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Applications of HPLC-MS in compound Ilex pubescens extract study

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  • Published:
Frontiers of Chemistry in China

Abstract

In this paper, high performance liquid chromatography (HPLC) along with mass spectrometry (MS) and HPLC along with a diode array detector (DAD) was used to study the compound Ilex pubescens extract. Two ionization techniques: electro spray ionization (ESI) and atmospheric pressure chemical ionization (APCI) were used in this work. The liquid chromatograms obtained by DAD, total ion chromatograms (TIC) from positive-and negative-ion ESI-MS and the positive-and negative-ion APCI-MS were compared. The liquid chromatograms obtained by TIC from ESI-MS provided more information on chromatographic peaks than those obtained by DAD or TIC from APCI-MS. It is suggested that the fingerprints of the compound Ilex pubescens extract should be provided by the liquid chromatograms obtained by DAD together with TIC from the negative-ion ESI-MS. The molecular weights of the nine main components in an HPLC-DAD chromatogram were determined by the corresponding positive-and negative-ion ESI and the positive-and negative-ion APCI mass spectra information. In the liquid chromatogram obtained by TIC from the negative-ion ESI-MS, the molecular weights of 23 main components were determined based on the corresponding positive-and negative-ion ESI mass spectra information.

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Authors and Affiliations

  1. Guangdong Key Laboratory of Chemical Emergency Test, China National Analytical Center, Guangzhou, 510070, China

    Yang Yunyun, Hongwei Chen, Teng Jiuwei & Mu Dehai

  2. Institute of Analytical Sciences, School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou, 510275, China

    Zhong Ming & Ma Zhiling

Authors
  1. Yang Yunyun
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  2. Hongwei Chen
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  3. Zhong Ming
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  4. Ma Zhiling
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  5. Teng Jiuwei
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  6. Mu Dehai
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Correspondence to Yang Yunyun.

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Yang, Y., Hongwei, C., Zhong, M. et al. Applications of HPLC-MS in compound Ilex pubescens extract study. Front. Chem. China 1, 138–144 (2006). https://doi.org/10.1007/s11458-006-0005-1

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  • DOI: https://doi.org/10.1007/s11458-006-0005-1

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