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Tudy on drug displacement interactions by capillary electrophoresis-frontal analysis

  • Research Article
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Frontiers of Chemistry in China

Abstract

The interaction between 18-methyl norethindrone and ketoprofen, including the displacement of ketoprofen from human serum albumin binding sites, was investigated by the capillary electrophoresis-frontal analysis method (CE-FA) at room temperature. A very large sample plug was introduced hydrostatically into the capillary (65 cm × 50 μm i.d.; effective length of 35 cm) over 80 s at a height difference of 11 cm. The working conditions for CE-FA separation are as follows: operating voltage, 10 kV; running buffer, 67 mmol·L−1 phosphate, pH 7.4. The unbound ketoprofen concentration was directly measured from the height of the frontal peak. When the concentration of 18-methyl norethindrone was increased from 0 to 200 μmol/L, the unbound ketoprofen concentration was found to increase from 22.4 to 26.4 μmol/L at 100 μmol/L total ketoprofen concentration and from 82.1 to 106.2 μmol/L at 200 μmol/L total ketoprofen concentration. From these data, it may be deduced that the administration of high concentration of 18-methyl norethindrone can displace ketoprofen from its secondary binding site.

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Authors and Affiliations

  1. Pharmaceutical analysis department, Tianjin University, Tianjin, 300072, China

    Zhou Dawei & Li Famei

Authors
  1. Zhou Dawei
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  2. Li Famei
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Correspondence to Zhou Dawei.

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Translated from Chinese Journal of Chromatography, 2005, 23(2)(in Chinese)

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Zhou, D., Famei, L. Tudy on drug displacement interactions by capillary electrophoresis-frontal analysis. Front. Chem. China 1, 207–210 (2006). https://doi.org/10.1007/s11458-006-0009-x

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  • DOI: https://doi.org/10.1007/s11458-006-0009-x

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