Abstract
Genetic analysis established that Aitaiyin3, a dwarf rice variety derived from a semidwarf cultivar Taiyin1, carries two recessive semidwarf genes. By using simple sequence repeat (SSR) markers, we mapped the two semidwarf genes, sd-1 and sd-t2 on chromosomes 1 and 4, respectively. Sd-t2 was thus named because the semidrawf gene sd-t has already been identified from Aitaiyin 2 whose origin could be traced back to Taiyin1. The result of the molecular mapping of sd-1 gene revealed it is linked to four SSR markers found on chromosome 1. These markers are: RM297, RM302, RM212, and OSR3 spaced at 4.7 cM, 0 cM, 0.8cM and 0 cM, respectively. Sd-t2 was found to be located on chromosome 4 using five SSR markers: two markers, SSR332 and RM1305 located proximal to sd-t2 are spaced 11.6 cM, 3.8 cM, respectively, while the three distally located primers, RM5633, RM307, and RM401 are separated by distances of 0.4 cM, 0.0 cM, and 0.4 cM, respectively.
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References
Ashikari M., Wu J., Yano M., Sasaki T., Yoshimura A., Rice gibberellin-insensitive dwarf mutant gene Dwarf 1 encodes the subunit of GTP-binding protein. Proc. Natl. Acad. Sci. U.S.A., 1999, 96(18): 10284–10289
Gu M.-H. and Zhu L.-H., Genetic analysis of the allelism of several dwarf genes in Indica rice (Oryza sativa L.), Hereditas, 1979, 1(6): 10–13 [顾铭洪, 朱立宏, 几个矮秆籼稻矮秆基因等位关系的初步分析, 遗传, 1979, 1(6): 10–13]
Gu M.-H., Pan X.-B., Li X., Dong G.-C., Isolation and identification of a new semidwarf gene inIndica rice (Oryza sativa L.). Scientica Agricultura Sinica, 1988, 21(1): 33–40 [顾铭洪, 潘学彪, 李欣, 董桂春, 一种籼稻矮源的分离和遗传鉴定, 中国农业科学, 1988, 21(1): 33–40]
Gu M.-H., The dwarf resources and their utilization in rice breeding, J. of Jiangsu Agriculture College, 1980, 1(1): 40–44 [顾铭洪, 矮源及其在水稻育种上的应用, 江苏农学院学报, 1980, 1(1): 40–44].
Hedden P. and Phillips A.-L., Gibberellin metabolism: new insights revealed by the genes, Trends Plant Sci., 2000, 5(12): 523–530
Hedden P., Phillips A.-L., Rojas M.-C., Carrera E., Tudzynski B., Gibberellin biosynthesis in plants and fungi: a case of convergent Evolution? Plant Growth Regul, 2002, 20(4): 319–331
Ikeda A., Ueguchi-Tanaka M., Sonoda Y., Kitano H., Koshioka M., Futsuhara Y., Matsuoka M., Yamaguchi J., Slender rice, a constitutive gibberellin response mutant, is caused by a null mutation of the SLR1 gene, an ortholog of the Height-regulating gene GAI/RGA/RHT/D8. Plant Cell, 2001, 13(5): 999–1010
Itoh H., Ueguchi-Tanaka M., Sentoku N., Kitano H., Matsuoka M., Kobayashi M., Cloning and functional analysis of two gibberellin 3 beta-hydroxylase genes that are differently expressed during the growth of rice, Proc. Natl. Acad. Sci. U.S.A., 2001, 98(15): 8909–8914
Jiang G.-H., Liang G.-H., Zhai W.-X., Gu M.-H., Lu R.-L., Xu J.-C., Zhu L.H., Genetic mapping of a new semidwarf gene, sd-t(t), in indica rice and estimating of the physical distance of the mapping region. Sci. China (series C), 2002, 32(3): 193–199 [江广怀, 梁国华, 翟文学, 顾铭洪, 鲁润龙, 朱立宏, 籼稻中矮秆基因 sd-t 的遗传定位及定位区间物理距离的估计, 中国科学 (C 辑), 2002,32(3): 193–199]
Kinoshita T., Report of committee on gene symbolization, nomenclature and linkage groups, Rice Genetics Newsl., 1995, 12: 9–97.
Lander E.-S., Green P., Abrahamson J., Barlow A., Daly M. J., Lincoln S.-E., Newburg L., MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics, 1987, 1(2): 174–181
Liang C.-Z., Gu M.-H., Pan X.-B., Liang G.-H., Zhu L. H., RFLP tagging of a new semidwarf gene in rice, Theor. Appl. Genet., 1994, 88: 898–900.
