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Immobilized CALB Catalyzed Transesterification of Soybean Oil and Phytosterol

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Abstract

Candida antarctica lipase B (CALB) was immobilized on Fe3O4/SiOx-g-P(GMA) polymer carrier to catalyzed the transesterification of soybean oil and phytosterol. The enzyme loading of the obtained particles was 98.7 mg/g supports and the enzyme activity was 1226.5 U/g. The average particle size was 100.5 ± 1.30 nm and the magnetization was 15.80 emu/g. The immobilized enzyme showed higher activities at a wider range of pH and temperatures. Its optimum reaction temperature was up to 50 °C; increased by 5 °C compared to the free enzyme. The obtained magnetic immobilized Fe3O4/SiOx-g-P(GMA) lipase was nanoscale. First-grade soybean oils were used as a substrate. System pH was adjusted to 7.0. The optimal reaction temperature was 50 °C and the reaction time was 3 h. The phytosterol concentration of 5% and immobilized CALB of 2% were obtained. The conversion rate of transesterification reaction between soybean oil and phytosterol was 86.2%. The use of magnets can quickly separate the immobilized enzymes from the substrates. The relative activity of the immobilized enzymes was 83.0% when reused seven times. The prepared immobilized CALB can improve efficiently enzyme activity and reutilization.

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Acknowledgements

This work was supported by a grant from the National Natural Science Foundation of China (NSFC): Study on the mechanism of nanomagnetic enzyme hydrolysis of soybean oil by multi-effect orientation and biosynthesis of functional lipids (No: 31571880).

This work was supported by a grant from the Province Natural Science Foundation of Heilong Jang: Study on the Mechanism of Continuous Orientation Esterification of Nanometer Magnetic Lipase (No: C2017019).

This work was also supported by a grant from the National Research Project in 13th Five-Year: Research on Key Technologies of green soybean oil production and large scale intelligent equipment. (No: 2016YFD0401402).

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Authors and Affiliations

  1. School of Food Science, Northeast Agricultural University, Harbin, 150030, China

    Dianyu Yu, Xu Zhang, Dezhi Zou, Tong Wang, Tianyi Liu & Lianzhou Jiang

  2. School of Computer and Information Engineering, Harbin University of Commerce, Harbin, 150028, China

    Liqi Wang

  3. UR Catalyse et Matériaux pour l’Environnement et les Procédés URCMEP (UR11ES85), Faculté des Sciences de Gabès, Université de Gabès, 6072, Gabès, Tunisia

    Walid Elfalleh

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  1. Dianyu Yu
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  2. Xu Zhang
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  3. Dezhi Zou
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  4. Tong Wang
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Correspondence to Liqi Wang, Walid Elfalleh or Lianzhou Jiang.

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Yu, D., Zhang, X., Zou, D. et al. Immobilized CALB Catalyzed Transesterification of Soybean Oil and Phytosterol. Food Biophysics 13, 208–215 (2018). https://doi.org/10.1007/s11483-018-9526-7

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  • DOI: https://doi.org/10.1007/s11483-018-9526-7

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