Abstract
This article explores the application of hyperthermia mediated by alpha human folate receptor (αHFR) targeted gold nanoparticles (GNPs) for potentiating the cytotoxicity of cyclophosphamide (CPA) in αHFR positive breast cancer cells. Folate functionalized GNPs were delivered to highly αHFR positive breast cancer cells MDA-MB-231 and to MCF-7 breast cancer cells that does not express detectable levels of αHFR followed by hyperthermia. We have shown that hyperthermia induced by folate functionalized GNPs sensitized MDA-MB-231 cells by ten-fold to CPA treatment, whereas MCF-7 cells exhibited only onefold chemosensitization. Collectively, the study suggests the feasibility of using αHFR targeted GNPs for facilitating increased cellula r uptake of CPA in cancer cells expressing elevated αHFR, allowing reduction in drug dosage.
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Acknowledgments
We would like to thank Dr. K. Satyamoorthy, the Director of Manipal Life Sciences Centre, Manipal University, India for providing us the MCF-7 and MDA-MB-231 cell lines. We would also like to thankfully acknowledge Dr. Firdos Khan, the Chairperson of School of Life Sciences, Manipal University—Dubai Campus, for his cooperation and encouragement, and the students Ms. Deepika Bhagavatula, Ms. Jia Anne Jacob, and Mr. Sanju Simon for their support.
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Banu, H., Stanley, B., Faheem, S.M. et al. Thermal Chemosensitization of Breast Cancer Cells to Cyclophosphamide Treatment Using Folate Receptor Targeted Gold Nanoparticles. Plasmonics 9, 1341–1349 (2014). https://doi.org/10.1007/s11468-014-9747-7
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DOI: https://doi.org/10.1007/s11468-014-9747-7