Liang G.-H., Pan X.-B., Gu M.-H., Ji C.-Q., Isolation and identification of a semidwarf gene from an indica rice variety Aitaiyin 2. Chinese J. Rice Science, 1995, 9(5): 189–192 [梁国华, 潘学彪, 顾铭洪, 嵇朝球, 矮泰引一种半矮秆基因的分离和鉴定, 中国水稻科学, 1995, 9(5): 189–192]
McCouch S.-R., Kotcket G., Yu Z.-H., Molecular mapping of rice chromosome, Theor. Appl. Genet., 1988, 76: 815–829
McCouch S.-R., Teytelman L., Xu Y., Lobos K.-B., Clare K., Walton M., Fu B., Maghirang R., Li Z., Xing Y., Zhang Q., Kono I., Yano M., Fjellstrom R., DeClerck G., Schneider D., Cartinhour S., Ware D., Stein L., Development and mapping of 2240 new SSR markers for rice, DNA Res., 2002, 9(6): 199–207
Monna L., Kitazawa N., Yoshino R., Suzuki J., Masuda H., Machara Y., Tanji M., Sato M., Nasu S., Minobe Y., Positional cloning of rice semidwarfing gene, sd-1: rice “green revolution gene” encodes a mutant enzyme involved in gibberellin synthesis, DNA Res., 2002, 9: 11–17
Mori M., Nomura T., Ooka H., Ishizaka M., Yokota T., Sugimoto K., Okabe K., Kajiwara H., Satoh K., Yamamoto K., Hirochika H., Kikuchi S., Isolation and characterization of a rice dwarf mutant with a defect in brassinosteroid biosynthesis, Plant Physiol., 2002, 130(3): 1152–1161
Murray M.-G. and Thompson W.-F., Rapid isolation of high molecular weight plant DNA, Nucl. Acid Res., 1980, 8: 4321–4325
Peng J., Richards D.-E., Hartley N.-M., Murphy G.-P., Devos K.-M., Flintham J.-E., Beales J., Fish L.-J., Worland A.-J., Pelica F., Sudhakar D., Christou P., Snape J.-W., Gale M.-D., Harberd N.-P., “Green revolution genes” encode mutant gibberellin response modulators, Nature, 1999, 400(6741): 256–261
Sasaki A., Ashikari M., Ueguchi-Tanaka M., Itoh H., Nishimura A., Swapn D., Ishiyama K., Saito T., Kobayashi M., Khush G.-S., Kitano H., Matsuoka M., A mutant gibberellin-synthesis gene in rice, Nature, 2002, 416: 701–702
Sato Y., Sentoku N., Miura Y., Hirochika H., Kitano H., Matsuoka M., Loss-of-function mutations in the rice homeobox gene OSH15 affect the architecture of internodes resulting in dwarf plants. EMBO J., 1999, 18(4): 992–1002.
Schaller H., The role of sterols in plant growth and development, Prog. Lipid Res., 2003, 42(3): 163–175
Schomburg F.-M., Bizzell C.-M., Lee D.-J., Zeevaart J.-A., Amasino R.-M., Overexpression of a novel class of gibberellin 2-oxidases decreases gibberellin levels and creates dwarf plants, Plant Cell, 2003, 15(1): 151–163
Temnykh S., DeClerck G., Lukashova A., Lipovich L., Cartinhour S., McCouch S.-R., Computational and experimental analysis of microsatellites in rice (Oryza sativa L.): Frequency, length variation, transposon associations, and genetic marker potential, Genome Res., 2001, 11: 1441–1452
Xiong Z.-M. Min S.-K., Cheng S.-H., Genetic analysis and utilization of the dwarf resources in China, Rice Rev. Abstr., 1988, 7(4): 1–5 [熊振民, 闵绍楷, 程式华, 我国水稻矮源的研究与利用, 水稻文摘, 1988, 7(4): 1–5]
Xu Y.-L., Li L., Wu K., Peeters A.-J., Gage D.-A., Zeevaart J.-A., The GA5 locus of Arabidopsis thaliana encodes a multifunctional gibberellin 20-oxidase: molecular cloning and functional expression, Proc. Natl. Acad. Sci. U.S.A., 1995, 92(14): 6640–6644
Yamamuro C., Ihara Y., Wu X., Noguchi T., Fujioka S., Takatsuto S., Ashikari M., Kitano H., Matsuoka M., Loss of function of a rice brassinosteroid insensitive1 homolog prevents internodes elongation and bending of the lamina joint. Plant Cell, 2000, 12(9): 1591–1605
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Translated from Acta Genetica Sinica, 2005, 32 (2) [译自: 遗传学报, 2005,32(2)]
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Zhao, X., Liang, G., Zhou, J. et al. Molecular mapping of two semidwarf genes in an indica rice variety Aitaiyin3 (Oryza sativa L.). Front. Biol. China 1, 93–98 (2006). https://doi.org/10.1007/s11515-006-0012-0
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DOI: https://doi.org/10.1007/s11515-006-0012-0