Abstract
In order to understand the crystallization behavior of complex systems such as fats, fist we need to study each component separately, and then their binary and tertiary mixtures. Here, we used X-Ray diffraction and differential scanning calorimetry to analyze the phase behavior of 2-oleoyl-1-palmitoyl-3-stearoyl-rac-glycerol (POS) and 2-linoleoyl-1-palmitoyl-3-stearoyl-rac-glycerol (PLS) binary mixtures. Both triacylglycerols were synthesized in our laboratory to obtain pure components (> 99%) and mixed in different concentration ratios to obtain 11 mixtures. The mixtures were analyzed after rapid cooling to −15 °C and after 96 h of storage at 30 °C by X-ray powder diffraction and differential scanning calorimetry. We investigated the melting temperatures and mutual interactions of sub-α, α and γ polymorphic forms of PLS with sub-α, α and pseudo-β’ polymorphic forms of POS. Under the applied conditions, we did not observe more stable polymorphic modifications of PLS. Based on the obtained data, we proposed the phase diagram of binary mixture, which can be useful for modeling a fat structuring system for food and cosmetic applications. These results provide a deeper understanding of the crystallization behavior of fats, including such a complex issue as cocoa butter crystallization.
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Acknowledgments
Authors thank V. Benešová for DSC analysis at Central laboratories UCT, Prague.
Funding
This work was supported by Technology Agency of the Czech Republic, Fund Number FW03010017. SOLID21: CZ.02.1.01/0.0/0.0/16_019/0000760 project financed the XRPD equipment.
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K. Alishevich wrote the main manuscript text. M. Berčíková together with K. Alishevich made Table 4, Fig. 2 and provide the DSC data interpretation. J. Rohlíček together with K. Alishevich made XRD analysis and interpreted the data. J. Kyselka together with J. Šmidrkal wrote 2.3 and 3.1 part of the manuscript, K. Sasínová together with T. Honzíková, P. Šimicová and K. Alishevich synthetized and purified triacylglycerols. V. Filip together with K. Alishevich designed the experimental part. All authors reviewed the manuscript.
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Alishevich, K., Berčíková, M., Kyselka, J. et al. Binary Phase Behavior of 2-oleoyl-1-palmitoyl-3-stearoyl-rac-glycerol (POS) and 2-linoleoyl-1-palmitoyl-3-stearoyl-rac-glycerol (PLS). Food Biophysics 18, 161–173 (2023). https://doi.org/10.1007/s11483-022-09761-8
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DOI: https://doi.org/10.1007/s11483-022-09761-8