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Complete Genome Analysis of a PVYN-Wi Recombinant Isolate from Solanum tuberosum in China

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Abstract

The complete sequence of CF_YL21, a Potato virus Y (PVY) isolate from Solanum tuberosum in China, was determined to be 9718 nucleotides in length, excluding the 3′-terminal poly(A) tail. The viral genome had a single open reading frame of 9186 nucleotides encoding a polyprotein of 3061 amino acids. The polyprotein was predicted to be cleaved into ten functional proteins by three viral proteases. Sequence analyses indicated that CF_YL21 shared 97% nucleotide identity with Wilga5 (PVYN-Wi), and two putative recombination signals were detected in the P1 and HC-Pro/P3 regions. Phylogenetic analyses, Bayesian Tip-association Significance (BaTS) test, and multiplex RT-PCR assay confirmed that the isolate had the similar molecular and genomic structure with PVYN-Wi, a PVY strain formed by recombination between PVYN and PVYO. To our knowledge, this is the first report of the complete sequence structure of PVYN-Wi strain from potato in China.

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Acknowledgements

This work was supported by the China Agriculture Research System (Grant No. CARS-10-P11) and the Fujian Natural Science Funds for Distinguished Young Scholar (Grant No. 2014J06008), People’s Republic of China. We thank Mr. Wenchao Lin for assistance in manuscript preparation.

Authors’ Contributions

JZ conceived and designed the experiments and wrote the manuscript; FC generated data and wrote the manuscript; WCZ and SZ carried out the experiments; and FLG and JGS analyzed the data. All authors reviewed the manuscript and approved the final manuscript.

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    Authors and Affiliations

    1. Fujian Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

      Fei Chang, Fangluan Gao, Wenchao Zou & Shuang Zhao

    2. Inspection & Quarantine Technology Center, Fujian Key Laboratory for Technology Research of Inspection and Quarantine, Fujian Exit-Entry Inspection and Quarantine Bureau, Fuzhou, 350001, China

      Jianguo Shen

    3. Key Laboratory for Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, Fujian, People’s Republic of China

      Jiasui Zhan

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    1. Fei Chang
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    2. Fangluan Gao
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    Correspondence to Jiasui Zhan.

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    Fei Chang and Fangluan Gao contributed equally to this work.

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    Disease symptoms induced by CF_YL21 on potato (PDF 250 kb)

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    Chang, F., Gao, F., Shen, J. et al. Complete Genome Analysis of a PVYN-Wi Recombinant Isolate from Solanum tuberosum in China. Potato Res. 58, 377–389 (2015). https://doi.org/10.1007/s11540-015-9307-3

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