna, Austria

    Ksenija Lopandic, Orsolya Molnár & Hansjörg Prillinger

  2. Japan Collection of Microorganisms, RIKEN, Wako, Saitama, 351-0198, Japan

    Motofumi Suzuki

  3. Institut für Medizinische Biologie, Arbeitsgruppe Genetik, Universität Wien, Währingerstrasse 10, A-1090, Wien

    Wilhelm Pinsker

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  1. Ksenija Lopandic
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  2. Orsolya Molnár
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  5. Hansjörg Prillinger
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Correspondence to Hansjörg Prillinger.

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Lopandic, K., Molnár, O., Suzuki, M. et al. Estimation of phylogenetic relationships within the Ascomycota on the basis of 18S rDNA sequences and chemotaxonomy. Mycol Progress 4, 205–214 (2005). https://doi.org/10.1007/s11557-006-0124-6

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