Skip to main content
SpringerLink
Log in

Storage-Induced Changes in Plasticizer Emigration and Structural Properties of Soybean Isolate Protein-Based Composite Films

  • RESEARCH
  • Published:
Food Biophysics Aims and scope Submit manuscript

Abstract

Previous study found soybean isolate protein (SPI)-corn starch (CS) composite films (SPI:CS (4:1) composite film, SPI:CS (1:1) composite film) exhibited good mechanical property and better stability during the short-term storage pre-experiment. In order to accordingly understanding the causes, plasticizer emigration and structural properties of SPI-based composite films stored at different temperatures (5 ℃, 20 ℃, 35 ℃) were systematically investigated over a period of time. High storage temperature (> 20 ℃) accelerated plasticizer emigration, which reflected in increased emigration rate of glycerol molecules and decreased moisture content. Furthermore, the decreased free SH partly formed new disulfide bonds and non-covalent inter-molecular interactions (hydrogen bonds) significantly weakened the protein structure, which influenced mechanical properties changes of SPI-based composite films during storage. The polymerization and aggregation appeared in network structure of the films was bond with disulfide bonds, and plasticizer emigration promoted the reorganization into others, with prevalence of extended β-sheet conformation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. R. Battisti, N. Fronza, Á.V. Júnior, S.M. da Silveira, M.S.P. Damas, M.G.N. Quadri, Gelatin-coated paper with antimicrobial and antioxidant effect for beef packaging. Food Packag. Shelf Life 11, 115–124 (2017)

    Article  Google Scholar 

  2. E. Basiak, A. Lenart, F. Debeaufort, Effects of carbohydrate/protein ratio on the microstructure and the barrier and sorption properties of wheat starch-whey protein blend edible films. J. Sci. Food Agr. 97, 858–867 (2017)

    Article  CAS  Google Scholar 

  3. W. Huo, D. Wei, W. Zhu, Z. Li, Y. Jiang, High-elongation zein films for flexible packaging by synergistic plasticization: preparation, structure and properties. J. Cereal Sci. 79, 354–361 (2018)

    Article  CAS  Google Scholar 

  4. Y. Han, M. Yu, L. Wang, Preparation and characterization of antioxidant soy protein isolate films incorporating licorice residue extract. Food Hydrocoll. 75, 13–21 (2018)

    Article  CAS  Google Scholar 

  5. Z.A. Maryam-Adilaha, Z.A. Nur-Hanani, Storage stability of soy protein isolate films incorporated with mango kernel extract at different temperature. Food Hydrocoll. 87, 541–549 (2019)

    Article  Google Scholar 

  6. P.C. Srinivasa, M.N. Ramesh, R.N. Tharanathan, Effect of plasticizers and fatty acids on mechanical and permeability characteristics of chitosan films. Food Hydrocoll. 21, 1113–1122 (2007)

    Article  CAS  Google Scholar 

  7. Y. Zhong, S. Liu, C. Huang, X. Li, L. Chen, L. Li, J. Zhu, Effect of amylose/amylopectin ratio of esterified starch-based films on inhibition of plasticizer migration during microwave heating. Food Control 82, 283–290 (2017)

    Article  CAS  Google Scholar 

  8. L. Wang, J. Xu, M. Zhang, H. Zheng, L. Li, Preservation of soy protein-based meat analogues by using PLA/PBAT antimicrobial packaging film. Food Chem. 380, 132022 (2022)

    Article  CAS  PubMed  Google Scholar 

  9. E.M. Ciannamea, P.M. Stefani, R.A. Ruseckaite, Storage-induced changes in functional properties of glycerol plasticized-soybean protein concentrate films produced by casting. Food Hydrocolloid. 45, 247–255 (2015)

    Article  CAS  Google Scholar 

  10. Q. Wei, Y.Y. Wang, X. Yan, Y.Y. Zhang, X.F. Guo, Effect of preparing conditions on the mechanical properties of soy protein isolate/starch composite film. J. Henan Univ. Technol. (Nat. Sci. Ed.) 40, 20–24 (2019)

    Google Scholar 

  11. T. Zhu, L. Ma, Q. Li, P. Fan, Q. Wei, X. Guo, F. Chen, Effect of storage temperature on physical properties and application in packaging of the soybean isolate protein-corn starch composite films. Starch-Stärke. 11, 2200139 (2022)

    Google Scholar 

  12. X. Guo, H. Cui, The stability of zein film during storage in natural environment. Grain Process. 40, 35–36 (2015)

    Google Scholar 

  13. P. Guo, Q. Huang, Study on analytical method of free glycerin and total glycerin in biodiesel. Cereals Oils. 8, 41–42 (2003)

    Google Scholar 

  14. X. Guo, C. Ren, Y. Zhang, H. Cui, C. Shi, Stability of zein-based films and their mechanism of change during storage at different temperatures and relative humidity. J. Food Process. Pres. 44(1467), 1 (2020)

    Google Scholar 

  15. K.Y. Chan, B.P. Wasseman, Direct colorimetric assay of free thiol groups and disulfide bonds in suspensions of solubilized and particulate cereal proteins. Cereal Chem. 70, 22–26 (1993)

    CAS  Google Scholar 

  16. X. Guo, X. Sun, Y. Zhang, R. Wang, X. Yan, Interactions between soy protein hydrolyzates and wheat proteins in noodle making dough. Food Chem. 245, 500–507 (2018)

    Article  CAS  PubMed  Google Scholar 

  17. E.M. Ciannamea, P.M. Stefani, R.A. Ruseckaite, Physical and mechanical properties of compression molded and solution casting soybean protein concentrate based films. Food Hydrocoll. 38, 193–204 (2014)

    Article  CAS  Google Scholar 

  18. D.F. Hager, Effects of extrusion upon soy concentrate solubility. J. Agri. Food Chem. 32, 293–296 (1984)

    Article  CAS  Google Scholar 

  19. U.K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685 (1970)

    Article  CAS  PubMed  Google Scholar 

  20. P. Guerrero, A. Retegi, N. Gabilondo, K. de la Caba, Mechanical and thermal properties of soy protein films processed by casting and compression. J. Food Eng. 100, 145–151 (2010)

    Article  CAS  Google Scholar 

  21. N.E. Suyatma, L. Tighzert, A. Copinet, V. Coma, Effects of hydrophilic plasticizers on mechanical, thermal, and surface properties of chitosan films. J. Agri. Food Chem. 53, 3950–3957 (2005)

    Article  CAS  Google Scholar 

  22. R. Sothornvit, J.M.Krochta, Plasticizers in edible films and coatings. in Innovations in Food Packaging, Academic Press 403–433 (2005)

  23. A. Ket-On, N. Pongmongkol, A. Somwangthanaroj, T. Janjarasskul, K. Tananuwong, Properties and storage stability of whey protein edible film with spice powders. J. Food Sci. Tech. 53, 2933–2942 (2016)

    Article  CAS  Google Scholar 

  24. K. Wang, X. Li, H. Peng, H. Peng, Y. Dong, Y. Li, X. Liu, J. Li, Tough and strong soy protein film by integrating CNFs and MXene with photothermal conversion and UV-blocking performance. Cellulose 29, 9235–9249 (2022)

    Article  CAS  Google Scholar 

  25. L. Di-Gioia, S. Guilbert, Corn protein-based thermoplastic resins: effect of some polar and amphiphilic plasticizers. J. Agri. Food Chem. 47, 1254–1261 (1999)

    Article  CAS  Google Scholar 

  26. C.E. Chinma, C.C. Ariahu, J.S. Alakali, Effect of temperature and relative humidity on the water vapour permeability and mechanical properties of cassava starch and soy protein concentrate based edible films. J. Food Sci. Tech. 52, 2380–2386 (2015)

    Article  CAS  Google Scholar 

  27. J.F. Su, Z. Huang, Y.H. Zhao, X.Y. Yuan, X.Y. Wang, M. Li, Moisture sorption and water vapor permeability of soy protein isolate/poly(vinyl alcohol)/glycerol blend films. Ind. Crops Prod. 31, 266–276 (2010)

    Article  CAS  Google Scholar 

  28. E.M. Ciannamea, P.M. Stefani, R.A. Ruseckaite, Storage-induced changes in functional properties of glycerol plasticized soybean protein concentrate films produced by casting. Food Hydrocoll. 45, 247–255 (2015)

    Article  CAS  Google Scholar 

  29. R. Thakur, P. Pristijono, C.J. Scarlett, M. Bowyer, S.P. Singh, Q.V. Vuong, Starch-based films: major factors affecting their properties. Int. J. Biol. Macromol. 132, 1079–1089 (2019)

    Article  CAS  PubMed  Google Scholar 

  30. L. Liao, T.X. Liu, M.M. Zhao, H.F. Zhao, C. Cui, Aggregation behavior of wheat gluten during carboxylic acid deamidation upon hydrothermal treatment. J. Cereal Sci. 54, 129–136 (2011)

    Article  CAS  Google Scholar 

  31. S. Jiang, Y. Wei, J. Li, J. Li, X. Li, K. Wang, K. Li, S.Q. Shi, J. Li, Z. Fang, Development of a multifunctional nanocomposite film with record-high ultralow temperature toughness and unprecedented fatigue-resistance. Chem. Eng. J. 432, 134408 (2022)

    Article  CAS  Google Scholar 

  32. K. Prak, K. Nakatani, T. Katsube-Tanaka, M. Adachi, N. Maruyama, S. Utsumi, Structure-function relationships of soybean proglycinins at subunit levels. J. Agri. Food Chem. 53, 3650–3657 (2005)

    Article  CAS  Google Scholar 

  33. J.S. Chen, J. Wang, P.X. Song, X. Ma, Determination of glycinin in soybean and soybean products using a sandwich enzyme-linked immunosorbent assay. Food Chem. 162, 27–33 (2014)

    Article  CAS  PubMed  Google Scholar 

  34. A. Leerahawong, M. Tanaka, E. Okazaki, K. Osako, Stability of the physical properties of plasticized edible films from squid (Todarodes pacificus) mantle muscle during storage. J. Food Sci. 77, E159–E165 (2012)

    Article  CAS  PubMed  Google Scholar 

  35. E.M. Ciannamea, J.P. Espinosa, P.M. Stefani, R.A. Ruseckaite, Long-term stability of compression-molded soybean protein concentrate films stored under specific conditions. Food Chem. 43, 448–452 (2018)

    Article  Google Scholar 

  36. G.N. Piccirilli, M. Soazo, L.M. Pérez, N.J. Delorenzi, R.A. Verdini, Effect of storage conditions on the physicochemical characteristics of edible films based on whey protein concentrate and liquid smoke. Food Hydrocolls. 87, 221–228 (2019)

    Article  CAS  Google Scholar 

  37. X.Y. Chen, Y. Ru, F.L. Chen, X.C. Wang, X.Y. Zhao, Q. Ao, FTIR spectroscopic characterization of soy proteins obtained through AOT reverse micelles. Food Hydrocoll. 31, 435–437 (2013)

    Article  CAS  Google Scholar 

  38. M. Subirade, I. Kelly, J. Guéguen, M. Pézolet, Molecular basis of film formation from a soybean protein: comparison between the conformation of glycinin in aqueous solution and in films. Int. J. Biol. Macromol. 23, 241–249 (1998)

    Article  CAS  PubMed  Google Scholar 

Download references

Funding

This study was funded by the National Natural Science Foundation of China (No. U21A20270), the Key Scientific Research Projects of Colleges and Universities of Henan (23A550012) and the Science Foundation of Henan University of Technology (2020BS013).

Author information

Authors and Affiliations

  1. College of Food Science and Engineering, Henan University of Technology, Zhengzhou, 450001, China

    Tingwei Zhu, Jinyu Yang, Wanting Qin, Peipei Fan, Qian Wei, Xingfeng Guo & Fusheng Chen

Authors
  1. Tingwei Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jinyu Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wanting Qin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Peipei Fan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Qian Wei
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xingfeng Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Fusheng Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

TW Zhu: Conceived and designed the experiment, analyzed the data and wrote the manuscript. JY Yang and WT Qin: Performed the format of the manuscript. Q Wei: Contributed the data of the manuscript. PP Fan: Contributed the manuscript language. XF Guo: Supervised the study and helped to initiate the project. FS Chen: Supervised the study and helped to initiate the project.

Corresponding authors

Correspondence to Xingfeng Guo or Fusheng Chen.

Ethics declarations

Conflict of Interest

All authors have no competing interests of this manuscript.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhu, T., Yang, J., Qin, W. et al. Storage-Induced Changes in Plasticizer Emigration and Structural Properties of Soybean Isolate Protein-Based Composite Films. Food Biophysics 18, 533–544 (2023). https://doi.org/10.1007/s11483-023-09796-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11483-023-09796-5

Keywords

Search

Navigation